##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AcsI ApoI AscI AvrII
BcgI BclI BlnI BsaBI BssHII
Bst1107I BstZ17I CpoI EcoNI EcoRI
FbaI FseI HpaI MamI MfeI
MluI MunI NdeI NotI NruI
PacI PaeR7I PmeI PvuI RsrII
SalI SapI SbfI ScaI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 4282 AACGTT AA^CGTT
AflII 4888 CTTAAG C^TTAAG
AgeI 3818 ACCGGT A^CCGGT
AhdI 1518 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1763 ATTAAT AT^TAAT
AsnI 1763 ATTAAT AT^TAAT
Asp700I 947 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1518 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 845 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2193 TGCGCA TGC^GCA
BfrI 4888 CTTAAG C^TTAAG
BsiWI 291 CGTACG C^GTACG
BsmBI 3918 CGTCTC CGTCTC(1/5)
Bsp1407I 4478 TGTACA T^GTACA
BspMI 234 ACCTGC ACCTGC(4/8)
BsrGI 4478 TGTACA T^GTACA
BssSI 3501 CTCGTG CACGAG(-5/-1)
BstBI 1182 TTCGAA TT^CGAA
DraI 1342 TTTAAA TTT^AAA
DrdI 5281 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 501 CGGCCG C^GGCCG
Eam1105I 1518 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 501 CGGCCG C^GGCCG
Eco52I 501 CGGCCG C^GGCCG
FspI 2193 TGCGCA TGC^GCA
HgaI 3448 GACGC GACGC(5/10)
HincII 3370 GTCAAC GT[CT]^[GA]AC
HindII 3370 GTCAAC GT[CT]^[GA]AC
HindIII 3770 AAGCTT A^AGCTT
NspV 1182 TTCGAA TT^CGAA
PflFI 845 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3818 ACCGGT A^CCGGT
PpuMI 5279 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 4366 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1763 ATTAAT AT^TAAT
Psp1406I 4282 AACGTT AA^CGTT
SacI 3089 GAGCTC GAGCT^C
SexAI 846 ACCAGGT A^CC[AT]GGT
SfiI 214 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1182 TTCGAA TT^CGAA
SphI 4497 GCATGC GCATG^C
SspBI 4478 TGTACA T^GTACA
Tth111I 845 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 947 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2425 GGTACC G^GTACC
4632 GGTACC
AccI 350 GTCTAC GT^[AC][GT]AC
3693 GTCTAC
AccIII 834 TCCGGA T^CCGGA
3356 TCCGGA
AcyI 1019 GGCGCC G[GA]^CG[CT]C
2715 GGCGCC
Alw44I 3269 GTGCAC G^TGCAC
3307 GTGCAC
AocI 3723 CCTAAGG CC^T[ACGT]AGG
3796 CCTGAGG
Aor51HI 309 AGCGCT AGC^GCT
3612 AGCGCT
ApaI 222 GGGCCC GGGCC^C
2769 GGGCCC
ApaLI 3269 GTGCAC G^TGCAC
3307 GTGCAC
Asp718I 2425 GGTACC G^GTACC
4632 GGTACC
BbeI 1019 GGCGCC GGCGC^C
2715 GGCGCC
BbrPI 806 CACGTG CAC^GTG
4872 CACGTG
BglII 381 AGATCT A^GATCT
1800 AGATCT
BsaHI 1019 GGCGCC G[GA]^CG[CT]C
2715 GGCGCC
BsaI 3506 GAGACC GGTCTC(1/5)
4701 GGTCTC
BseAI 834 TCCGGA T^CCGGA
3356 TCCGGA
BsiEI 459 CGGTCG CG[GA][CT]^CG
501 CGGCCG
BsmI 2603 GCATTC GAATGC(1/-1)
5165 GCATTC
BspDI 744 ATCGAT AT^CGAT
1049 ATCGAT
BspEI 834 TCCGGA T^CCGGA
3356 TCCGGA
BspHI 2314 TCATGA T^CATGA
5121 TCATGA
BstEII 2358 GGTTACC G^GT[ACGT]ACC
4412 GGTCACC
BstPI 2358 GGTTACC G^GT[ACGT]ACC
4412 GGTCACC
Bsu36I 3723 CCTAAGG CC^T[ACGT]AGG
3796 CCTGAGG
ClaI 744 ATCGAT AT^CGAT
1049 ATCGAT
DraIII 2411 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2979 CACTTTGTG
Eco47III 309 AGCGCT AGC^GCT
3612 AGCGCT
Eco81I 3723 CCTAAGG CC^T[ACGT]AGG
3796 CCTGAGG
EcoO65I 2358 GGTTACC G^GT[ACGT]ACC
4412 GGTCACC
EcoRV 1412 GATATC GAT^ATC
3293 GATATC
EcoT22I 2601 ATGCAT ATGCA^T
4820 ATGCAT
Hin1I 1019 GGCGCC G[GA]^CG[CT]C
2715 GGCGCC
KasI 1019 GGCGCC G^GCGCC
2715 GGCGCC
KpnI 2425 GGTACC GGTAC^C
4632 GGTACC
KspI 157 CCGCGG CCGC^GG
365 CCGCGG
MroI 834 TCCGGA T^CCGGA
3356 TCCGGA
NarI 1019 GGCGCC GG^CGCC
2715 GGCGCC
NcoI 696 CCATGG C^CATGG
1654 CCATGG
NheI 2906 GCTAGC G^CTAGC
4803 GCTAGC
NsiI 2601 ATGCAT ATGCA^T
4820 ATGCAT
PmaCI 806 CACGTG CAC^GTG
4872 CACGTG
PmlI 806 CACGTG CAC^GTG
4872 CACGTG
RcaI 2314 TCATGA T^CATGA
5121 TCATGA
SacII 157 CCGCGG CCGC^GG
365 CCGCGG
SfoI 1019 GGCGCC GGC^GCC
2715 GGCGCC
StuI 2098 AGGCCT AGG^CCT
3325 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI