##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BamHI BbeI BcgI BciVI
BfrI BsiEI BsiWI BspDI BsrBI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI HpaI
KasI KspI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2963 GACGTC GACGT^C
Acc65I 3487 GGTACC G^GTACC
AccII 203 CGCG CG^CG
AcyI 2963 GACGTC G[GA]^CG[CT]C
Alw44I 5566 GTGCAC G^TGCAC
ApaLI 5566 GTGCAC G^TGCAC
Asp700I 1590 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3487 GGTACC G^GTACC
AspI 3770 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 900 CCTAGG C^CTAGG
BbrPI 1442 CACGTG CAC^GTG
BclI 660 TGATCA T^GATCA
BlnI 900 CCTAGG C^CTAGG
BsaAI 1442 CACGTG [CT]AC^GT[GA]
BsaBI 3523 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2963 GACGTC G[GA]^CG[CT]C
Bsp1407I 1583 TGTACA T^GTACA
BspHI 3066 TCATGA T^CATGA
BspLU11I 5562 ACATGT A^CATGT
BsrFI 4607 ACCGGC [GA]^CCGG[CT]
BsrGI 1583 TGTACA T^GTACA
BssSI 609 CTCGTG CACGAG(-5/-1)
BstUI 203 CGCG CG^CG
Cfr10I 4607 ACCGGC [GA]^CCGG[CT]
DrdI 2130 GACGTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2095 GAATTC G^AATTC
FbaI 660 TGATCA T^GATCA
Hin1I 2963 GACGTC G[GA]^CG[CT]C
KpnI 3487 GGTACC GGTAC^C
MamI 3523 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 203 CGCG CG^CG
NruI 202 TCGCGA TCG^CGA
PflFI 3770 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1442 CACGTG CAC^GTG
PmlI 1442 CACGTG CAC^GTG
PshAI 2959 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3066 TCATGA T^CATGA
SacI 1465 GAGCTC GAGCT^C
SbfI 2076 CCTGCAGG CCTGCA^GG
SmaI 26 CCCGGG CCC^GGG
SpeI 4852 ACTAGT A^CTAGT
Sse8387I 2076 CCTGCAGG CCTGCA^GG
SspBI 1583 TGTACA T^GTACA
SspI 1607 AATATT AAT^ATT
Tth111I 3770 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 26 CCCGGG C^CCGGG
XmaI 26 CCCGGG C^CCGGG
XmnI 1590 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 1443 ACGTGT A^C[GA][CT]GT
5562 ACATGT
AocI 1208 CCTGAGG CC^T[ACGT]AGG
2975 CCTCAGG
BlpI 3147 GCTTAGC GC^T[ACGT]AGC
5255 GCTCAGC
Bpu1102I 3147 GCTTAGC GC^T[ACGT]AGC
5255 GCTCAGC
BsmBI 2105 GAGACG CGTCTC(1/5)
2128 GAGACG
BsmI 5141 GCATTC GAATGC(1/-1)
5460 GCATTC
Bsu36I 1208 CCTGAGG CC^T[ACGT]AGG
2975 CCTCAGG
CelII 3147 GCTTAGC GC^T[ACGT]AGC
5255 GCTCAGC
Eco81I 1208 CCTGAGG CC^T[ACGT]AGG
2975 CCTCAGG
EcoT22I 442 ATGCAT ATGCA^T
5553 ATGCAT
HgaI 484 GCGTC GACGC(5/10)
3774 GCGTC
HindIII 1420 AAGCTT A^AGCTT
2432 AAGCTT
NheI 1860 GCTAGC G^CTAGC
5638 GCTAGC
NsiI 442 ATGCAT ATGCA^T
5553 ATGCAT
PaeR7I 141 CTCGAG C^TCGAG
2622 CTCGAG
XcmI 2645 CCACTCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3649 CCACAGGCTGCCTGG
XhoI 141 CTCGAG C^TCGAG
2622 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII