##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BbrPI BlnI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
HpaI KspI MroI NdeI NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 1834 AACGTT AA^CGTT
AflII 72 CTTAAG C^TTAAG
AgeI 1183 ACCGGT A^CCGGT
AocI 4393 CCTCAGG CC^T[ACGT]AGG
BbvCI 3325 GCTGAGG CCTCAGC(-5/-2)
BclI 2723 TGATCA T^GATCA
BfrI 72 CTTAAG C^TTAAG
BglI 4935 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3034 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2475 GGTCTC GGTCTC(1/5)
BsmI 228 GAATGC GAATGC(1/-1)
BsrDI 1210 GCAATG GCAATG(2/0)
BstEII 878 GGTGACC G^GT[ACGT]ACC
BstPI 878 GGTGACC G^GT[ACGT]ACC
Bsu36I 4393 CCTCAGG CC^T[ACGT]AGG
Eco81I 4393 CCTCAGG CC^T[ACGT]AGG
EcoO65I 878 GGTGACC G^GT[ACGT]ACC
EcoRI 2587 GAATTC G^AATTC
EcoT22I 969 ATGCAT ATGCA^T
FbaI 2723 TGATCA T^GATCA
HindIII 1806 AAGCTT A^AGCTT
MamI 3034 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1411 CAATTG C^AATTG
MluI 2404 ACGCGT A^CGCGT
MunI 1411 CAATTG C^AATTG
NaeI 415 GCCGGC GCC^GGC
NgoMIV 415 GCCGGC G^CCGGC
NheI 717 GCTAGC G^CTAGC
NsiI 969 ATGCAT ATGCA^T
NspI 3804 GCATGC [GA]CATG^[CT]
PaeR7I 2545 CTCGAG C^TCGAG
PinAI 1183 ACCGGT A^CCGGT
Psp1406I 1834 AACGTT AA^CGTT
PvuII 4981 CAGCTG CAG^CTG
SacI 2956 GAGCTC GAGCT^C
SexAI 3111 ACCAGGT A^CC[AT]GGT
SfiI 4934 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3804 GCATGC GCATG^C
StuI 3175 AGGCCT AGG^CCT
XhoI 2545 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 2451 GGTACC G^GTACC
4412 GGTACC
AcsI 947 GAATTT [GA]^AATT[CT]
2587 GAATTC
ApoI 947 GAATTT [GA]^AATT[CT]
2587 GAATTC
Asp700I 2583 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3309 GAAGGGTTTC
Asp718I 2451 GGTACC G^GTACC
4412 GGTACC
AviII 2939 TGCGCA TGC^GCA
3002 TGCGCA
BamHI 67 GGATCC G^GATCC
1249 GGATCC
BbeI 3795 GGCGCC GGCGC^C
4572 GGCGCC
BglII 3719 AGATCT A^GATCT
4094 AGATCT
BsaAI 799 TACGTG [CT]AC^GT[GA]
1966 TACGTG
BsaWI 345 ACCGGA [AT]^CCGG[AT]
1183 ACCGGT
BsiEI 1949 CGGTCG CG[GA][CT]^CG
2676 CGACCG
BsmBI 1260 GAGACG CGTCTC(1/5)
2263 GAGACG
BssSI 4225 CTCGTG CACGAG(-5/-1)
4851 CTCGTG
BstAPI 3630 GCACTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3804 GCATGCGGTGC
EcoNI 464 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3534 CCTGTCTAAGG
FspI 2939 TGCGCA TGC^GCA
3002 TGCGCA
KasI 3795 GGCGCC G^GCGCC
4572 GGCGCC
KpnI 2451 GGTACC GGTAC^C
4412 GGTACC
NarI 3795 GGCGCC GG^CGCC
4572 GGCGCC
SfoI 3795 GGCGCC GGC^GCC
4572 GGCGCC
XcmI 679 CCATGAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3916 CCACTTCGGTTCTGG
XmnI 2583 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3309 GAAGGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI