##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AflII AgeI AhdI AscI
Asp718I AspEI AvrII BamHI BbvCI
BcgI BfrI BglI BglII BlnI
BsaBI BsiWI BsmBI BspLU11I BsrDI
BssHII BstBI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI HpaI KpnI KspI
MamI MfeI MluI MunI NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PvuI RsrII
SacII SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 4698 GACGTC GACGT^C
AccIII 951 TCCGGA T^CCGGA
AflIII 1315 ACGTGT A^C[GA][CT]GT
Aor51HI 2992 AGCGCT AGC^GCT
ApaI 4230 GGGCCC GGGCC^C
AseI 2604 ATTAAT AT^TAAT
AsnI 2604 ATTAAT AT^TAAT
BbrPI 1777 CACGTG CAC^GTG
BseAI 951 TCCGGA T^CCGGA
BsiEI 5012 CGACCG CG[GA][CT]^CG
BspDI 1293 ATCGAT AT^CGAT
BspEI 951 TCCGGA T^CCGGA
BsrBI 1167 GAGCGG CCGCTC(-3/-3)
BsrFI 4892 GCCGGC [GA]^CCGG[CT]
Bst1107I 5176 GTATAC GTA^TAC
BstEII 2868 GGTCACC G^GT[ACGT]ACC
BstPI 2868 GGTCACC G^GT[ACGT]ACC
BstZ17I 5176 GTATAC GTA^TAC
Cfr10I 4892 GCCGGC [GA]^CCGG[CT]
ClaI 1293 ATCGAT AT^CGAT
DrdI 4323 GACGACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2992 AGCGCT AGC^GCT
EcoO65I 2868 GGTCACC G^GT[ACGT]ACC
EcoRI 5039 GAATTC G^AATTC
MroI 951 TCCGGA T^CCGGA
NaeI 4892 GCCGGC GCC^GGC
NdeI 3211 CATATG CA^TATG
NgoMIV 4892 GCCGGC G^CCGGC
PaeR7I 493 CTCGAG C^TCGAG
PmaCI 1777 CACGTG CAC^GTG
PmlI 1777 CACGTG CAC^GTG
PpuMI 196 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2604 ATTAAT AT^TAAT
PvuII 3858 CAGCTG CAG^CTG
SalI 5396 GTCGAC G^TCGAC
XbaI 1130 TCTAGA T^CTAGA
XhoI 493 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AclI 3049 AACGTT AA^CGTT
4826 AACGTT
AspI 192 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4326 GACACTGTC
BciVI 2682 GTATCC GTATCC(6/5)
5641 GTATCC
Bsp1407I 1879 TGTACA T^GTACA
3122 TGTACA
BspMI 2865 GCAGGT ACCTGC(4/8)
4947 GCAGGT
BsrGI 1879 TGTACA T^GTACA
3122 TGTACA
BssSI 1783 CACGAG CACGAG(-5/-1)
2785 CTCGTG
BstYI 545 GGATCT [GA]^GATC[CT]
877 GGATCT
DraII 196 AGGTCCT [GA]G^G[ACGT]CC[CT]
4230 GGGCCCT
EcoO109I 196 AGGTCCT [GA]G^G[ACGT]CC[CT]
4230 GGGCCCT
HindIII 4131 AAGCTT A^AGCTT
5609 AAGCTT
MflI 545 GGATCT [GA]^GATC[CT]
877 GGATCT
MspA1I 500 CCGCTG C[AC]G^C[GT]G
3858 CAGCTG
PflFI 192 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4326 GACACTGTC
PflMI 573 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3789 CCAAAATGTGG
PshAI 99 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2012 GACCTTTGTC
Psp1406I 3049 AACGTT AA^CGTT
4826 AACGTT
ScaI 4436 AGTACT AGT^ACT
4975 AGTACT
SspBI 1879 TGTACA T^GTACA
3122 TGTACA
Tth111I 192 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4326 GACACTGTC
Van91I 573 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3789 CCAAAATGTGG
XcmI 203 CCAACAGCTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1703 CCAGAAAATCCATGG
XhoII 545 GGATCT [GA]^GATC[CT]
877 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI Asp700I AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
Eco81I EcoNI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI