##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AvrII
BamHI BlnI BlpI Bpu1102I BsiEI
BsiWI BspHI BsrBI BssHII Bst1107I
BstZ17I CelII CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI HpaI
KspI MluI NotI NruI PacI
PaeR7I PinAI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SpeI Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 5300 GGTACC G^GTACC
AcyI 538 GGCGCC G[GA]^CG[CT]C
AocI 1192 CCTGAGG CC^T[ACGT]AGG
Asp718I 5300 GGTACC G^GTACC
AspI 490 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 606 TGCGCA TGC^GCA
BalI 2442 TGGCCA TGG^CCA
BbeI 538 GGCGCC GGCGC^C
BbrPI 3751 CACGTG CAC^GTG
BbsI 4031 GAAGAC GAAGAC(2/6)
BbvCI 22 CCTCAGC CCTCAGC(-5/-2)
BclI 4710 TGATCA T^GATCA
BpuAI 4031 GAAGAC GAAGAC(2/6)
BsaBI 3272 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 538 GGCGCC G[GA]^CG[CT]C
BssSI 670 CACGAG CACGAG(-5/-1)
BstEII 1256 GGTCACC G^GT[ACGT]ACC
BstPI 1256 GGTCACC G^GT[ACGT]ACC
Bsu36I 1192 CCTGAGG CC^T[ACGT]AGG
DraI 796 TTTAAA TTT^AAA
DrdI 3314 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2442 TGGCCA [CT]^GGCC[GA]
Eco81I 1192 CCTGAGG CC^T[ACGT]AGG
EcoNI 4333 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1256 GGTCACC G^GT[ACGT]ACC
EcoT22I 1754 ATGCAT ATGCA^T
FbaI 4710 TGATCA T^GATCA
FspI 606 TGCGCA TGC^GCA
Hin1I 538 GGCGCC G[GA]^CG[CT]C
HincII 920 GTTGAC GT[CT]^[GA]AC
HindII 920 GTTGAC GT[CT]^[GA]AC
KasI 538 GGCGCC G^GCGCC
KpnI 5300 GGTACC GGTAC^C
MamI 3272 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1827 CAATTG C^AATTG
MluNI 2442 TGGCCA TGG^CCA
MscI 2442 TGGCCA TGG^CCA
MunI 1827 CAATTG C^AATTG
NaeI 3116 GCCGGC GCC^GGC
NarI 538 GGCGCC GG^CGCC
NgoMIV 3116 GCCGGC G^CCGGC
NheI 1801 GCTAGC G^CTAGC
NsiI 1754 ATGCAT ATGCA^T
PflFI 490 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3751 CACGTG CAC^GTG
PmeI 795 GTTTAAAC GTTT^AAAC
PmlI 3751 CACGTG CAC^GTG
SapI 274 GAAGAGC GCTCTTC(1/4)
SfoI 538 GGCGCC GGC^GCC
SmaI 353 CCCGGG CCC^GGG
SnaBI 2136 TACGTA TAC^GTA
SphI 4305 GCATGC GCATG^C
Tth111I 490 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 353 CCCGGG C^CCGGG
XmaI 353 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 4380 TCCGGA T^CCGGA
4416 TCCGGA
AflII 773 CTTAAG C^TTAAG
2806 CTTAAG
Alw44I 3406 GTGCAC G^TGCAC
3576 GTGCAC
ApaI 1180 GGGCCC GGGCC^C
4547 GGGCCC
ApaLI 3406 GTGCAC G^TGCAC
3576 GTGCAC
AvaI 353 CCCGGG C^[CT]CG[GA]G
839 CTCGGG
BcgI 2176 CGAGGAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3281 CGATGTACCTGC
BfrI 773 CTTAAG C^TTAAG
2806 CTTAAG
BglI 4506 GCCAATCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4647 GCCTGGAGGGC
BsaAI 2136 TACGTA [CT]AC^GT[GA]
3751 CACGTG
BseAI 4380 TCCGGA T^CCGGA
4416 TCCGGA
BsmBI 2072 CGTCTC CGTCTC(1/5)
5051 CGTCTC
BsoBI 353 CCCGGG C^[CT]CG[GA]G
839 CTCGGG
Bsp1407I 1164 TGTACA T^GTACA
5083 TGTACA
BspDI 1932 ATCGAT AT^CGAT
3279 ATCGAT
BspEI 4380 TCCGGA T^CCGGA
4416 TCCGGA
BspLU11I 3173 ACATGT A^CATGT
3518 ACATGT
BsrGI 1164 TGTACA T^GTACA
5083 TGTACA
BstAPI 1499 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3107 GCACGCTGTGC
BstBI 1583 TTCGAA TT^CGAA
4967 TTCGAA
BstXI 4339 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4924 CCAGTGCTGTGG
ClaI 1932 ATCGAT AT^CGAT
3279 ATCGAT
EarI 2934 CTCTTC CTCTTC(1/4)
274 GAAGAG
EcoRV 2171 GATATC GAT^ATC
3191 GATATC
HaeII 378 AGCGCC [GA]GCGC^[CT]
538 GGCGCC
Ksp632I 2934 CTCTTC CTCTTC(1/4)
274 GAAGAG
MroI 4380 TCCGGA T^CCGGA
4416 TCCGGA
NspV 1583 TTCGAA TT^CGAA
4967 TTCGAA
PflMI 382 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4177 CCACATTGTGG
SacI 138 GAGCTC GAGCT^C
984 GAGCTC
SfuI 1583 TTCGAA TT^CGAA
4967 TTCGAA
SspBI 1164 TGTACA T^GTACA
5083 TGTACA
Van91I 382 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4177 CCACATTGTGG
XbaI 5055 TCTAGA T^CTAGA
5495 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanI BanII
BbvI BciVI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI