################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AcyI AhdI Aor51HI AscI AspEI AspI AviII BbeI BcgI BglII BlpI Bpu1102I BsaHI BsmBI BspDI BssSI BstEII BstPI CelII ClaI DraIII Eam1105I Eco47III EcoO65I EcoRV FseI FspI Hin1I HincII HindII HpaI KasI MluI NaeI NarI NdeI NgoMIV NheI NruI PacI PflFI PflMI PmeI PshAI Psp1406I PvuI SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 5252 GGTACC G^GTACC AccIII 889 TCCGGA T^CCGGA AflIII 3490 ACATGT A^C[GA][CT]GT AgeI 1782 ACCGGT A^CCGGT Alw44I 2077 GTGCAC G^TGCAC ApaI 1637 GGGCCC GGGCC^C ApaLI 2077 GTGCAC G^TGCAC Asp718I 5252 GGTACC G^GTACC BalI 5109 TGGCCA TGG^CCA BbrPI 2659 CACGTG CAC^GTG BbvCI 5644 CCTCAGC CCTCAGC(-5/-2) BglI 5221 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2659 CACGTG [CT]AC^GT[GA] BsaBI 851 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 5675 GGTCTC GGTCTC(1/5) BseAI 889 TCCGGA T^CCGGA BsiEI 127 CGGCCG CG[GA][CT]^CG BsiWI 1146 CGTACG C^GTACG BspEI 889 TCCGGA T^CCGGA BspLU11I 3490 ACATGT A^CATGT BsrFI 1782 ACCGGT [GA]^CCGG[CT] BstBI 1134 TTCGAA TT^CGAA Cfr10I 1782 ACCGGT [GA]^CCGG[CT] CpoI 155 CGGACCG CG^G[AT]CCG DrdI 5428 GACTGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 127 CGGCCG C^GGCCG EclXI 127 CGGCCG C^GGCCG Eco52I 127 CGGCCG C^GGCCG EcoNI 2609 CCTGCACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1355 GAATTC G^AATTC HaeII 1538 GGCGCT [GA]GCGC^[CT] KpnI 5252 GGTACC GGTAC^C MamI 851 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3245 CAATTG C^AATTG MluNI 5109 TGGCCA TGG^CCA MroI 889 TCCGGA T^CCGGA MscI 5109 TGGCCA TGG^CCA MunI 3245 CAATTG C^AATTG NotI 126 GCGGCCGC GC^GGCCGC NspV 1134 TTCGAA TT^CGAA PaeR7I 4501 CTCGAG C^TCGAG PinAI 1782 ACCGGT A^CCGGT PmaCI 2659 CACGTG CAC^GTG PmlI 2659 CACGTG CAC^GTG RsrII 155 CGGACCG CG^G[AT]CCG SfuI 1134 TTCGAA TT^CGAA SpeI 5498 ACTAGT A^CTAGT StuI 4567 AGGCCT AGG^CCT XcmI 685 CCAGATCCTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 4501 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflII 437 CTTAAG C^TTAAG 4473 CTTAAG AlwNI 1268 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2056 CAGTCTCTG AocI 4331 CCTCAGG CC^T[ACGT]AGG 4874 CCTTAGG AseI 5358 ATTAAT AT^TAAT 5793 ATTAAT AsnI 5358 ATTAAT AT^TAAT 5793 ATTAAT BamHI 856 GGATCC G^GATCC 1240 GGATCC BciVI 2773 GGATAC GTATCC(6/5) 4141 GGATAC BfrI 437 CTTAAG C^TTAAG 4473 CTTAAG BsmI 2962 GAATGC GAATGC(1/-1) 1663 GCATTC BsrDI 525 CATTGC GCAATG(2/0) 3215 GCAATG Bst1107I 3358 GTATAC GTA^TAC 4416 GTATAC BstAPI 1267 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2957 GCACTGAATGC BstZ17I 3358 GTATAC GTA^TAC 4416 GTATAC Bsu36I 4331 CCTCAGG CC^T[ACGT]AGG 4874 CCTTAGG EaeI 127 CGGCCG [CT]^GGCC[GA] 5109 TGGCCA Eco81I 4331 CCTCAGG CC^T[ACGT]AGG 4874 CCTTAGG MaeII 1145 ACGT A^CGT 2660 ACGT PshBI 5358 ATTAAT AT^TAAT 5793 ATTAAT PvuII 4277 CAGCTG CAG^CTG 5741 CAGCTG SapI 2596 GAAGAGC GCTCTTC(1/4) 2719 GCTCTTC SexAI 20 ACCAGGT A^CC[AT]GGT 3301 ACCTGGT SmaI 1571 CCCGGG CCC^GGG 5256 CCCGGG SphI 1905 GCATGC GCATG^C 3457 GCATGC XmaCI 1571 CCCGGG C^CCGGG 5256 CCCGGG XmaI 1571 CCCGGG C^CCGGG 5256 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII AvrII BanI BanII BbsI BbvI BclI BcnI BfaI BlnI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RcaI RsaI SacI SacII Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI