################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AatII AcsI ApoI AscI AvrII BcgI BclI BlnI BsaBI BssHII Bst1107I BstZ17I CpoI EcoNI EcoRI FbaI FseI HpaI MamI MfeI MluI MunI NdeI NotI NruI PacI PaeR7I PmeI PvuI RsrII SalI SapI SbfI ScaI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AclI 4282 AACGTT AA^CGTT AflII 4888 CTTAAG C^TTAAG AgeI 3818 ACCGGT A^CCGGT AhdI 1518 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1763 ATTAAT AT^TAAT AsnI 1763 ATTAAT AT^TAAT Asp700I 947 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1518 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 845 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2193 TGCGCA TGC^GCA BfrI 4888 CTTAAG C^TTAAG BsiWI 291 CGTACG C^GTACG BsmBI 3918 CGTCTC CGTCTC(1/5) Bsp1407I 4478 TGTACA T^GTACA BspMI 234 ACCTGC ACCTGC(4/8) BsrGI 4478 TGTACA T^GTACA BssSI 3501 CTCGTG CACGAG(-5/-1) BstBI 1182 TTCGAA TT^CGAA DraI 1342 TTTAAA TTT^AAA DrdI 5281 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 501 CGGCCG C^GGCCG Eam1105I 1518 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 501 CGGCCG C^GGCCG Eco52I 501 CGGCCG C^GGCCG FspI 2193 TGCGCA TGC^GCA HgaI 3448 GACGC GACGC(5/10) HincII 3370 GTCAAC GT[CT]^[GA]AC HindII 3370 GTCAAC GT[CT]^[GA]AC HindIII 3770 AAGCTT A^AGCTT NspV 1182 TTCGAA TT^CGAA PflFI 845 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3818 ACCGGT A^CCGGT PpuMI 5279 AGGACCT [GA]G^G[AT]CC[CT] PshAI 4366 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1763 ATTAAT AT^TAAT Psp1406I 4282 AACGTT AA^CGTT SacI 3089 GAGCTC GAGCT^C SexAI 846 ACCAGGT A^CC[AT]GGT SfiI 214 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1182 TTCGAA TT^CGAA SphI 4497 GCATGC GCATG^C SspBI 4478 TGTACA T^GTACA Tth111I 845 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 947 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 2425 GGTACC G^GTACC 4632 GGTACC AccI 350 GTCTAC GT^[AC][GT]AC 3693 GTCTAC AccIII 834 TCCGGA T^CCGGA 3356 TCCGGA AcyI 1019 GGCGCC G[GA]^CG[CT]C 2715 GGCGCC Alw44I 3269 GTGCAC G^TGCAC 3307 GTGCAC AocI 3723 CCTAAGG CC^T[ACGT]AGG 3796 CCTGAGG Aor51HI 309 AGCGCT AGC^GCT 3612 AGCGCT ApaI 222 GGGCCC GGGCC^C 2769 GGGCCC ApaLI 3269 GTGCAC G^TGCAC 3307 GTGCAC Asp718I 2425 GGTACC G^GTACC 4632 GGTACC BbeI 1019 GGCGCC GGCGC^C 2715 GGCGCC BbrPI 806 CACGTG CAC^GTG 4872 CACGTG BglII 381 AGATCT A^GATCT 1800 AGATCT BsaHI 1019 GGCGCC G[GA]^CG[CT]C 2715 GGCGCC BsaI 3506 GAGACC GGTCTC(1/5) 4701 GGTCTC BseAI 834 TCCGGA T^CCGGA 3356 TCCGGA BsiEI 459 CGGTCG CG[GA][CT]^CG 501 CGGCCG BsmI 2603 GCATTC GAATGC(1/-1) 5165 GCATTC BspDI 744 ATCGAT AT^CGAT 1049 ATCGAT BspEI 834 TCCGGA T^CCGGA 3356 TCCGGA BspHI 2314 TCATGA T^CATGA 5121 TCATGA BstEII 2358 GGTTACC G^GT[ACGT]ACC 4412 GGTCACC BstPI 2358 GGTTACC G^GT[ACGT]ACC 4412 GGTCACC Bsu36I 3723 CCTAAGG CC^T[ACGT]AGG 3796 CCTGAGG ClaI 744 ATCGAT AT^CGAT 1049 ATCGAT DraIII 2411 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 2979 CACTTTGTG Eco47III 309 AGCGCT AGC^GCT 3612 AGCGCT Eco81I 3723 CCTAAGG CC^T[ACGT]AGG 3796 CCTGAGG EcoO65I 2358 GGTTACC G^GT[ACGT]ACC 4412 GGTCACC EcoRV 1412 GATATC GAT^ATC 3293 GATATC EcoT22I 2601 ATGCAT ATGCA^T 4820 ATGCAT Hin1I 1019 GGCGCC G[GA]^CG[CT]C 2715 GGCGCC KasI 1019 GGCGCC G^GCGCC 2715 GGCGCC KpnI 2425 GGTACC GGTAC^C 4632 GGTACC KspI 157 CCGCGG CCGC^GG 365 CCGCGG MroI 834 TCCGGA T^CCGGA 3356 TCCGGA NarI 1019 GGCGCC GG^CGCC 2715 GGCGCC NcoI 696 CCATGG C^CATGG 1654 CCATGG NheI 2906 GCTAGC G^CTAGC 4803 GCTAGC NsiI 2601 ATGCAT ATGCA^T 4820 ATGCAT PmaCI 806 CACGTG CAC^GTG 4872 CACGTG PmlI 806 CACGTG CAC^GTG 4872 CACGTG RcaI 2314 TCATGA T^CATGA 5121 TCATGA SacII 157 CCGCGG CCGC^GG 365 CCGCGG SfoI 1019 GGCGCC GGC^GCC 2715 GGCGCC StuI 2098 AGGCCT AGG^CCT 3325 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI