##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AccIII AgeI Aor51HI AscI
Asp718I AviII AvrII BciVI BlnI
BseAI BspDI BspEI BssHII Bst1107I
BstZ17I ClaI CpoI Eco47III EcoRV
FseI FspI KpnI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmeI PvuI
RsrII SbfI ScaI SfiI SgrAI
SnaBI SpeI Sse8387I SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3796 GTCGAC GT^[AC][GT]AC
AclI 63 AACGTT AA^CGTT
Alw44I 2691 GTGCAC G^TGCAC
ApaI 4836 GGGCCC GGGCC^C
ApaLI 2691 GTGCAC G^TGCAC
AspI 2302 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1633 TGGCCA TGG^CCA
BbrPI 1546 CACGTG CAC^GTG
BbvCI 2544 GCTGAGG CCTCAGC(-5/-2)
BglI 2085 GCCGTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1546 CACGTG [CT]AC^GT[GA]
BsaWI 666 ACCGGA [AT]^CCGG[AT]
BsiWI 1742 CGTACG C^GTACG
BspHI 1202 TCATGA T^CATGA
BstBI 1901 TTCGAA TT^CGAA
DrdI 517 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 3180 CCAAGG C^C[AT][AT]GG
HpaI 799 GTTAAC GTT^AAC
KspI 3415 CCGCGG CCGC^GG
MluNI 1633 TGGCCA TGG^CCA
MscI 1633 TGGCCA TGG^CCA
NspV 1901 TTCGAA TT^CGAA
PflFI 2302 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1546 CACGTG CAC^GTG
PmlI 1546 CACGTG CAC^GTG
PshAI 4556 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 63 AACGTT AA^CGTT
RcaI 1202 TCATGA T^CATGA
SacII 3415 CCGCGG CCGC^GG
SalI 3796 GTCGAC G^TCGAC
SexAI 1262 ACCTGGT A^CC[AT]GGT
SfuI 1901 TTCGAA TT^CGAA
StyI 3180 CCAAGG C^C[AT][AT]GG
Tth111I 2302 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3894 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 3416 CGCG CG^CG
4919 CGCG
AflII 3026 CTTAAG C^TTAAG
3080 CTTAAG
AseI 198 ATTAAT AT^TAAT
2440 ATTAAT
AsnI 198 ATTAAT AT^TAAT
2440 ATTAAT
Asp700I 4635 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4978 GAAGCTCTTC
BamHI 288 GGATCC G^GATCC
1824 GGATCC
BbeI 1357 GGCGCC GGCGC^C
3937 GGCGCC
BcgI 1025 CGACGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2161 CGAGTCAACTGC
BclI 897 TGATCA T^GATCA
4782 TGATCA
BfrI 3026 CTTAAG C^TTAAG
3080 CTTAAG
BglII 53 AGATCT A^GATCT
1365 AGATCT
BlpI 2120 GCTCAGC GC^T[ACGT]AGC
3389 GCTGAGC
Bpu1102I 2120 GCTCAGC GC^T[ACGT]AGC
3389 GCTGAGC
BsiEI 615 CGGCCG CG[GA][CT]^CG
3412 CGGCCG
BsmBI 401 GAGACG CGTCTC(1/5)
1580 GAGACG
BsmI 2420 GAATGC GAATGC(1/-1)
1913 GCATTC
BspLU11I 4495 ACATGT A^CATGT
4511 ACATGT
BsrBI 2118 CCGCTC CCGCTC(-3/-3)
1 GAGCGG
BsrDI 1171 CATTGC GCAATG(2/0)
2052 CATTGC
BstAPI 153 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3097 GCAGAGCGTGC
BstEII 1761 GGTGACC G^GT[ACGT]ACC
1888 GGTGACC
BstPI 1761 GGTGACC G^GT[ACGT]ACC
1888 GGTGACC
BstUI 3416 CGCG CG^CG
4919 CGCG
BstXI 131 CCAAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCAAACCACTGG
CelII 2120 GCTCAGC GC^T[ACGT]AGC
3389 GCTGAGC
DraIII 2082 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2190 CACTGCGTG
EagI 615 CGGCCG C^GGCCG
3412 CGGCCG
EclXI 615 CGGCCG C^GGCCG
3412 CGGCCG
Eco52I 615 CGGCCG C^GGCCG
3412 CGGCCG
EcoNI 741 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1071 CCTGCTTCAGG
EcoO65I 1761 GGTGACC G^GT[ACGT]ACC
1888 GGTGACC
EcoRI 582 GAATTC G^AATTC
1727 GAATTC
FbaI 897 TGATCA T^GATCA
4782 TGATCA
HaeII 1357 GGCGCC [GA]GCGC^[CT]
3937 GGCGCC
KasI 1357 GGCGCC G^GCGCC
3937 GGCGCC
MvnI 3416 CGCG CG^CG
4919 CGCG
NarI 1357 GGCGCC GG^CGCC
3937 GGCGCC
PshBI 198 ATTAAT AT^TAAT
2440 ATTAAT
SfoI 1357 GGCGCC GGC^GCC
3937 GGCGCC
SspI 2508 AATATT AAT^ATT
4898 AATATT
XmnI 4635 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4978 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApoI AspEI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI Eco81I EcoO109I EcoRII EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI SspBI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI