##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AclI        AcyI        AhdI        Aor51HI     
AscI        AspEI       AspI        AviII       BbeI        
BcgI        BglII       BlpI        Bpu1102I    BsaHI       
BsmBI       BspDI       BssSI       BstEII      BstPI       
CelII       ClaI        DraIII      Eam1105I    Eco47III    
EcoO65I     EcoRV       FseI        FspI        Hin1I       
HincII      HindII      HpaI        KasI        MluI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NruI        PacI        PflFI       PflMI       PmeI        
PshAI       Psp1406I    PvuI        SalI        SbfI        
SfiI        SfoI        SgrAI       SnaBI       Sse8387I    
SwaI        Tth111I     Van91I      XbaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       5252  GGTACC               G^GTACC
AccIII        889  TCCGGA               T^CCGGA
AflIII       3490  ACATGT               A^C[GA][CT]GT
AgeI         1782  ACCGGT               A^CCGGT
Alw44I       2077  GTGCAC               G^TGCAC
ApaI         1637  GGGCCC               GGGCC^C
ApaLI        2077  GTGCAC               G^TGCAC
Asp718I      5252  GGTACC               G^GTACC
BalI         5109  TGGCCA               TGG^CCA
BbrPI        2659  CACGTG               CAC^GTG
BbvCI        5644  CCTCAGC              CCTCAGC(-5/-2)
BglI         5221  GCCCCAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        2659  CACGTG               [CT]AC^GT[GA]
BsaBI         851  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         5675  GGTCTC               GGTCTC(1/5)
BseAI         889  TCCGGA               T^CCGGA
BsiEI         127  CGGCCG               CG[GA][CT]^CG
BsiWI        1146  CGTACG               C^GTACG
BspEI         889  TCCGGA               T^CCGGA
BspLU11I     3490  ACATGT               A^CATGT
BsrFI        1782  ACCGGT               [GA]^CCGG[CT]
BstBI        1134  TTCGAA               TT^CGAA
Cfr10I       1782  ACCGGT               [GA]^CCGG[CT]
CpoI          155  CGGACCG              CG^G[AT]CCG
DrdI         5428  GACTGAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          127  CGGCCG               C^GGCCG
EclXI         127  CGGCCG               C^GGCCG
Eco52I        127  CGGCCG               C^GGCCG
EcoNI        2609  CCTGCACTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1355  GAATTC               G^AATTC
HaeII        1538  GGCGCT               [GA]GCGC^[CT]
KpnI         5252  GGTACC               GGTAC^C
MamI          851  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3245  CAATTG               C^AATTG
MluNI        5109  TGGCCA               TGG^CCA
MroI          889  TCCGGA               T^CCGGA
MscI         5109  TGGCCA               TGG^CCA
MunI         3245  CAATTG               C^AATTG
NotI          126  GCGGCCGC             GC^GGCCGC
NspV         1134  TTCGAA               TT^CGAA
PaeR7I       4501  CTCGAG               C^TCGAG
PinAI        1782  ACCGGT               A^CCGGT
PmaCI        2659  CACGTG               CAC^GTG
PmlI         2659  CACGTG               CAC^GTG
RsrII         155  CGGACCG              CG^G[AT]CCG
SfuI         1134  TTCGAA               TT^CGAA
SpeI         5498  ACTAGT               A^CTAGT
StuI         4567  AGGCCT               AGG^CCT
XcmI          685  CCAGATCCTCGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         4501  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflII         437  CTTAAG               C^TTAAG
             4473  CTTAAG              
AlwNI        1268  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2056  CAGTCTCTG           
AocI         4331  CCTCAGG              CC^T[ACGT]AGG
             4874  CCTTAGG             
AseI         5358  ATTAAT               AT^TAAT
             5793  ATTAAT              
AsnI         5358  ATTAAT               AT^TAAT
             5793  ATTAAT              
BamHI         856  GGATCC               G^GATCC
             1240  GGATCC              
BciVI        2773  GGATAC               GTATCC(6/5)
             4141  GGATAC              
BfrI          437  CTTAAG               C^TTAAG
             4473  CTTAAG              
BsmI         2962  GAATGC               GAATGC(1/-1)
             1663  GCATTC              
BsrDI         525  CATTGC               GCAATG(2/0)
             3215  GCAATG              
Bst1107I     3358  GTATAC               GTA^TAC
             4416  GTATAC              
BstAPI       1267  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2957  GCACTGAATGC         
BstZ17I      3358  GTATAC               GTA^TAC
             4416  GTATAC              
Bsu36I       4331  CCTCAGG              CC^T[ACGT]AGG
             4874  CCTTAGG             
EaeI          127  CGGCCG               [CT]^GGCC[GA]
             5109  TGGCCA              
Eco81I       4331  CCTCAGG              CC^T[ACGT]AGG
             4874  CCTTAGG             
MaeII        1145  ACGT                 A^CGT
             2660  ACGT                
PshBI        5358  ATTAAT               AT^TAAT
             5793  ATTAAT              
PvuII        4277  CAGCTG               CAG^CTG
             5741  CAGCTG              
SapI         2596  GAAGAGC              GCTCTTC(1/4)
             2719  GCTCTTC             
SexAI          20  ACCAGGT              A^CC[AT]GGT
             3301  ACCTGGT             
SmaI         1571  CCCGGG               CCC^GGG
             5256  CCCGGG              
SphI         1905  GCATGC               GCATG^C
             3457  GCATGC              
XmaCI        1571  CCCGGG               C^CCGGG
             5256  CCCGGG              
XmaI         1571  CCCGGG               C^CCGGG
             5256  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        ApoI        Asp700I     AspHI       
AvaI        AvaII       AvrII       BanI        BanII       
BbsI        BbvI        BclI        BcnI        BfaI        
BlnI        BmyI        BpmI        BpuAI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspHI       BspMI       BsrBI       BsrGI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PleI        PpuMI       PstI        
RcaI        RsaI        SacI        SacII       Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI