################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccII AclI AflIII AgeI ApaI AscI Asp700I Asp718I BbrPI BglI BsaAI BsaBI BsiEI BsiWI BspDI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRV EcoT22I FseI HpaI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SspI StuI SwaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2375 GTAGAC GT^[AC][GT]AC AccIII 576 TCCGGA T^CCGGA AflII 3557 CTTAAG C^TTAAG AhdI 370 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5079 GTGCAC G^TGCAC Aor51HI 627 AGCGCT AGC^GCT ApaLI 5079 GTGCAC G^TGCAC AseI 5348 ATTAAT AT^TAAT AsnI 5348 ATTAAT AT^TAAT AspEI 370 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5026 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1260 TGGCCA TGG^CCA BamHI 10 GGATCC G^GATCC BbeI 5126 GGCGCC GGCGC^C BcgI 2786 CGATGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 264 GTATCC GTATCC(6/5) BfrI 3557 CTTAAG C^TTAAG BsaWI 576 TCCGGA [AT]^CCGG[AT] BseAI 576 TCCGGA T^CCGGA BsmBI 2182 GAGACG CGTCTC(1/5) BspEI 576 TCCGGA T^CCGGA BspHI 5608 TCATGA T^CATGA BsrFI 1686 GCCGGT [GA]^CCGG[CT] Cfr10I 1686 GCCGGT [GA]^CCGG[CT] DraIII 5500 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 822 CCATGG C^C[GA][CT]GG Eam1105I 370 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 627 AGCGCT AGC^GCT EcoRI 875 GAATTC G^AATTC KasI 5126 GGCGCC G^GCGCC MaeII 814 ACGT A^CGT MluNI 1260 TGGCCA TGG^CCA MroI 576 TCCGGA T^CCGGA MscI 1260 TGGCCA TGG^CCA NarI 5126 GGCGCC GG^CGCC NcoI 822 CCATGG C^CATGG NdeI 336 CATATG CA^TATG PaeR7I 24 CTCGAG C^TCGAG PflFI 5026 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 5348 ATTAAT AT^TAAT RcaI 5608 TCATGA T^CATGA ScaI 469 AGTACT AGT^ACT SfoI 5126 GGCGCC GGC^GCC SpeI 2387 ACTAGT A^CTAGT Tth111I 5026 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 24 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 875 GAATTC [GA]^AATT[CT] 2307 AAATTT ApoI 875 GAATTC [GA]^AATT[CT] 2307 AAATTT AvrII 2922 CCTAGG C^CTAGG 4013 CCTAGG BbvCI 4993 CCTCAGC CCTCAGC(-5/-2) 2951 GCTGAGG BclI 340 TGATCA T^GATCA 2939 TGATCA BglII 2504 AGATCT A^GATCT 3610 AGATCT BlnI 2922 CCTAGG C^CTAGG 4013 CCTAGG BlpI 517 GCTGAGC GC^T[ACGT]AGC 994 GCTCAGC Bpu1102I 517 GCTGAGC GC^T[ACGT]AGC 994 GCTCAGC BsmI 2062 GAATGC GAATGC(1/-1) 2693 GAATGC Bsp1407I 2724 TGTACA T^GTACA 3701 TGTACA BsrGI 2724 TGTACA T^GTACA 3701 TGTACA CelII 517 GCTGAGC GC^T[ACGT]AGC 994 GCTCAGC DrdI 3952 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4011 GACCTAGGAGTC EaeI 1260 TGGCCA [CT]^GGCC[GA] 1429 TGGCCG FauI 224 CCCGC CCCGC(4/6) 4839 GCGGG FbaI 340 TGATCA T^GATCA 2939 TGATCA HaeII 627 AGCGCT [GA]GCGC^[CT] 5126 GGCGCC PpuMI 1568 AGGACCT [GA]G^G[AT]CC[CT] 2128 AGGTCCT SacI 572 GAGCTC GAGCT^C 4768 GAGCTC SapI 4296 GAAGAGC GCTCTTC(1/4) 5290 GAAGAGC SexAI 1579 ACCTGGT A^CC[AT]GGT 4031 ACCTGGT SspBI 2724 TGTACA T^GTACA 3701 TGTACA TaqI 25 TCGA T^CGA 3197 TCGA TthHB8I 25 TCGA T^CGA 3197 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII AviII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI FspI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoII XmaCI XmaI