##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccII AclI AflIII
AgeI ApaI AscI Asp700I Asp718I
BbrPI BglI BsaAI BsaBI BsiEI
BsiWI BspDI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRV EcoT22I FseI HpaI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SspI
StuI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2375 GTAGAC GT^[AC][GT]AC
AccIII 576 TCCGGA T^CCGGA
AflII 3557 CTTAAG C^TTAAG
AhdI 370 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5079 GTGCAC G^TGCAC
Aor51HI 627 AGCGCT AGC^GCT
ApaLI 5079 GTGCAC G^TGCAC
AseI 5348 ATTAAT AT^TAAT
AsnI 5348 ATTAAT AT^TAAT
AspEI 370 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5026 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1260 TGGCCA TGG^CCA
BamHI 10 GGATCC G^GATCC
BbeI 5126 GGCGCC GGCGC^C
BcgI 2786 CGATGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 264 GTATCC GTATCC(6/5)
BfrI 3557 CTTAAG C^TTAAG
BsaWI 576 TCCGGA [AT]^CCGG[AT]
BseAI 576 TCCGGA T^CCGGA
BsmBI 2182 GAGACG CGTCTC(1/5)
BspEI 576 TCCGGA T^CCGGA
BspHI 5608 TCATGA T^CATGA
BsrFI 1686 GCCGGT [GA]^CCGG[CT]
Cfr10I 1686 GCCGGT [GA]^CCGG[CT]
DraIII 5500 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 822 CCATGG C^C[GA][CT]GG
Eam1105I 370 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 627 AGCGCT AGC^GCT
EcoRI 875 GAATTC G^AATTC
KasI 5126 GGCGCC G^GCGCC
MaeII 814 ACGT A^CGT
MluNI 1260 TGGCCA TGG^CCA
MroI 576 TCCGGA T^CCGGA
MscI 1260 TGGCCA TGG^CCA
NarI 5126 GGCGCC GG^CGCC
NcoI 822 CCATGG C^CATGG
NdeI 336 CATATG CA^TATG
PaeR7I 24 CTCGAG C^TCGAG
PflFI 5026 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5348 ATTAAT AT^TAAT
RcaI 5608 TCATGA T^CATGA
ScaI 469 AGTACT AGT^ACT
SfoI 5126 GGCGCC GGC^GCC
SpeI 2387 ACTAGT A^CTAGT
Tth111I 5026 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 24 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 875 GAATTC [GA]^AATT[CT]
2307 AAATTT
ApoI 875 GAATTC [GA]^AATT[CT]
2307 AAATTT
AvrII 2922 CCTAGG C^CTAGG
4013 CCTAGG
BbvCI 4993 CCTCAGC CCTCAGC(-5/-2)
2951 GCTGAGG
BclI 340 TGATCA T^GATCA
2939 TGATCA
BglII 2504 AGATCT A^GATCT
3610 AGATCT
BlnI 2922 CCTAGG C^CTAGG
4013 CCTAGG
BlpI 517 GCTGAGC GC^T[ACGT]AGC
994 GCTCAGC
Bpu1102I 517 GCTGAGC GC^T[ACGT]AGC
994 GCTCAGC
BsmI 2062 GAATGC GAATGC(1/-1)
2693 GAATGC
Bsp1407I 2724 TGTACA T^GTACA
3701 TGTACA
BsrGI 2724 TGTACA T^GTACA
3701 TGTACA
CelII 517 GCTGAGC GC^T[ACGT]AGC
994 GCTCAGC
DrdI 3952 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4011 GACCTAGGAGTC
EaeI 1260 TGGCCA [CT]^GGCC[GA]
1429 TGGCCG
FauI 224 CCCGC CCCGC(4/6)
4839 GCGGG
FbaI 340 TGATCA T^GATCA
2939 TGATCA
HaeII 627 AGCGCT [GA]GCGC^[CT]
5126 GGCGCC
PpuMI 1568 AGGACCT [GA]G^G[AT]CC[CT]
2128 AGGTCCT
SacI 572 GAGCTC GAGCT^C
4768 GAGCTC
SapI 4296 GAAGAGC GCTCTTC(1/4)
5290 GAAGAGC
SexAI 1579 ACCTGGT A^CC[AT]GGT
4031 ACCTGGT
SspBI 2724 TGTACA T^GTACA
3701 TGTACA
TaqI 25 TCGA T^CGA
3197 TCGA
TthHB8I 25 TCGA T^CGA
3197 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
AviII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII XmaCI XmaI