################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII Acc65I AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI BfrI BsiWI BsmBI BspDI BspLU11I BssHII Bst1107I BstAPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI KpnI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NsiI PacI PinAI PmeI PshAI PshBI PvuI RsrII SalI SbfI SgrAI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 2679 TCCGGA T^CCGGA AclI 4291 AACGTT AA^CGTT AflIII 4059 ACACGT A^C[GA][CT]GT AlwNI 4681 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2429 CCTGAGG CC^T[ACGT]AGG Aor51HI 2711 AGCGCT AGC^GCT AviII 1112 TGCGCA TGC^GCA AvrII 5053 CCTAGG C^CTAGG BbrPI 5210 CACGTG CAC^GTG BciVI 5012 GTATCC GTATCC(6/5) BlnI 5053 CCTAGG C^CTAGG BsaWI 2679 TCCGGA [AT]^CCGG[AT] BseAI 2679 TCCGGA T^CCGGA BsiEI 176 CGACCG CG[GA][CT]^CG BsmI 2065 GCATTC GAATGC(1/-1) BspEI 2679 TCCGGA T^CCGGA BspHI 3403 TCATGA T^CATGA BsrFI 1823 ACCGGC [GA]^CCGG[CT] BstBI 3059 TTCGAA TT^CGAA Bsu36I 2429 CCTGAGG CC^T[ACGT]AGG Cfr10I 1823 ACCGGC [GA]^CCGG[CT] DrdI 1548 GACATAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2711 AGCGCT AGC^GCT Eco81I 2429 CCTGAGG CC^T[ACGT]AGG FspI 1112 TGCGCA TGC^GCA KspI 84 CCGCGG CCGC^GG MroI 2679 TCCGGA T^CCGGA NspV 3059 TTCGAA TT^CGAA PaeR7I 1300 CTCGAG C^TCGAG PmaCI 5210 CACGTG CAC^GTG PmlI 5210 CACGTG CAC^GTG Psp1406I 4291 AACGTT AA^CGTT RcaI 3403 TCATGA T^CATGA SacII 84 CCGCGG CCGC^GG SfuI 3059 TTCGAA TT^CGAA SmaI 2077 CCCGGG CCC^GGG SspI 3275 AATATT AAT^ATT XbaI 735 TCTAGA T^CTAGA XhoI 1300 CTCGAG C^TCGAG XmaCI 2077 CCCGGG C^CCGGG XmaI 2077 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 2098 GGCGCC G[GA]^CG[CT]C 5091 GGCGCC Alw44I 1470 GTGCAC G^TGCAC 5207 GTGCAC ApaLI 1470 GTGCAC G^TGCAC 5207 GTGCAC Asp700I 1892 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2521 GAAGCTGTTC AspI 880 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3700 GACTTAGTC BamHI 1 GGATCC G^GATCC 1385 GGATCC BbeI 2098 GGCGCC GGCGC^C 5091 GGCGCC BclI 502 TGATCA T^GATCA 2467 TGATCA BglII 1427 AGATCT A^GATCT 4185 AGATCT BsaAI 2716 TACGTG [CT]AC^GT[GA] 5210 CACGTG BsaHI 2098 GGCGCC G[GA]^CG[CT]C 5091 GGCGCC Bsp1407I 1517 TGTACA T^GTACA 4056 TGTACA BsrDI 3439 CATTGC GCAATG(2/0) 2056 GCAATG BsrGI 1517 TGTACA T^GTACA 4056 TGTACA BstEII 5000 GGTCACC G^GT[ACGT]ACC 5411 GGTTACC BstPI 5000 GGTCACC G^GT[ACGT]ACC 5411 GGTTACC EcoO65I 5000 GGTCACC G^GT[ACGT]ACC 5411 GGTTACC EcoRI 828 GAATTC G^AATTC 4988 GAATTC FbaI 502 TGATCA T^GATCA 2467 TGATCA HgaI 2043 GACGC GACGC(5/10) 262 GCGTC Hin1I 2098 GGCGCC G[GA]^CG[CT]C 5091 GGCGCC HindIII 3883 AAGCTT A^AGCTT 4005 AAGCTT HpaI 2998 GTTAAC GTT^AAC 3722 GTTAAC KasI 2098 GGCGCC G^GCGCC 5091 GGCGCC NarI 2098 GGCGCC GG^CGCC 5091 GGCGCC NcoI 1230 CCATGG C^CATGG 1465 CCATGG PflFI 880 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3700 GACTTAGTC ScaI 1522 AGTACT AGT^ACT 4754 AGTACT SexAI 472 ACCTGGT A^CC[AT]GGT 5415 ACCAGGT SfiI 5048 GGCCTCCTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5532 GGCCTGCTGGGCC SfoI 2098 GGCGCC GGC^GCC 5091 GGCGCC SspBI 1517 TGTACA T^GTACA 4056 TGTACA Tth111I 880 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3700 GACTTAGTC XcmI 1846 CCAGCTCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4805 CCAACAGCATGATGG XmnI 1892 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2521 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII