##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BbrPI BclI BlnI BsaBI
BsaI BseAI BsiWI BsmI BspDI
BspEI BssHII BstBI ClaI CpoI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI FspI MamI MfeI
MluI MroI MunI NdeI NotI
NsiI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2294 GGTACC G^GTACC
AccI 4999 GTATAC GT^[AC][GT]AC
AgeI 3115 ACCGGT A^CCGGT
AocI 1464 CCTGAGG CC^T[ACGT]AGG
ApaI 620 GGGCCC GGGCC^C
Asp718I 2294 GGTACC G^GTACC
AspI 600 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 39 GGATCC G^GATCC
BbvCI 384 CCTCAGC CCTCAGC(-5/-2)
BcgI 3050 CGAGTCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1640 AGATCT A^GATCT
BlpI 15 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
BsaAI 4258 CACGTA [CT]AC^GT[GA]
BsaWI 3115 ACCGGT [AT]^CCGG[AT]
BsmBI 3846 CGTCTC CGTCTC(1/5)
BspHI 596 TCATGA T^CATGA
BsrDI 5007 GCAATG GCAATG(2/0)
BssSI 3986 CTCGTG CACGAG(-5/-1)
Bst1107I 4999 GTATAC GTA^TAC
BstEII 2142 GGTTACC G^GT[ACGT]ACC
BstPI 2142 GGTTACC G^GT[ACGT]ACC
BstXI 4949 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4999 GTATAC GTA^TAC
Bsu36I 1464 CCTGAGG CC^T[ACGT]AGG
CelII 15 GCTGAGC GC^T[ACGT]AGC
DraIII 1587 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1464 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2142 GGTTACC G^GT[ACGT]ACC
EcoRI 1846 GAATTC G^AATTC
HindIII 292 AAGCTT A^AGCTT
HpaI 4297 GTTAAC GTT^AAC
KpnI 2294 GGTACC GGTAC^C
KspI 5322 CCGCGG CCGC^GG
NcoI 938 CCATGG C^CATGG
NruI 2389 TCGCGA TCG^CGA
PaeR7I 4485 CTCGAG C^TCGAG
PflFI 600 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3115 ACCGGT A^CCGGT
PshAI 996 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3916 CGATCG CGAT^CG
RcaI 596 TCATGA T^CATGA
SacI 565 GAGCTC GAGCT^C
SacII 5322 CCGCGG CCGC^GG
SapI 1470 GAAGAGC GCTCTTC(1/4)
ScaI 4138 AGTACT AGT^ACT
SmaI 2486 CCCGGG CCC^GGG
SphI 11 GCATGC GCATG^C
SspI 5445 AATATT AAT^ATT
Tth111I 600 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5215 TCTAGA T^CTAGA
XhoI 4485 CTCGAG C^TCGAG
XmaCI 2486 CCCGGG C^CCGGG
XmaI 2486 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 3945 CTTAAG C^TTAAG
5111 CTTAAG
Alw44I 275 GTGCAC G^TGCAC
1370 GTGCAC
ApaLI 275 GTGCAC G^TGCAC
1370 GTGCAC
BbeI 1234 GGCGCC GGCGC^C
3166 GGCGCC
BciVI 3685 GGATAC GTATCC(6/5)
3213 GTATCC
BfrI 3945 CTTAAG C^TTAAG
5111 CTTAAG
Bsp1407I 2258 TGTACA T^GTACA
5366 TGTACA
BsrGI 2258 TGTACA T^GTACA
5366 TGTACA
EagI 1613 CGGCCG C^GGCCG
1992 CGGCCG
EclXI 1613 CGGCCG C^GGCCG
1992 CGGCCG
Eco52I 1613 CGGCCG C^GGCCG
1992 CGGCCG
HaeII 1234 GGCGCC [GA]GCGC^[CT]
3166 GGCGCC
HincII 1573 GTCAAC GT[CT]^[GA]AC
4297 GTTAAC
HindII 1573 GTCAAC GT[CT]^[GA]AC
4297 GTTAAC
KasI 1234 GGCGCC G^GCGCC
3166 GGCGCC
MslI 3378 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4819 CATAGTCATG
NarI 1234 GGCGCC GG^CGCC
3166 GGCGCC
NheI 4599 GCTAGC G^CTAGC
4850 GCTAGC
PpuMI 2649 GGGTCCC [GA]G^G[AT]CC[CT]
4765 AGGACCC
SexAI 515 ACCTGGT A^CC[AT]GGT
1949 ACCTGGT
SfoI 1234 GGCGCC GGC^GCC
3166 GGCGCC
SspBI 2258 TGTACA T^GTACA
5366 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI