################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AflII AgeI Aor51HI AscI Asp700I Asp718I BcgI BciVI BclI BfrI BsaI BsiEI BspDI BssHII BssSI BstBI BstEII BstPI ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI HpaI KpnI KspI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NspV PaeR7I PinAI PmeI PvuI RsrII SacI SacII SalI SfiI SfuI SgrAI SmaI SnaBI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 1851 AACGTT AA^CGTT AcyI 3852 GGCGCC G[GA]^CG[CT]C Alw44I 4671 GTGCAC G^TGCAC ApaLI 4671 GTGCAC G^TGCAC AseI 1498 ATTAAT AT^TAAT AsnI 1498 ATTAAT AT^TAAT AspI 1393 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 4935 CTCGGG C^[CT]CG[GA]G AvrII 3250 CCTAGG C^CTAGG BamHI 584 GGATCC G^GATCC BbeI 3852 GGCGCC GGCGC^C BlnI 3250 CCTAGG C^CTAGG BlpI 1981 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1981 GCTCAGC GC^T[ACGT]AGC BsaBI 2807 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3852 GGCGCC G[GA]^CG[CT]C BsiWI 370 CGTACG C^GTACG BsoBI 4935 CTCGGG C^[CT]CG[GA]G BsrBI 2012 CCGCTC CCGCTC(-3/-3) BsrDI 253 GCAATG GCAATG(2/0) Bst1107I 1699 GTATAC GTA^TAC BstZ17I 1699 GTATAC GTA^TAC CelII 1981 GCTCAGC GC^T[ACGT]AGC DraIII 665 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 285 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 982 GAATTC G^AATTC Hin1I 3852 GGCGCC G[GA]^CG[CT]C HincII 777 GTCAAC GT[CT]^[GA]AC HindII 777 GTCAAC GT[CT]^[GA]AC KasI 3852 GGCGCC G^GCGCC MamI 2807 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 3852 GGCGCC GG^CGCC PacI 1495 TTAATTAA TTAAT^TAA PflFI 1393 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3089 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1498 ATTAAT AT^TAAT Psp1406I 1851 AACGTT AA^CGTT SapI 3001 GCTCTTC GCTCTTC(1/4) SexAI 3088 ACCAGGT A^CC[AT]GGT SfoI 3852 GGCGCC GGC^GCC SphI 2331 GCATGC GCATG^C Tth111I 1393 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3089 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 1911 TCCGGA T^CCGGA 2935 TCCGGA AocI 839 CCTAAGG CC^T[ACGT]AGG 1584 CCTAAGG AviII 3792 TGCGCA TGC^GCA 3835 TGCGCA BanI 2792 GGTGCC G^G[CT][GA]CC 3852 GGCGCC BbvCI 1347 CCTCAGC CCTCAGC(-5/-2) 4482 GCTGAGG BglI 1964 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4913 GCCAAAGGGGC BglII 4042 AGATCT A^GATCT 4494 AGATCT BsaWI 1911 TCCGGA [AT]^CCGG[AT] 2935 TCCGGA BseAI 1911 TCCGGA T^CCGGA 2935 TCCGGA BsmBI 3742 CGTCTC CGTCTC(1/5) 2787 GAGACG BsmI 305 GAATGC GAATGC(1/-1) 1692 GCATTC BspEI 1911 TCCGGA T^CCGGA 2935 TCCGGA BspHI 607 TCATGA T^CATGA 1370 TCATGA BspLU11I 794 ACATGT A^CATGT 2588 ACATGT BsrFI 13 ACCGGC [GA]^CCGG[CT] 3290 ACCGGC Bsu36I 839 CCTAAGG CC^T[ACGT]AGG 1584 CCTAAGG Cfr10I 13 ACCGGC [GA]^CCGG[CT] 3290 ACCGGC Eco81I 839 CCTAAGG CC^T[ACGT]AGG 1584 CCTAAGG EcoNI 2223 CCTCCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3456 CCTTTTGAAGG FauI 3505 GCGGG CCCGC(4/6) 3912 GCGGG FspI 3792 TGCGCA TGC^GCA 3835 TGCGCA HaeII 2235 GGCGCT [GA]GCGC^[CT] 3852 GGCGCC HindIII 4162 AAGCTT A^AGCTT 4953 AAGCTT MroI 1911 TCCGGA T^CCGGA 2935 TCCGGA NdeI 1416 CATATG CA^TATG 3134 CATATG PshAI 1735 GACACTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3961 GACAACTGTC RcaI 607 TCATGA T^CATGA 1370 TCATGA SbfI 3807 CCTGCAGG CCTGCA^GG 4469 CCTGCAGG SpeI 1738 ACTAGT A^CTAGT 2083 ACTAGT Sse8387I 3807 CCTGCAGG CCTGCA^GG 4469 CCTGCAGG SspI 2115 AATATT AAT^ATT 4195 AATATT StuI 361 AGGCCT AGG^CCT 640 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaII BalI BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII