##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AflIII AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII BamHI BcgI BglII BsiWI
BsmBI BspDI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV FseI
FspI KspI MfeI MluI MunI
NcoI NotI NruI NspV PacI
PmeI PshBI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3470 GACGTC GACGT^C
Acc65I 3109 GGTACC G^GTACC
AccIII 4789 TCCGGA T^CCGGA
AclI 4151 AACGTT AA^CGTT
AflII 4005 CTTAAG C^TTAAG
AgeI 1379 ACCGGT A^CCGGT
ApaI 2013 GGGCCC GGGCC^C
Asp700I 1407 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3109 GGTACC G^GTACC
AvrII 2474 CCTAGG C^CTAGG
BbeI 2437 GGCGCC GGCGC^C
BbrPI 2005 CACGTG CAC^GTG
BfrI 4005 CTTAAG C^TTAAG
BlnI 2474 CCTAGG C^CTAGG
BlpI 1073 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1073 GCTCAGC GC^T[ACGT]AGC
BseAI 4789 TCCGGA T^CCGGA
BspEI 4789 TCCGGA T^CCGGA
BspHI 793 TCATGA T^CATGA
BssSI 179 CTCGTG CACGAG(-5/-1)
BstAPI 1321 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1073 GCTCAGC GC^T[ACGT]AGC
EagI 3297 CGGCCG C^GGCCG
EclXI 3297 CGGCCG C^GGCCG
Eco52I 3297 CGGCCG C^GGCCG
EcoT22I 5050 ATGCAT ATGCA^T
HpaI 89 GTTAAC GTT^AAC
KasI 2437 GGCGCC G^GCGCC
KpnI 3109 GGTACC GGTAC^C
MroI 4789 TCCGGA T^CCGGA
NaeI 3295 GCCGGC GCC^GGC
NarI 2437 GGCGCC GG^CGCC
NgoMIV 3295 GCCGGC G^CCGGC
NsiI 5050 ATGCAT ATGCA^T
PaeR7I 471 CTCGAG C^TCGAG
PflMI 3476 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1379 ACCGGT A^CCGGT
PmaCI 2005 CACGTG CAC^GTG
PmlI 2005 CACGTG CAC^GTG
PshAI 2652 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4151 AACGTT AA^CGTT
RcaI 793 TCATGA T^CATGA
SacI 2291 GAGCTC GAGCT^C
ScaI 4819 AGTACT AGT^ACT
SfoI 2437 GGCGCC GGC^GCC
SgrAI 1378 CACCGGTG C[GA]^CCGG[CT]G
Van91I 3476 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1873 CCAACCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 471 CTCGAG C^TCGAG
XmnI 1407 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 213 CGCG CG^CG
3350 CGCG
AspI 1910 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4090 GACCCTGTC
BanII 2013 GGGCCC G[GA]GC[CT]^C
2291 GAGCTC
BciVI 961 GGATAC GTATCC(6/5)
5295 GTATCC
BsaAI 2005 CACGTG [CT]AC^GT[GA]
3141 TACGTG
BsiEI 2657 CGGTCG CG[GA][CT]^CG
3297 CGGCCG
BsrBI 2493 GAGCGG CCGCTC(-3/-3)
3379 GAGCGG
BstUI 213 CGCG CG^CG
3350 CGCG
BstYI 437 GGATCT [GA]^GATC[CT]
2138 GGATCT
HaeII 2437 GGCGCC [GA]GCGC^[CT]
4702 AGCGCC
MflI 437 GGATCT [GA]^GATC[CT]
2138 GGATCT
MvnI 213 CGCG CG^CG
3350 CGCG
NdeI 2805 CATATG CA^TATG
4681 CATATG
NheI 3824 GCTAGC G^CTAGC
4726 GCTAGC
NspI 54 GCATGT [GA]CATG^[CT]
3585 GCATGT
PflFI 1910 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4090 GACCCTGTC
StuI 467 AGGCCT AGG^CCT
2561 AGGCCT
Tth111I 1910 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4090 GACCCTGTC
XhoII 437 GGATCT [GA]^GATC[CT]
2138 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI