##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI Asp700I Asp718I BcgI BciVI
BclI BfrI BsaI BsiEI BspDI
BssHII BssSI BstBI BstEII BstPI
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
HpaI KpnI KspI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NspV PaeR7I PinAI PmeI
PvuI RsrII SacI SacII SalI
SfiI SfuI SgrAI SmaI SnaBI
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 1851 AACGTT AA^CGTT
AcyI 3852 GGCGCC G[GA]^CG[CT]C
Alw44I 4671 GTGCAC G^TGCAC
ApaLI 4671 GTGCAC G^TGCAC
AseI 1498 ATTAAT AT^TAAT
AsnI 1498 ATTAAT AT^TAAT
AspI 1393 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4935 CTCGGG C^[CT]CG[GA]G
AvrII 3250 CCTAGG C^CTAGG
BamHI 584 GGATCC G^GATCC
BbeI 3852 GGCGCC GGCGC^C
BlnI 3250 CCTAGG C^CTAGG
BlpI 1981 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1981 GCTCAGC GC^T[ACGT]AGC
BsaBI 2807 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3852 GGCGCC G[GA]^CG[CT]C
BsiWI 370 CGTACG C^GTACG
BsoBI 4935 CTCGGG C^[CT]CG[GA]G
BsrBI 2012 CCGCTC CCGCTC(-3/-3)
BsrDI 253 GCAATG GCAATG(2/0)
Bst1107I 1699 GTATAC GTA^TAC
BstZ17I 1699 GTATAC GTA^TAC
CelII 1981 GCTCAGC GC^T[ACGT]AGC
DraIII 665 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 285 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 982 GAATTC G^AATTC
Hin1I 3852 GGCGCC G[GA]^CG[CT]C
HincII 777 GTCAAC GT[CT]^[GA]AC
HindII 777 GTCAAC GT[CT]^[GA]AC
KasI 3852 GGCGCC G^GCGCC
MamI 2807 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3852 GGCGCC GG^CGCC
PacI 1495 TTAATTAA TTAAT^TAA
PflFI 1393 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3089 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1498 ATTAAT AT^TAAT
Psp1406I 1851 AACGTT AA^CGTT
SapI 3001 GCTCTTC GCTCTTC(1/4)
SexAI 3088 ACCAGGT A^CC[AT]GGT
SfoI 3852 GGCGCC GGC^GCC
SphI 2331 GCATGC GCATG^C
Tth111I 1393 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3089 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1911 TCCGGA T^CCGGA
2935 TCCGGA
AocI 839 CCTAAGG CC^T[ACGT]AGG
1584 CCTAAGG
AviII 3792 TGCGCA TGC^GCA
3835 TGCGCA
BanI 2792 GGTGCC G^G[CT][GA]CC
3852 GGCGCC
BbvCI 1347 CCTCAGC CCTCAGC(-5/-2)
4482 GCTGAGG
BglI 1964 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4913 GCCAAAGGGGC
BglII 4042 AGATCT A^GATCT
4494 AGATCT
BsaWI 1911 TCCGGA [AT]^CCGG[AT]
2935 TCCGGA
BseAI 1911 TCCGGA T^CCGGA
2935 TCCGGA
BsmBI 3742 CGTCTC CGTCTC(1/5)
2787 GAGACG
BsmI 305 GAATGC GAATGC(1/-1)
1692 GCATTC
BspEI 1911 TCCGGA T^CCGGA
2935 TCCGGA
BspHI 607 TCATGA T^CATGA
1370 TCATGA
BspLU11I 794 ACATGT A^CATGT
2588 ACATGT
BsrFI 13 ACCGGC [GA]^CCGG[CT]
3290 ACCGGC
Bsu36I 839 CCTAAGG CC^T[ACGT]AGG
1584 CCTAAGG
Cfr10I 13 ACCGGC [GA]^CCGG[CT]
3290 ACCGGC
Eco81I 839 CCTAAGG CC^T[ACGT]AGG
1584 CCTAAGG
EcoNI 2223 CCTCCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3456 CCTTTTGAAGG
FauI 3505 GCGGG CCCGC(4/6)
3912 GCGGG
FspI 3792 TGCGCA TGC^GCA
3835 TGCGCA
HaeII 2235 GGCGCT [GA]GCGC^[CT]
3852 GGCGCC
HindIII 4162 AAGCTT A^AGCTT
4953 AAGCTT
MroI 1911 TCCGGA T^CCGGA
2935 TCCGGA
NdeI 1416 CATATG CA^TATG
3134 CATATG
PshAI 1735 GACACTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3961 GACAACTGTC
RcaI 607 TCATGA T^CATGA
1370 TCATGA
SbfI 3807 CCTGCAGG CCTGCA^GG
4469 CCTGCAGG
SpeI 1738 ACTAGT A^CTAGT
2083 ACTAGT
Sse8387I 3807 CCTGCAGG CCTGCA^GG
4469 CCTGCAGG
SspI 2115 AATATT AAT^ATT
4195 AATATT
StuI 361 AGGCCT AGG^CCT
640 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaII
BalI BanII BbrPI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII