##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I BalI BciVI
BfrI BglI BlpI Bpu1102I BsiWI
BspDI BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraIII EcoRI
FseI HpaI KpnI MluI MluNI
MscI NcoI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3809 GACGTC GACGT^C
AccIII 3773 TCCGGA T^CCGGA
Alw44I 2899 GTGCAC G^TGCAC
AlwNI 1089 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3733 CCTAAGG CC^T[ACGT]AGG
ApaI 58 GGGCCC GGGCC^C
ApaLI 2899 GTGCAC G^TGCAC
AvrII 1756 CCTAGG C^CTAGG
BbrPI 1638 CACGTG CAC^GTG
BbvCI 2837 CCTCAGC CCTCAGC(-5/-2)
BlnI 1756 CCTAGG C^CTAGG
BsaBI 4499 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3773 TCCGGA T^CCGGA
BsiEI 33 CGGCCG CG[GA][CT]^CG
BsmI 3111 GAATGC GAATGC(1/-1)
BspEI 3773 TCCGGA T^CCGGA
BspHI 2732 TCATGA T^CATGA
BspLU11I 1653 ACATGT A^CATGT
BssHII 972 GCGCGC G^CGCGC
BstEII 3093 GGTGACC G^GT[ACGT]ACC
BstPI 3093 GGTGACC G^GT[ACGT]ACC
Bsu36I 3733 CCTAAGG CC^T[ACGT]AGG
EagI 33 CGGCCG C^GGCCG
EclXI 33 CGGCCG C^GGCCG
Eco52I 33 CGGCCG C^GGCCG
Eco81I 3733 CCTAAGG CC^T[ACGT]AGG
EcoO65I 3093 GGTGACC G^GT[ACGT]ACC
EcoT22I 4454 ATGCAT ATGCA^T
HincII 1932 GTTGAC GT[CT]^[GA]AC
HindII 1932 GTTGAC GT[CT]^[GA]AC
MamI 4499 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 703 CAATTG C^AATTG
MroI 3773 TCCGGA T^CCGGA
MunI 703 CAATTG C^AATTG
NheI 4574 GCTAGC G^CTAGC
NsiI 4454 ATGCAT ATGCA^T
PflMI 3256 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1638 CACGTG CAC^GTG
PmlI 1638 CACGTG CAC^GTG
RcaI 2732 TCATGA T^CATGA
SacI 1243 GAGCTC GAGCT^C
SexAI 3721 ACCAGGT A^CC[AT]GGT
SmaI 586 CCCGGG CCC^GGG
SpeI 3797 ACTAGT A^CTAGT
SphI 2615 GCATGC GCATG^C
Van91I 3256 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3256 CCAGTTGATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 586 CCCGGG C^CCGGG
XmaI 586 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 345 GTAGAC GT^[AC][GT]AC
393 GTAGAC
AhdI 462 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2473 GACCCCGTGTC
Aor51HI 1774 AGCGCT AGC^GCT
4510 AGCGCT
Asp700I 324 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3439 GAAGTCATTC
AspEI 462 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2473 GACCCCGTGTC
AspI 2712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4093 GACCCGGTC
AviII 102 TGCGCA TGC^GCA
211 TGCGCA
BamHI 70 GGATCC G^GATCC
4504 GGATCC
BbeI 193 GGCGCC GGCGC^C
590 GGCGCC
BcgI 1175 CGAAGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
105 GCAATGTTCTCG
BclI 2420 TGATCA T^GATCA
4185 TGATCA
BglII 256 AGATCT A^GATCT
652 AGATCT
BsaWI 3155 ACCGGA [AT]^CCGG[AT]
3773 TCCGGA
BsgI 1000 CTGCAC GTGCAG(16/14)
4059 GTGCAG
BsmBI 3101 GAGACG CGTCTC(1/5)
3277 GAGACG
BspMI 994 ACCTGC ACCTGC(4/8)
1311 GCAGGT
BsrFI 31 GCCGGC [GA]^CCGG[CT]
2151 GCCGGC
BstAPI 94 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1716 GCATTGGCTGC
BstXI 2651 CCACCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2765 CCAAAGAGCTGG
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
2151 GCCGGC
DrdI 3803 GACAGTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3822 GACCACTTGGTC
Eam1105I 462 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2473 GACCCCGTGTC
Eco47III 1774 AGCGCT AGC^GCT
4510 AGCGCT
FbaI 2420 TGATCA T^GATCA
4185 TGATCA
FspI 102 TGCGCA TGC^GCA
211 TGCGCA
HindIII 3222 AAGCTT A^AGCTT
3607 AAGCTT
KasI 193 GGCGCC G^GCGCC
590 GGCGCC
KspI 36 CCGCGG CCGC^GG
122 CCGCGG
NaeI 31 GCCGGC GCC^GGC
2151 GCCGGC
NarI 193 GGCGCC GG^CGCC
590 GGCGCC
NgoMIV 31 GCCGGC G^CCGGC
2151 GCCGGC
NspI 1653 ACATGT [GA]CATG^[CT]
2615 GCATGC
PflFI 2712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4093 GACCCGGTC
SacII 36 CCGCGG CCGC^GG
122 CCGCGG
SapI 924 GAAGAGC GCTCTTC(1/4)
1374 GCTCTTC
SfoI 193 GGCGCC GGC^GCC
590 GGCGCC
SmlI 1610 CTCAAG C^T[CT][GA]AG
4101 CTCAAG
TfiI 3373 GATTC G^A[AT]TC
3478 GAATC
Tth111I 2712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4093 GACCCGGTC
XmnI 324 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3439 GAAGTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrBI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII