################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccII AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BspDI BsrBI BsrDI BsrFI BssHII BstBI BstEII BstPI BstUI Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I KasI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 3604 GGTACC G^GTACC AccIII 157 TCCGGA T^CCGGA AflII 755 CTTAAG C^TTAAG Alw44I 2364 GTGCAC G^TGCAC ApaLI 2364 GTGCAC G^TGCAC Asp718I 3604 GGTACC G^GTACC BamHI 5390 GGATCC G^GATCC BanI 3604 GGTACC G^G[CT][GA]CC BfrI 755 CTTAAG C^TTAAG BsaI 5361 GAGACC GGTCTC(1/5) BsaWI 157 TCCGGA [AT]^CCGG[AT] BseAI 157 TCCGGA T^CCGGA BspEI 157 TCCGGA T^CCGGA BspHI 4120 TCATGA T^CATGA BspMI 1692 GCAGGT ACCTGC(4/8) BssSI 545 CACGAG CACGAG(-5/-1) Bst1107I 3316 GTATAC GTA^TAC BstAPI 3740 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3316 GTATAC GTA^TAC EcoNI 101 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 2138 GCGGG CCCGC(4/6) KpnI 3604 GGTACC GGTAC^C MroI 157 TCCGGA T^CCGGA NheI 1826 GCTAGC G^CTAGC PmeI 3289 GTTTAAAC GTTT^AAAC PpuMI 1721 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 4120 TCATGA T^CATGA SacI 5275 GAGCTC GAGCT^C SapI 5219 GAAGAGC GCTCTTC(1/4) SexAI 487 ACCAGGT A^CC[AT]GGT SnaBI 16 TACGTA TAC^GTA StuI 3831 AGGCCT AGG^CCT SwaI 3370 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AlwNI 2541 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 4920 CAGCTGCTG AvaI 2589 CTCGGG C^[CT]CG[GA]G 4133 CCCGAG BalI 88 TGGCCA TGG^CCA 4420 TGGCCA BanII 2684 GAGCCC G[GA]GC[CT]^C 5275 GAGCTC BbrPI 22 CACGTG CAC^GTG 4619 CACGTG BciVI 4684 GTATCC GTATCC(6/5) 5186 GTATCC BclI 468 TGATCA T^GATCA 4186 TGATCA BcnI 3424 CCGGG CC^[GC]GG 4497 CCGGG BglII 282 AGATCT A^GATCT 1297 AGATCT BsaBI 149 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 816 GATCTCCATC BseRI 2986 CTCCTC GAGGAG(10/8) 5249 GAGGAG BsmI 2905 GAATGC GAATGC(1/-1) 2052 GCATTC BsoBI 2589 CTCGGG C^[CT]CG[GA]G 4133 CCCGAG CfoI 961 GCGC GCG^C 4563 GCGC DraII 1721 AGGTCCC [GA]G^G[ACGT]CC[CT] 3488 AGGCCCT EcoO109I 1721 AGGTCCC [GA]G^G[ACGT]CC[CT] 3488 AGGCCCT FbaI 468 TGATCA T^GATCA 4186 TGATCA HhaI 961 GCGC GCG^C 4563 GCGC HinP1I 961 GCGC G^CGC 4563 GCGC HindIII 619 AAGCTT A^AGCTT 5501 AAGCTT HpaI 2698 GTTAAC GTT^AAC 4975 GTTAAC MamI 149 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 816 GATCTCCATC MluNI 88 TGGCCA TGG^CCA 4420 TGGCCA MscI 88 TGGCCA TGG^CCA 4420 TGGCCA NciI 3424 CCGGG CC^[GC]GG 4497 CCGGG PflMI 1004 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1763 CCACTGGATGG PmaCI 22 CACGTG CAC^GTG 4619 CACGTG PmlI 22 CACGTG CAC^GTG 4619 CACGTG PshAI 1595 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1734 GACACCTGTC ScaI 5620 AGTACT AGT^ACT 5691 AGTACT SphI 2925 GCATGC GCATG^C 5759 GCATGC SspI 1325 AATATT AAT^ATT 3170 AATATT TaqI 880 TCGA T^CGA 1603 TCGA TthHB8I 880 TCGA T^CGA 1603 TCGA Van91I 1004 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1763 CCACTGGATGG XcmI 4081 CCAAGCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5365 CCATTCAAACAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII