##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AccIII      AclI        AhdI        Aor51HI     
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbrPI       BclI        BlnI        BsaBI       
BsaI        BseAI       BsiWI       BsmI        BspDI       
BspEI       BssHII      BstBI       ClaI        CpoI        
DrdI        Eam1105I    Eco47III    EcoRV       EcoT22I     
FbaI        FseI        FspI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NotI        
NsiI        NspV        PacI        PmaCI       PmeI        
PmlI        PshBI       Psp1406I    RsrII       SalI        
SbfI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    StuI        SwaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       2294  GGTACC               G^GTACC
AccI         4999  GTATAC               GT^[AC][GT]AC
AgeI         3115  ACCGGT               A^CCGGT
AocI         1464  CCTGAGG              CC^T[ACGT]AGG
ApaI          620  GGGCCC               GGGCC^C
Asp718I      2294  GGTACC               G^GTACC
AspI          600  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI          39  GGATCC               G^GATCC
BbvCI         384  CCTCAGC              CCTCAGC(-5/-2)
BcgI         3050  CGAGTCCGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1640  AGATCT               A^GATCT
BlpI           15  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       15  GCTGAGC              GC^T[ACGT]AGC
BsaAI        4258  CACGTA               [CT]AC^GT[GA]
BsaWI        3115  ACCGGT               [AT]^CCGG[AT]
BsmBI        3846  CGTCTC               CGTCTC(1/5)
BspHI         596  TCATGA               T^CATGA
BsrDI        5007  GCAATG               GCAATG(2/0)
BssSI        3986  CTCGTG               CACGAG(-5/-1)
Bst1107I     4999  GTATAC               GTA^TAC
BstEII       2142  GGTTACC              G^GT[ACGT]ACC
BstPI        2142  GGTTACC              G^GT[ACGT]ACC
BstXI        4949  CCATCATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      4999  GTATAC               GTA^TAC
Bsu36I       1464  CCTGAGG              CC^T[ACGT]AGG
CelII          15  GCTGAGC              GC^T[ACGT]AGC
DraIII       1587  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1464  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2142  GGTTACC              G^GT[ACGT]ACC
EcoRI        1846  GAATTC               G^AATTC
HindIII       292  AAGCTT               A^AGCTT
HpaI         4297  GTTAAC               GTT^AAC
KpnI         2294  GGTACC               GGTAC^C
KspI         5322  CCGCGG               CCGC^GG
NcoI          938  CCATGG               C^CATGG
NruI         2389  TCGCGA               TCG^CGA
PaeR7I       4485  CTCGAG               C^TCGAG
PflFI         600  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3115  ACCGGT               A^CCGGT
PshAI         996  GACTGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         3916  CGATCG               CGAT^CG
RcaI          596  TCATGA               T^CATGA
SacI          565  GAGCTC               GAGCT^C
SacII        5322  CCGCGG               CCGC^GG
SapI         1470  GAAGAGC              GCTCTTC(1/4)
ScaI         4138  AGTACT               AGT^ACT
SmaI         2486  CCCGGG               CCC^GGG
SphI           11  GCATGC               GCATG^C
SspI         5445  AATATT               AAT^ATT
Tth111I       600  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         5215  TCTAGA               T^CTAGA
XhoI         4485  CTCGAG               C^TCGAG
XmaCI        2486  CCCGGG               C^CCGGG
XmaI         2486  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflII        3945  CTTAAG               C^TTAAG
             5111  CTTAAG              
Alw44I        275  GTGCAC               G^TGCAC
             1370  GTGCAC              
ApaLI         275  GTGCAC               G^TGCAC
             1370  GTGCAC              
BbeI         1234  GGCGCC               GGCGC^C
             3166  GGCGCC              
BciVI        3685  GGATAC               GTATCC(6/5)
             3213  GTATCC              
BfrI         3945  CTTAAG               C^TTAAG
             5111  CTTAAG              
Bsp1407I     2258  TGTACA               T^GTACA
             5366  TGTACA              
BsrGI        2258  TGTACA               T^GTACA
             5366  TGTACA              
EagI         1613  CGGCCG               C^GGCCG
             1992  CGGCCG              
EclXI        1613  CGGCCG               C^GGCCG
             1992  CGGCCG              
Eco52I       1613  CGGCCG               C^GGCCG
             1992  CGGCCG              
HaeII        1234  GGCGCC               [GA]GCGC^[CT]
             3166  GGCGCC              
HincII       1573  GTCAAC               GT[CT]^[GA]AC
             4297  GTTAAC              
HindII       1573  GTCAAC               GT[CT]^[GA]AC
             4297  GTTAAC              
KasI         1234  GGCGCC               G^GCGCC
             3166  GGCGCC              
MslI         3378  CATAAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4819  CATAGTCATG          
NarI         1234  GGCGCC               GG^CGCC
             3166  GGCGCC              
NheI         4599  GCTAGC               G^CTAGC
             4850  GCTAGC              
PpuMI        2649  GGGTCCC              [GA]G^G[AT]CC[CT]
             4765  AGGACCC             
SexAI         515  ACCTGGT              A^CC[AT]GGT
             1949  ACCTGGT             
SfoI         1234  GGCGCC               GGC^GCC
             3166  GGCGCC              
SspBI        2258  TGTACA               T^GTACA
             5366  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspLU11I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI