##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BfrI BsiWI BsmBI BspDI BspLU11I
BssHII Bst1107I BstAPI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI KpnI
MfeI MluI MunI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PinAI PmeI PshAI PshBI PvuI
RsrII SalI SbfI SgrAI SnaBI
SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2679 TCCGGA T^CCGGA
AclI 4291 AACGTT AA^CGTT
AflIII 4059 ACACGT A^C[GA][CT]GT
AlwNI 4681 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2429 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2711 AGCGCT AGC^GCT
AviII 1112 TGCGCA TGC^GCA
AvrII 5053 CCTAGG C^CTAGG
BbrPI 5210 CACGTG CAC^GTG
BciVI 5012 GTATCC GTATCC(6/5)
BlnI 5053 CCTAGG C^CTAGG
BsaWI 2679 TCCGGA [AT]^CCGG[AT]
BseAI 2679 TCCGGA T^CCGGA
BsiEI 176 CGACCG CG[GA][CT]^CG
BsmI 2065 GCATTC GAATGC(1/-1)
BspEI 2679 TCCGGA T^CCGGA
BspHI 3403 TCATGA T^CATGA
BsrFI 1823 ACCGGC [GA]^CCGG[CT]
BstBI 3059 TTCGAA TT^CGAA
Bsu36I 2429 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1823 ACCGGC [GA]^CCGG[CT]
DrdI 1548 GACATAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2711 AGCGCT AGC^GCT
Eco81I 2429 CCTGAGG CC^T[ACGT]AGG
FspI 1112 TGCGCA TGC^GCA
KspI 84 CCGCGG CCGC^GG
MroI 2679 TCCGGA T^CCGGA
NspV 3059 TTCGAA TT^CGAA
PaeR7I 1300 CTCGAG C^TCGAG
PmaCI 5210 CACGTG CAC^GTG
PmlI 5210 CACGTG CAC^GTG
Psp1406I 4291 AACGTT AA^CGTT
RcaI 3403 TCATGA T^CATGA
SacII 84 CCGCGG CCGC^GG
SfuI 3059 TTCGAA TT^CGAA
SmaI 2077 CCCGGG CCC^GGG
SspI 3275 AATATT AAT^ATT
XbaI 735 TCTAGA T^CTAGA
XhoI 1300 CTCGAG C^TCGAG
XmaCI 2077 CCCGGG C^CCGGG
XmaI 2077 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 2098 GGCGCC G[GA]^CG[CT]C
5091 GGCGCC
Alw44I 1470 GTGCAC G^TGCAC
5207 GTGCAC
ApaLI 1470 GTGCAC G^TGCAC
5207 GTGCAC
Asp700I 1892 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2521 GAAGCTGTTC
AspI 880 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3700 GACTTAGTC
BamHI 1 GGATCC G^GATCC
1385 GGATCC
BbeI 2098 GGCGCC GGCGC^C
5091 GGCGCC
BclI 502 TGATCA T^GATCA
2467 TGATCA
BglII 1427 AGATCT A^GATCT
4185 AGATCT
BsaAI 2716 TACGTG [CT]AC^GT[GA]
5210 CACGTG
BsaHI 2098 GGCGCC G[GA]^CG[CT]C
5091 GGCGCC
Bsp1407I 1517 TGTACA T^GTACA
4056 TGTACA
BsrDI 3439 CATTGC GCAATG(2/0)
2056 GCAATG
BsrGI 1517 TGTACA T^GTACA
4056 TGTACA
BstEII 5000 GGTCACC G^GT[ACGT]ACC
5411 GGTTACC
BstPI 5000 GGTCACC G^GT[ACGT]ACC
5411 GGTTACC
EcoO65I 5000 GGTCACC G^GT[ACGT]ACC
5411 GGTTACC
EcoRI 828 GAATTC G^AATTC
4988 GAATTC
FbaI 502 TGATCA T^GATCA
2467 TGATCA
HgaI 2043 GACGC GACGC(5/10)
262 GCGTC
Hin1I 2098 GGCGCC G[GA]^CG[CT]C
5091 GGCGCC
HindIII 3883 AAGCTT A^AGCTT
4005 AAGCTT
HpaI 2998 GTTAAC GTT^AAC
3722 GTTAAC
KasI 2098 GGCGCC G^GCGCC
5091 GGCGCC
NarI 2098 GGCGCC GG^CGCC
5091 GGCGCC
NcoI 1230 CCATGG C^CATGG
1465 CCATGG
PflFI 880 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3700 GACTTAGTC
ScaI 1522 AGTACT AGT^ACT
4754 AGTACT
SexAI 472 ACCTGGT A^CC[AT]GGT
5415 ACCAGGT
SfiI 5048 GGCCTCCTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5532 GGCCTGCTGGGCC
SfoI 2098 GGCGCC GGC^GCC
5091 GGCGCC
SspBI 1517 TGTACA T^GTACA
4056 TGTACA
Tth111I 880 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3700 GACTTAGTC
XcmI 1846 CCAGCTCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4805 CCAACAGCATGATGG
XmnI 1892 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2521 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII