##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AocI Aor51HI AscI AseI
AsnI AviII AvrII BbeI BlnI
BspDI BspHI BsrDI BssHII BstAPI
BstBI Bsu36I ClaI CpoI DraI
Eco47III Eco81I EcoRV EcoT22I FseI
FspI HpaI KasI MluI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SfiI
SfoI SfuI SnaBI SpeI SphI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 1204 TCCGGA T^CCGGA
AflII 1926 CTTAAG C^TTAAG
AhdI 3593 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3106 GTGCAC G^TGCAC
ApaLI 3106 GTGCAC G^TGCAC
Asp700I 3817 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3593 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 751 GGATCC G^GATCC
BbrPI 2259 CACGTG CAC^GTG
BfrI 1926 CTTAAG C^TTAAG
BglII 2020 AGATCT A^GATCT
BseAI 1204 TCCGGA T^CCGGA
Bsp1407I 2653 TGTACA T^GTACA
BspEI 1204 TCCGGA T^CCGGA
BspLU11I 3715 ACATGT A^CATGT
BspMI 4196 GCAGGT ACCTGC(4/8)
BsrBI 3937 CCGCTC CCGCTC(-3/-3)
BsrGI 2653 TGTACA T^GTACA
Eam1105I 3593 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 725 GAATTC G^AATTC
HaeII 2536 AGCGCC [GA]GCGC^[CT]
HincII 1215 GTCAAC GT[CT]^[GA]AC
HindII 1215 GTCAAC GT[CT]^[GA]AC
HindIII 1561 AAGCTT A^AGCTT
MfeI 157 CAATTG C^AATTG
MroI 1204 TCCGGA T^CCGGA
MunI 157 CAATTG C^AATTG
PaeR7I 2203 CTCGAG C^TCGAG
PmaCI 2259 CACGTG CAC^GTG
PmlI 2259 CACGTG CAC^GTG
PshAI 2882 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1359 GAGCTC GAGCT^C
SbfI 908 CCTGCAGG CCTGCA^GG
ScaI 131 AGTACT AGT^ACT
SgrAI 207 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 775 CCCGGG CCC^GGG
Sse8387I 908 CCTGCAGG CCTGCA^GG
SspBI 2653 TGTACA T^GTACA
TfiI 282 GAATC G^A[AT]TC
XhoI 2203 CTCGAG C^TCGAG
XmaCI 775 CCCGGG C^CCGGG
XmaI 775 CCCGGG C^CCGGG
XmnI 3817 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2160 GACGTC GACGT^C
3165 GACGTC
AcsI 441 GAATTT [GA]^AATT[CT]
725 GAATTC
AgeI 1408 ACCGGT A^CCGGT
2806 ACCGGT
ApoI 441 GAATTT [GA]^AATT[CT]
725 GAATTC
BbsI 1370 GAAGAC GAAGAC(2/6)
2108 GAAGAC
BcgI 391 GCACTGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4277 GCAGGAGGATCG
BciVI 903 GTATCC GTATCC(6/5)
3498 GTATCC
BglI 500 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1995 GCCCAGACGGC
BpuAI 1370 GAAGAC GAAGAC(2/6)
2108 GAAGAC
BsaAI 430 TACGTG [CT]AC^GT[GA]
2259 CACGTG
BsaBI 766 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1863 GATGATCATC
BsiWI 1852 CGTACG C^GTACG
2360 CGTACG
BsmBI 1148 CGTCTC CGTCTC(1/5)
1800 GAGACG
BsmI 1698 GAATGC GAATGC(1/-1)
3303 GCATTC
BssSI 1608 CACGAG CACGAG(-5/-1)
975 CTCGTG
Bst1107I 108 GTATAC GTA^TAC
1329 GTATAC
BstEII 2276 GGTGACC G^GT[ACGT]ACC
3038 GGTGACC
BstPI 2276 GGTGACC G^GT[ACGT]ACC
3038 GGTGACC
BstXI 1384 CCATTGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3332 CCAGCCAGCTGG
BstZ17I 108 GTATAC GTA^TAC
1329 GTATAC
CfoI 1897 GCGC GCG^C
2537 GCGC
DraIII 186 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1495 CACCCAGTG
DrdI 214 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3386 GACACAGGTGTC
EcoO65I 2276 GGTGACC G^GT[ACGT]ACC
3038 GGTGACC
HhaI 1897 GCGC GCG^C
2537 GCGC
HinP1I 1897 GCGC G^CGC
2537 GCGC
MamI 766 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1863 GATGATCATC
NaeI 208 GCCGGC GCC^GGC
2853 GCCGGC
NgoMIV 208 GCCGGC G^CCGGC
2853 GCCGGC
PinAI 1408 ACCGGT A^CCGGT
2806 ACCGGT
SmlI 1926 CTTAAG C^T[CT][GA]AG
2203 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI Asp718I AspHI AspI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI KpnI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII