##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BspDI BsrBI
BsrDI BsrFI BssHII BstBI BstEII
BstPI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I KasI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3604 GGTACC G^GTACC
AccIII 157 TCCGGA T^CCGGA
AflII 755 CTTAAG C^TTAAG
Alw44I 2364 GTGCAC G^TGCAC
ApaLI 2364 GTGCAC G^TGCAC
Asp718I 3604 GGTACC G^GTACC
BamHI 5390 GGATCC G^GATCC
BanI 3604 GGTACC G^G[CT][GA]CC
BfrI 755 CTTAAG C^TTAAG
BsaI 5361 GAGACC GGTCTC(1/5)
BsaWI 157 TCCGGA [AT]^CCGG[AT]
BseAI 157 TCCGGA T^CCGGA
BspEI 157 TCCGGA T^CCGGA
BspHI 4120 TCATGA T^CATGA
BspMI 1692 GCAGGT ACCTGC(4/8)
BssSI 545 CACGAG CACGAG(-5/-1)
Bst1107I 3316 GTATAC GTA^TAC
BstAPI 3740 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3316 GTATAC GTA^TAC
EcoNI 101 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 2138 GCGGG CCCGC(4/6)
KpnI 3604 GGTACC GGTAC^C
MroI 157 TCCGGA T^CCGGA
NheI 1826 GCTAGC G^CTAGC
PmeI 3289 GTTTAAAC GTTT^AAAC
PpuMI 1721 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 4120 TCATGA T^CATGA
SacI 5275 GAGCTC GAGCT^C
SapI 5219 GAAGAGC GCTCTTC(1/4)
SexAI 487 ACCAGGT A^CC[AT]GGT
SnaBI 16 TACGTA TAC^GTA
StuI 3831 AGGCCT AGG^CCT
SwaI 3370 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwNI 2541 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
4920 CAGCTGCTG
AvaI 2589 CTCGGG C^[CT]CG[GA]G
4133 CCCGAG
BalI 88 TGGCCA TGG^CCA
4420 TGGCCA
BanII 2684 GAGCCC G[GA]GC[CT]^C
5275 GAGCTC
BbrPI 22 CACGTG CAC^GTG
4619 CACGTG
BciVI 4684 GTATCC GTATCC(6/5)
5186 GTATCC
BclI 468 TGATCA T^GATCA
4186 TGATCA
BcnI 3424 CCGGG CC^[GC]GG
4497 CCGGG
BglII 282 AGATCT A^GATCT
1297 AGATCT
BsaBI 149 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
816 GATCTCCATC
BseRI 2986 CTCCTC GAGGAG(10/8)
5249 GAGGAG
BsmI 2905 GAATGC GAATGC(1/-1)
2052 GCATTC
BsoBI 2589 CTCGGG C^[CT]CG[GA]G
4133 CCCGAG
CfoI 961 GCGC GCG^C
4563 GCGC
DraII 1721 AGGTCCC [GA]G^G[ACGT]CC[CT]
3488 AGGCCCT
EcoO109I 1721 AGGTCCC [GA]G^G[ACGT]CC[CT]
3488 AGGCCCT
FbaI 468 TGATCA T^GATCA
4186 TGATCA
HhaI 961 GCGC GCG^C
4563 GCGC
HinP1I 961 GCGC G^CGC
4563 GCGC
HindIII 619 AAGCTT A^AGCTT
5501 AAGCTT
HpaI 2698 GTTAAC GTT^AAC
4975 GTTAAC
MamI 149 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
816 GATCTCCATC
MluNI 88 TGGCCA TGG^CCA
4420 TGGCCA
MscI 88 TGGCCA TGG^CCA
4420 TGGCCA
NciI 3424 CCGGG CC^[GC]GG
4497 CCGGG
PflMI 1004 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCACTGGATGG
PmaCI 22 CACGTG CAC^GTG
4619 CACGTG
PmlI 22 CACGTG CAC^GTG
4619 CACGTG
PshAI 1595 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1734 GACACCTGTC
ScaI 5620 AGTACT AGT^ACT
5691 AGTACT
SphI 2925 GCATGC GCATG^C
5759 GCATGC
SspI 1325 AATATT AAT^ATT
3170 AATATT
TaqI 880 TCGA T^CGA
1603 TCGA
TthHB8I 880 TCGA T^CGA
1603 TCGA
Van91I 1004 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCACTGGATGG
XcmI 4081 CCAAGCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5365 CCATTCAAACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII