##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI BcgI BciVI BfrI
BseAI BspDI BspEI Bst1107I BstBI
BstZ17I Bsu36I ClaI DraI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI HpaI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI SalI
SfiI SfuI SgrAI SmlI SpeI
SphI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 881 GGTACC G^GTACC
Asp718I 881 GGTACC G^GTACC
AspI 3983 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3586 TGCGCA TGC^GCA
BamHI 491 GGATCC G^GATCC
BclI 1039 TGATCA T^GATCA
BsaBI 1553 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1663 CGTACG C^GTACG
BsmBI 3729 GAGACG CGTCTC(1/5)
BsmI 331 GCATTC GAATGC(1/-1)
BspHI 3010 TCATGA T^CATGA
BsrDI 1943 CATTGC GCAATG(2/0)
BstEII 3140 GGTCACC G^GT[ACGT]ACC
BstPI 3140 GGTCACC G^GT[ACGT]ACC
EcoO65I 3140 GGTCACC G^GT[ACGT]ACC
FbaI 1039 TGATCA T^GATCA
FspI 3586 TGCGCA TGC^GCA
HincII 3438 GTCAAC GT[CT]^[GA]AC
HindII 3438 GTCAAC GT[CT]^[GA]AC
KpnI 881 GGTACC GGTAC^C
KspI 554 CCGCGG CCGC^GG
MamI 1553 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2015 GCCGGC GCC^GGC
NgoMIV 2015 GCCGGC G^CCGGC
PflFI 3983 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3731 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3010 TCATGA T^CATGA
SacII 554 CCGCGG CCGC^GG
SapI 1505 GCTCTTC GCTCTTC(1/4)
SbfI 425 CCTGCAGG CCTGCA^GG
SexAI 4431 ACCTGGT A^CC[AT]GGT
SnaBI 2730 TACGTA TAC^GTA
Sse8387I 425 CCTGCAGG CCTGCA^GG
Tth111I 3983 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 577 AAATTT [GA]^AATT[CT]
3366 AAATTC
ApaI 56 GGGCCC GGGCC^C
949 GGGCCC
ApoI 577 AAATTT [GA]^AATT[CT]
3366 AAATTC
AvrII 1562 CCTAGG C^CTAGG
1727 CCTAGG
BbeI 782 GGCGCC GGCGC^C
2817 GGCGCC
BbrPI 1323 CACGTG CAC^GTG
3042 CACGTG
BglI 233 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1984 GCCTCAACGGC
BglII 916 AGATCT A^GATCT
4477 AGATCT
BlnI 1562 CCTAGG C^CTAGG
1727 CCTAGG
BsaWI 2560 ACCGGA [AT]^CCGG[AT]
4267 TCCGGT
Bsp1407I 1396 TGTACA T^GTACA
3208 TGTACA
BspLU11I 1399 ACATGT A^CATGT
3148 ACATGT
BsrBI 2250 GAGCGG CCGCTC(-3/-3)
2357 GAGCGG
BsrFI 1405 ACCGGC [GA]^CCGG[CT]
2015 GCCGGC
BsrGI 1396 TGTACA T^GTACA
3208 TGTACA
BstAPI 1894 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4407 GCAGAAAGTGC
Cfr10I 1405 ACCGGC [GA]^CCGG[CT]
2015 GCCGGC
CpoI 859 CGGACCG CG^G[AT]CCG
2182 CGGACCG
DraIII 2781 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3879 CACCAAGTG
DrdI 423 GACCTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
552 GACCGCGGGGTC
EagI 563 CGGCCG C^GGCCG
1996 CGGCCG
EclXI 563 CGGCCG C^GGCCG
1996 CGGCCG
Eco52I 563 CGGCCG C^GGCCG
1996 CGGCCG
KasI 782 GGCGCC G^GCGCC
2817 GGCGCC
NarI 782 GGCGCC GG^CGCC
2817 GGCGCC
PmaCI 1323 CACGTG CAC^GTG
3042 CACGTG
PmlI 1323 CACGTG CAC^GTG
3042 CACGTG
PstI 426 CTGCAG CTGCA^G
4586 CTGCAG
RsrII 859 CGGACCG CG^G[AT]CCG
2182 CGGACCG
SfoI 782 GGCGCC GGC^GCC
2817 GGCGCC
SmaI 140 CCCGGG CCC^GGG
390 CCCGGG
SspBI 1396 TGTACA T^GTACA
3208 TGTACA
SspI 4418 AATATT AAT^ATT
4524 AATATT
StuI 1225 AGGCCT AGG^CCT
3133 AGGCCT
XmaCI 140 CCCGGG C^CCGGG
390 CCCGGG
XmaI 140 CCCGGG C^CCGGG
390 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII