##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI AvrII
BbrPI BciVI BclI BfrI BlnI
BsaBI BseAI BspDI BspEI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
EcoRI EcoRV EcoT22I FbaI FseI
HpaI MamI MluI MroI NdeI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SgrAI
SpeI SphI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3019 GACGTC GACGT^C
AccI 3261 GTAGAC GT^[AC][GT]AC
AhdI 3235 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 684 GTGCAC G^TGCAC
Aor51HI 2743 AGCGCT AGC^GCT
ApaLI 684 GTGCAC G^TGCAC
Asp700I 3888 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3235 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3016 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3577 TGCGCA TGC^GCA
BbeI 604 GGCGCC GGCGC^C
BcgI 3746 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiWI 2198 CGTACG C^GTACG
BsmBI 4768 GAGACG CGTCTC(1/5)
Bsp1407I 3836 TGTACA T^GTACA
BspLU11I 3270 ACATGT A^CATGT
BsrGI 3836 TGTACA T^GTACA
BssSI 354 CACGAG CACGAG(-5/-1)
BstBI 4128 TTCGAA TT^CGAA
BstEII 3242 GGTCACC G^GT[ACGT]ACC
BstPI 3242 GGTCACC G^GT[ACGT]ACC
BstXI 329 CCAGTATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 748 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1032 CGGCCG C^GGCCG
Eam1105I 3235 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1032 CGGCCG C^GGCCG
Eco47III 2743 AGCGCT AGC^GCT
Eco52I 1032 CGGCCG C^GGCCG
EcoO65I 3242 GGTCACC G^GT[ACGT]ACC
FspI 3577 TGCGCA TGC^GCA
KasI 604 GGCGCC G^GCGCC
KspI 4 CCGCGG CCGC^GG
MfeI 4581 CAATTG C^AATTG
MunI 4581 CAATTG C^AATTG
NaeI 964 GCCGGC GCC^GGC
NarI 604 GGCGCC GG^CGCC
NgoMIV 964 GCCGGC G^CCGGC
NotI 1031 GCGGCCGC GC^GGCCGC
NspI 3270 ACATGT [GA]CATG^[CT]
NspV 4128 TTCGAA TT^CGAA
PflFI 3016 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 4 CCGCGG CCGC^GG
SbfI 443 CCTGCAGG CCTGCA^GG
SexAI 1217 ACCTGGT A^CC[AT]GGT
SfiI 3821 GGCCCTCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 604 GGCGCC GGC^GCC
SfuI 4128 TTCGAA TT^CGAA
SnaBI 3417 TACGTA TAC^GTA
Sse8387I 443 CCTGCAGG CCTGCA^GG
SspBI 3836 TGTACA T^GTACA
Tth111I 3016 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2678 TCTAGA T^CTAGA
XmnI 3888 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 363 TGGCCA TGG^CCA
2074 TGGCCA
BglII 3872 AGATCT A^GATCT
3911 AGATCT
BsaAI 3288 CACGTA [CT]AC^GT[GA]
3417 TACGTA
BsaWI 970 ACCGGA [AT]^CCGG[AT]
4564 ACCGGA
BsiEI 7 CGGTCG CG[GA][CT]^CG
1032 CGGCCG
BsmI 1392 GAATGC GAATGC(1/-1)
2260 GAATGC
BspHI 2000 TCATGA T^CATGA
4738 TCATGA
BsrDI 724 CATTGC GCAATG(2/0)
3135 CATTGC
HincII 1541 GTTGAC GT[CT]^[GA]AC
2189 GTTGAC
HindII 1541 GTTGAC GT[CT]^[GA]AC
2189 GTTGAC
HindIII 2534 AAGCTT A^AGCTT
2638 AAGCTT
MluNI 363 TGGCCA TGG^CCA
2074 TGGCCA
MscI 363 TGGCCA TGG^CCA
2074 TGGCCA
NheI 398 GCTAGC G^CTAGC
3185 GCTAGC
RcaI 2000 TCATGA T^CATGA
4738 TCATGA
SapI 2520 GAAGAGC GCTCTTC(1/4)
4357 GAAGAGC
ScaI 3249 AGTACT AGT^ACT
4446 AGTACT
Tsp509I 4582 AATT ^AATT
4800 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI Asp718I AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI