##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII BcgI BciVI BclI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI HpaI KpnI
MamI MfeI MluI MunI NaeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 1164 TCCGGA T^CCGGA
AflII 4141 CTTAAG C^TTAAG
AflIII 927 ACGTGT A^C[GA][CT]GT
AhdI 403 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 403 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2330 GGATCC G^GATCC
BbvCI 2392 CCTCAGC CCTCAGC(-5/-2)
BfrI 4141 CTTAAG C^TTAAG
BseAI 1164 TCCGGA T^CCGGA
BspEI 1164 TCCGGA T^CCGGA
BsrFI 3905 ACCGGC [GA]^CCGG[CT]
Cfr10I 3905 ACCGGC [GA]^CCGG[CT]
DraIII 2594 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 403 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 160 GAATTC G^AATTC
EcoRV 2375 GATATC GAT^ATC
HindIII 4711 AAGCTT A^AGCTT
KspI 1987 CCGCGG CCGC^GG
MroI 1164 TCCGGA T^CCGGA
NheI 1561 GCTAGC G^CTAGC
PaeR7I 8 CTCGAG C^TCGAG
PshAI 2027 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1987 CCGCGG CCGC^GG
SspI 3279 AATATT AAT^ATT
XbaI 4190 TCTAGA T^CTAGA
XhoI 8 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 1693 GTGCAC G^TGCAC
2640 GTGCAC
ApaLI 1693 GTGCAC G^TGCAC
2640 GTGCAC
AspI 3031 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3504 GACCTTGTC
BbeI 1395 GGCGCC GGCGC^C
2669 GGCGCC
BglI 1579 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2067 GCCCTGGGGGC
BlpI 665 GCTGAGC GC^T[ACGT]AGC
1836 GCTGAGC
Bpu1102I 665 GCTGAGC GC^T[ACGT]AGC
1836 GCTGAGC
BspMI 1951 ACCTGC ACCTGC(4/8)
2268 ACCTGC
BsrDI 4548 CATTGC GCAATG(2/0)
2766 GCAATG
CelII 665 GCTGAGC GC^T[ACGT]AGC
1836 GCTGAGC
DraI 1661 TTTAAA TTT^AAA
4460 TTTAAA
EcoNI 490 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
707 CCTGTGCCAGG
KasI 1395 GGCGCC G^GCGCC
2669 GGCGCC
NarI 1395 GGCGCC GG^CGCC
2669 GGCGCC
NdeI 2774 CATATG CA^TATG
3572 CATATG
NspI 1681 GCATGT [GA]CATG^[CT]
3679 GCATGT
PflFI 3031 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3504 GACCTTGTC
PflMI 425 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4684 CCATGCTCTGG
PstI 1863 CTGCAG CTGCA^G
1953 CTGCAG
ScaI 604 AGTACT AGT^ACT
633 AGTACT
SfoI 1395 GGCGCC GGC^GCC
2669 GGCGCC
SmaI 53 CCCGGG CCC^GGG
897 CCCGGG
SpeI 2144 ACTAGT A^CTAGT
3235 ACTAGT
Tth111I 3031 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3504 GACCTTGTC
Van91I 425 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4684 CCATGCTCTGG
XmaCI 53 CCCGGG C^CCGGG
897 CCCGGG
XmaI 53 CCCGGG C^CCGGG
897 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglII
BlnI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PmaCI PmlI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII