################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AviII BbeI BbrPI BcgI BciVI BfrI BsaHI BsiWI BsmBI BspDI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I FseI FspI Hin1I HpaI KasI KspI MluI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 380 CGCG CG^CG AccIII 2157 TCCGGA T^CCGGA AflIII 2857 ACACGT A^C[GA][CT]GT AspI 1085 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1471 TGGCCA TGG^CCA BamHI 1871 GGATCC G^GATCC BbvCI 2446 CCTCAGC CCTCAGC(-5/-2) BlpI 3635 GCTTAGC GC^T[ACGT]AGC Bpu1102I 3635 GCTTAGC GC^T[ACGT]AGC BseAI 2157 TCCGGA T^CCGGA BsiEI 1197 CGGTCG CG[GA][CT]^CG Bsp1407I 4422 TGTACA T^GTACA BspEI 2157 TCCGGA T^CCGGA BsrFI 2046 GCCGGT [GA]^CCGG[CT] BsrGI 4422 TGTACA T^GTACA BssSI 3276 CACGAG CACGAG(-5/-1) BstEII 5548 GGTGACC G^GT[ACGT]ACC BstPI 5548 GGTGACC G^GT[ACGT]ACC BstUI 380 CGCG CG^CG BstXI 2686 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 3635 GCTTAGC GC^T[ACGT]AGC Cfr10I 2046 GCCGGT [GA]^CCGG[CT] DraIII 3520 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 5548 GGTGACC G^GT[ACGT]ACC HgaI 3734 GACGC GACGC(5/10) HincII 6088 GTCAAC GT[CT]^[GA]AC HindII 6088 GTCAAC GT[CT]^[GA]AC MfeI 4007 CAATTG C^AATTG MluNI 1471 TGGCCA TGG^CCA MroI 2157 TCCGGA T^CCGGA MscI 1471 TGGCCA TGG^CCA MunI 4007 CAATTG C^AATTG MvnI 380 CGCG CG^CG PaeR7I 1699 CTCGAG C^TCGAG PflFI 1085 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 405 AGGACCT [GA]G^G[AT]CC[CT] ScaI 2619 AGTACT AGT^ACT SmaI 309 CCCGGG CCC^GGG SspBI 4422 TGTACA T^GTACA SspI 1165 AATATT AAT^ATT Tth111I 1085 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1699 CTCGAG C^TCGAG XmaCI 309 CCCGGG C^CCGGG XmaI 309 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 261 GGTACC G^GTACC 3697 GGTACC AccI 1106 GTCTAC GT^[AC][GT]AC 5448 GTAGAC AhdI 1357 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2818 GACCCCTGGTC Asp718I 261 GGTACC G^GTACC 3697 GGTACC AspEI 1357 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2818 GACCCCTGGTC BclI 1489 TGATCA T^GATCA 1992 TGATCA BglI 628 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2538 GCCTGGCTGGC BglII 3466 AGATCT A^GATCT 4987 AGATCT BsaAI 2475 CACGTA [CT]AC^GT[GA] 2858 CACGTA BsaBI 3026 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5866 GATTTATATC BsmI 3930 GCATTC GAATGC(1/-1) 4883 GCATTC BsrBI 420 CCGCTC CCGCTC(-3/-3) 1451 GAGCGG EaeI 315 CGGCCA [CT]^GGCC[GA] 1471 TGGCCA Eam1105I 1357 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2818 GACCCCTGGTC EcoNI 397 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3649 CCTGTTTAAGG EcoRI 689 GAATTC G^AATTC 5405 GAATTC EcoRV 2780 GATATC GAT^ATC 3221 GATATC FbaI 1489 TGATCA T^GATCA 1992 TGATCA HaeII 166 GGCGCT [GA]GCGC^[CT] 4458 AGCGCC KpnI 261 GGTACC GGTAC^C 3697 GGTACC MamI 3026 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5866 GATTTATATC PflMI 265 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1441 CCAGAATTTGG PshAI 1856 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5979 GACAGTTGTC PvuII 71 CAGCTG CAG^CTG 538 CAGCTG SacI 1652 GAGCTC GAGCT^C 2746 GAGCTC SexAI 678 ACCTGGT A^CC[AT]GGT 6171 ACCTGGT SphI 1095 GCATGC GCATG^C 6012 GCATGC StuI 817 AGGCCT AGG^CCT 2149 AGGCCT Van91I 265 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1441 CCAGAATTTGG XbaI 445 TCTAGA T^CTAGA 4870 TCTAGA XcmI 3614 CCAGATCAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6162 CCATGGAATACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AvaI AvaII AvrII BanI BanII BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI