##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AgeI Aor51HI AscI BbeI BbrPI
BciVI BclI BsaBI BsiWI BspDI
BspLU11I BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
EagI EclXI Eco47III Eco52I EcoO65I
FbaI FseI HaeII KasI KspI
MamI MluI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PvuI SacII SalI SbfI ScaI
SfoI SfuI SgrAI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 5638 GACGTC GACGT^C
Acc65I 3297 GGTACC G^GTACC
AclI 6341 AACGTT AA^CGTT
AflII 1172 CTTAAG C^TTAAG
AflIII 3637 ACACGT A^C[GA][CT]GT
AhdI 218 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3977 GTGCAC G^TGCAC
ApaI 811 GGGCCC GGGCC^C
ApaLI 3977 GTGCAC G^TGCAC
AseI 5703 ATTAAT AT^TAAT
AsnI 5703 ATTAAT AT^TAAT
Asp700I 5909 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3297 GGTACC G^GTACC
AspEI 218 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1571 CCTAGG C^CTAGG
BamHI 3209 GGATCC G^GATCC
BcgI 407 GCAGTTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1172 CTTAAG C^TTAAG
BglII 4376 AGATCT A^GATCT
BlnI 1571 CCTAGG C^CTAGG
BlpI 5012 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5012 GCTCAGC GC^T[ACGT]AGC
BsaAI 4450 TACGTA [CT]AC^GT[GA]
BsiEI 4881 CGGTCG CG[GA][CT]^CG
BssSI 243 CACGAG CACGAG(-5/-1)
CelII 5012 GCTCAGC GC^T[ACGT]AGC
CpoI 1908 CGGTCCG CG^G[AT]CCG
Eam1105I 218 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4769 GAATTC G^AATTC
EcoRV 4141 GATATC GAT^ATC
EcoT22I 1830 ATGCAT ATGCA^T
HindIII 1169 AAGCTT A^AGCTT
KpnI 3297 GGTACC GGTAC^C
MfeI 5838 CAATTG C^AATTG
MunI 5838 CAATTG C^AATTG
NcoI 7 CCATGG C^CATGG
NsiI 1830 ATGCAT ATGCA^T
PaeR7I 2209 CTCGAG C^TCGAG
PshBI 5703 ATTAAT AT^TAAT
Psp1406I 6341 AACGTT AA^CGTT
RsrII 1908 CGGTCCG CG^G[AT]CCG
SfiI 2011 GGCCAGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 4450 TACGTA TAC^GTA
SpeI 6012 ACTAGT A^CTAGT
XhoI 2209 CTCGAG C^TCGAG
XmnI 5909 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 1204 TCCGGA T^CCGGA
5136 TCCGGA
AocI 2261 CCTCAGG CC^T[ACGT]AGG
5299 CCTCAGG
AspI 158 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2743 GACAAAGTC
AviII 596 TGCGCA TGC^GCA
5366 TGCGCA
BseAI 1204 TCCGGA T^CCGGA
5136 TCCGGA
BsmI 1818 GAATGC GAATGC(1/-1)
1828 GAATGC
BspEI 1204 TCCGGA T^CCGGA
5136 TCCGGA
BspMI 732 ACCTGC ACCTGC(4/8)
5514 GCAGGT
Bsu36I 2261 CCTCAGG CC^T[ACGT]AGG
5299 CCTCAGG
DraIII 3494 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
5312 CACCCAGTG
Eco81I 2261 CCTCAGG CC^T[ACGT]AGG
5299 CCTCAGG
FspI 596 TGCGCA TGC^GCA
5366 TGCGCA
MroI 1204 TCCGGA T^CCGGA
5136 TCCGGA
NheI 5420 GCTAGC G^CTAGC
5949 GCTAGC
PflFI 158 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2743 GACAAAGTC
PflMI 3213 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3752 CCAGGAGCTGG
PvuII 2045 CAGCTG CAG^CTG
5119 CAGCTG
SmaI 499 CCCGGG CCC^GGG
590 CCCGGG
Tth111I 158 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2743 GACAAAGTC
Van91I 3213 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3752 CCAGGAGCTGG
XmaCI 499 CCCGGG C^CCGGG
590 CCCGGG
XmaI 499 CCCGGG C^CCGGG
590 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII