################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AflII AgeI AhdI AscI Asp718I AspEI AvrII BamHI BbvCI BcgI BfrI BglI BglII BlnI BsaBI BsiWI BsmBI BspLU11I BsrDI BssHII BstBI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI HpaI KpnI KspI MamI MfeI MluI MunI NheI NotI NruI NsiI NspI NspV PacI PinAI PmeI PvuI RsrII SacII SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 4698 GACGTC GACGT^C AccIII 951 TCCGGA T^CCGGA AflIII 1315 ACGTGT A^C[GA][CT]GT Aor51HI 2992 AGCGCT AGC^GCT ApaI 4230 GGGCCC GGGCC^C AseI 2604 ATTAAT AT^TAAT AsnI 2604 ATTAAT AT^TAAT BbrPI 1777 CACGTG CAC^GTG BseAI 951 TCCGGA T^CCGGA BsiEI 5012 CGACCG CG[GA][CT]^CG BspDI 1293 ATCGAT AT^CGAT BspEI 951 TCCGGA T^CCGGA BsrBI 1167 GAGCGG CCGCTC(-3/-3) BsrFI 4892 GCCGGC [GA]^CCGG[CT] Bst1107I 5176 GTATAC GTA^TAC BstEII 2868 GGTCACC G^GT[ACGT]ACC BstPI 2868 GGTCACC G^GT[ACGT]ACC BstZ17I 5176 GTATAC GTA^TAC Cfr10I 4892 GCCGGC [GA]^CCGG[CT] ClaI 1293 ATCGAT AT^CGAT DrdI 4323 GACGACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2992 AGCGCT AGC^GCT EcoO65I 2868 GGTCACC G^GT[ACGT]ACC EcoRI 5039 GAATTC G^AATTC MroI 951 TCCGGA T^CCGGA NaeI 4892 GCCGGC GCC^GGC NdeI 3211 CATATG CA^TATG NgoMIV 4892 GCCGGC G^CCGGC PaeR7I 493 CTCGAG C^TCGAG PmaCI 1777 CACGTG CAC^GTG PmlI 1777 CACGTG CAC^GTG PpuMI 196 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 2604 ATTAAT AT^TAAT PvuII 3858 CAGCTG CAG^CTG SalI 5396 GTCGAC G^TCGAC XbaI 1130 TCTAGA T^CTAGA XhoI 493 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AclI 3049 AACGTT AA^CGTT 4826 AACGTT AspI 192 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4326 GACACTGTC BciVI 2682 GTATCC GTATCC(6/5) 5641 GTATCC Bsp1407I 1879 TGTACA T^GTACA 3122 TGTACA BspMI 2865 GCAGGT ACCTGC(4/8) 4947 GCAGGT BsrGI 1879 TGTACA T^GTACA 3122 TGTACA BssSI 1783 CACGAG CACGAG(-5/-1) 2785 CTCGTG BstYI 545 GGATCT [GA]^GATC[CT] 877 GGATCT DraII 196 AGGTCCT [GA]G^G[ACGT]CC[CT] 4230 GGGCCCT EcoO109I 196 AGGTCCT [GA]G^G[ACGT]CC[CT] 4230 GGGCCCT HindIII 4131 AAGCTT A^AGCTT 5609 AAGCTT MflI 545 GGATCT [GA]^GATC[CT] 877 GGATCT MspA1I 500 CCGCTG C[AC]G^C[GT]G 3858 CAGCTG PflFI 192 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4326 GACACTGTC PflMI 573 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3789 CCAAAATGTGG PshAI 99 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2012 GACCTTTGTC Psp1406I 3049 AACGTT AA^CGTT 4826 AACGTT ScaI 4436 AGTACT AGT^ACT 4975 AGTACT SspBI 1879 TGTACA T^GTACA 3122 TGTACA Tth111I 192 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4326 GACACTGTC Van91I 573 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3789 CCAAAATGTGG XcmI 203 CCAACAGCTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1703 CCAGAAAATCCATGG XhoII 545 GGATCT [GA]^GATC[CT] 877 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EaeI EarI Eco81I EcoNI EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PleI PstI RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI