################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccI AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AviII BamHI BbeI BcgI BciVI BfrI BsiEI BsiWI BspDI BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HpaI KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 2963 GACGTC GACGT^C Acc65I 3487 GGTACC G^GTACC AccII 203 CGCG CG^CG AcyI 2963 GACGTC G[GA]^CG[CT]C Alw44I 5566 GTGCAC G^TGCAC ApaLI 5566 GTGCAC G^TGCAC Asp700I 1590 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3487 GGTACC G^GTACC AspI 3770 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 900 CCTAGG C^CTAGG BbrPI 1442 CACGTG CAC^GTG BclI 660 TGATCA T^GATCA BlnI 900 CCTAGG C^CTAGG BsaAI 1442 CACGTG [CT]AC^GT[GA] BsaBI 3523 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2963 GACGTC G[GA]^CG[CT]C Bsp1407I 1583 TGTACA T^GTACA BspHI 3066 TCATGA T^CATGA BspLU11I 5562 ACATGT A^CATGT BsrFI 4607 ACCGGC [GA]^CCGG[CT] BsrGI 1583 TGTACA T^GTACA BssSI 609 CTCGTG CACGAG(-5/-1) BstUI 203 CGCG CG^CG Cfr10I 4607 ACCGGC [GA]^CCGG[CT] DrdI 2130 GACGTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2095 GAATTC G^AATTC FbaI 660 TGATCA T^GATCA Hin1I 2963 GACGTC G[GA]^CG[CT]C KpnI 3487 GGTACC GGTAC^C MamI 3523 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 203 CGCG CG^CG NruI 202 TCGCGA TCG^CGA PflFI 3770 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1442 CACGTG CAC^GTG PmlI 1442 CACGTG CAC^GTG PshAI 2959 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3066 TCATGA T^CATGA SacI 1465 GAGCTC GAGCT^C SbfI 2076 CCTGCAGG CCTGCA^GG SmaI 26 CCCGGG CCC^GGG SpeI 4852 ACTAGT A^CTAGT Sse8387I 2076 CCTGCAGG CCTGCA^GG SspBI 1583 TGTACA T^GTACA SspI 1607 AATATT AAT^ATT Tth111I 3770 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 26 CCCGGG C^CCGGG XmaI 26 CCCGGG C^CCGGG XmnI 1590 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 1443 ACGTGT A^C[GA][CT]GT 5562 ACATGT AocI 1208 CCTGAGG CC^T[ACGT]AGG 2975 CCTCAGG BlpI 3147 GCTTAGC GC^T[ACGT]AGC 5255 GCTCAGC Bpu1102I 3147 GCTTAGC GC^T[ACGT]AGC 5255 GCTCAGC BsmBI 2105 GAGACG CGTCTC(1/5) 2128 GAGACG BsmI 5141 GCATTC GAATGC(1/-1) 5460 GCATTC Bsu36I 1208 CCTGAGG CC^T[ACGT]AGG 2975 CCTCAGG CelII 3147 GCTTAGC GC^T[ACGT]AGC 5255 GCTCAGC Eco81I 1208 CCTGAGG CC^T[ACGT]AGG 2975 CCTCAGG EcoT22I 442 ATGCAT ATGCA^T 5553 ATGCAT HgaI 484 GCGTC GACGC(5/10) 3774 GCGTC HindIII 1420 AAGCTT A^AGCTT 2432 AAGCTT NheI 1860 GCTAGC G^CTAGC 5638 GCTAGC NsiI 442 ATGCAT ATGCA^T 5553 ATGCAT PaeR7I 141 CTCGAG C^TCGAG 2622 CTCGAG XcmI 2645 CCACTCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3649 CCACAGGCTGCCTGG XhoI 141 CTCGAG C^TCGAG 2622 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccIII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII