##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AcyI AhdI Aor51HI
AscI AspEI AspI AviII BbeI
BcgI BglII BlpI Bpu1102I BsaHI
BsmBI BspDI BssSI BstEII BstPI
CelII ClaI DraIII Eam1105I Eco47III
EcoO65I EcoRV FseI FspI Hin1I
HincII HindII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NheI
NruI PacI PflFI PflMI PmeI
PshAI Psp1406I PvuI SalI SbfI
SfiI SfoI SgrAI SnaBI Sse8387I
SwaI Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 5252 GGTACC G^GTACC
AccIII 889 TCCGGA T^CCGGA
AflIII 3490 ACATGT A^C[GA][CT]GT
AgeI 1782 ACCGGT A^CCGGT
Alw44I 2077 GTGCAC G^TGCAC
ApaI 1637 GGGCCC GGGCC^C
ApaLI 2077 GTGCAC G^TGCAC
Asp718I 5252 GGTACC G^GTACC
BalI 5109 TGGCCA TGG^CCA
BbrPI 2659 CACGTG CAC^GTG
BbvCI 5644 CCTCAGC CCTCAGC(-5/-2)
BglI 5221 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2659 CACGTG [CT]AC^GT[GA]
BsaBI 851 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 5675 GGTCTC GGTCTC(1/5)
BseAI 889 TCCGGA T^CCGGA
BsiEI 127 CGGCCG CG[GA][CT]^CG
BsiWI 1146 CGTACG C^GTACG
BspEI 889 TCCGGA T^CCGGA
BspLU11I 3490 ACATGT A^CATGT
BsrFI 1782 ACCGGT [GA]^CCGG[CT]
BstBI 1134 TTCGAA TT^CGAA
Cfr10I 1782 ACCGGT [GA]^CCGG[CT]
CpoI 155 CGGACCG CG^G[AT]CCG
DrdI 5428 GACTGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 127 CGGCCG C^GGCCG
EclXI 127 CGGCCG C^GGCCG
Eco52I 127 CGGCCG C^GGCCG
EcoNI 2609 CCTGCACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1355 GAATTC G^AATTC
HaeII 1538 GGCGCT [GA]GCGC^[CT]
KpnI 5252 GGTACC GGTAC^C
MamI 851 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3245 CAATTG C^AATTG
MluNI 5109 TGGCCA TGG^CCA
MroI 889 TCCGGA T^CCGGA
MscI 5109 TGGCCA TGG^CCA
MunI 3245 CAATTG C^AATTG
NotI 126 GCGGCCGC GC^GGCCGC
NspV 1134 TTCGAA TT^CGAA
PaeR7I 4501 CTCGAG C^TCGAG
PinAI 1782 ACCGGT A^CCGGT
PmaCI 2659 CACGTG CAC^GTG
PmlI 2659 CACGTG CAC^GTG
RsrII 155 CGGACCG CG^G[AT]CCG
SfuI 1134 TTCGAA TT^CGAA
SpeI 5498 ACTAGT A^CTAGT
StuI 4567 AGGCCT AGG^CCT
XcmI 685 CCAGATCCTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4501 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 437 CTTAAG C^TTAAG
4473 CTTAAG
AlwNI 1268 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2056 CAGTCTCTG
AocI 4331 CCTCAGG CC^T[ACGT]AGG
4874 CCTTAGG
AseI 5358 ATTAAT AT^TAAT
5793 ATTAAT
AsnI 5358 ATTAAT AT^TAAT
5793 ATTAAT
BamHI 856 GGATCC G^GATCC
1240 GGATCC
BciVI 2773 GGATAC GTATCC(6/5)
4141 GGATAC
BfrI 437 CTTAAG C^TTAAG
4473 CTTAAG
BsmI 2962 GAATGC GAATGC(1/-1)
1663 GCATTC
BsrDI 525 CATTGC GCAATG(2/0)
3215 GCAATG
Bst1107I 3358 GTATAC GTA^TAC
4416 GTATAC
BstAPI 1267 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2957 GCACTGAATGC
BstZ17I 3358 GTATAC GTA^TAC
4416 GTATAC
Bsu36I 4331 CCTCAGG CC^T[ACGT]AGG
4874 CCTTAGG
EaeI 127 CGGCCG [CT]^GGCC[GA]
5109 TGGCCA
Eco81I 4331 CCTCAGG CC^T[ACGT]AGG
4874 CCTTAGG
MaeII 1145 ACGT A^CGT
2660 ACGT
PshBI 5358 ATTAAT AT^TAAT
5793 ATTAAT
PvuII 4277 CAGCTG CAG^CTG
5741 CAGCTG
SapI 2596 GAAGAGC GCTCTTC(1/4)
2719 GCTCTTC
SexAI 20 ACCAGGT A^CC[AT]GGT
3301 ACCTGGT
SmaI 1571 CCCGGG CCC^GGG
5256 CCCGGG
SphI 1905 GCATGC GCATG^C
3457 GCATGC
XmaCI 1571 CCCGGG C^CCGGG
5256 CCCGGG
XmaI 1571 CCCGGG C^CCGGG
5256 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII AvrII BanI BanII
BbsI BbvI BclI BcnI BfaI
BlnI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
RcaI RsaI SacI SacII Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI