##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AviII BbeI BbrPI BcgI BciVI
BfrI BsaHI BsiWI BsmBI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI Hin1I
HpaI KasI KspI MluI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 380 CGCG CG^CG
AccIII 2157 TCCGGA T^CCGGA
AflIII 2857 ACACGT A^C[GA][CT]GT
AspI 1085 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1471 TGGCCA TGG^CCA
BamHI 1871 GGATCC G^GATCC
BbvCI 2446 CCTCAGC CCTCAGC(-5/-2)
BlpI 3635 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3635 GCTTAGC GC^T[ACGT]AGC
BseAI 2157 TCCGGA T^CCGGA
BsiEI 1197 CGGTCG CG[GA][CT]^CG
Bsp1407I 4422 TGTACA T^GTACA
BspEI 2157 TCCGGA T^CCGGA
BsrFI 2046 GCCGGT [GA]^CCGG[CT]
BsrGI 4422 TGTACA T^GTACA
BssSI 3276 CACGAG CACGAG(-5/-1)
BstEII 5548 GGTGACC G^GT[ACGT]ACC
BstPI 5548 GGTGACC G^GT[ACGT]ACC
BstUI 380 CGCG CG^CG
BstXI 2686 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3635 GCTTAGC GC^T[ACGT]AGC
Cfr10I 2046 GCCGGT [GA]^CCGG[CT]
DraIII 3520 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 5548 GGTGACC G^GT[ACGT]ACC
HgaI 3734 GACGC GACGC(5/10)
HincII 6088 GTCAAC GT[CT]^[GA]AC
HindII 6088 GTCAAC GT[CT]^[GA]AC
MfeI 4007 CAATTG C^AATTG
MluNI 1471 TGGCCA TGG^CCA
MroI 2157 TCCGGA T^CCGGA
MscI 1471 TGGCCA TGG^CCA
MunI 4007 CAATTG C^AATTG
MvnI 380 CGCG CG^CG
PaeR7I 1699 CTCGAG C^TCGAG
PflFI 1085 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 405 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 2619 AGTACT AGT^ACT
SmaI 309 CCCGGG CCC^GGG
SspBI 4422 TGTACA T^GTACA
SspI 1165 AATATT AAT^ATT
Tth111I 1085 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1699 CTCGAG C^TCGAG
XmaCI 309 CCCGGG C^CCGGG
XmaI 309 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 261 GGTACC G^GTACC
3697 GGTACC
AccI 1106 GTCTAC GT^[AC][GT]AC
5448 GTAGAC
AhdI 1357 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2818 GACCCCTGGTC
Asp718I 261 GGTACC G^GTACC
3697 GGTACC
AspEI 1357 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2818 GACCCCTGGTC
BclI 1489 TGATCA T^GATCA
1992 TGATCA
BglI 628 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2538 GCCTGGCTGGC
BglII 3466 AGATCT A^GATCT
4987 AGATCT
BsaAI 2475 CACGTA [CT]AC^GT[GA]
2858 CACGTA
BsaBI 3026 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5866 GATTTATATC
BsmI 3930 GCATTC GAATGC(1/-1)
4883 GCATTC
BsrBI 420 CCGCTC CCGCTC(-3/-3)
1451 GAGCGG
EaeI 315 CGGCCA [CT]^GGCC[GA]
1471 TGGCCA
Eam1105I 1357 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2818 GACCCCTGGTC
EcoNI 397 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3649 CCTGTTTAAGG
EcoRI 689 GAATTC G^AATTC
5405 GAATTC
EcoRV 2780 GATATC GAT^ATC
3221 GATATC
FbaI 1489 TGATCA T^GATCA
1992 TGATCA
HaeII 166 GGCGCT [GA]GCGC^[CT]
4458 AGCGCC
KpnI 261 GGTACC GGTAC^C
3697 GGTACC
MamI 3026 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5866 GATTTATATC
PflMI 265 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1441 CCAGAATTTGG
PshAI 1856 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5979 GACAGTTGTC
PvuII 71 CAGCTG CAG^CTG
538 CAGCTG
SacI 1652 GAGCTC GAGCT^C
2746 GAGCTC
SexAI 678 ACCTGGT A^CC[AT]GGT
6171 ACCTGGT
SphI 1095 GCATGC GCATG^C
6012 GCATGC
StuI 817 AGGCCT AGG^CCT
2149 AGGCCT
Van91I 265 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1441 CCAGAATTTGG
XbaI 445 TCTAGA T^CTAGA
4870 TCTAGA
XcmI 3614 CCAGATCAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6162 CCATGGAATACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII AvrII
BanI BanII BbsI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI