################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI BamHI BbeI BbrPI BsaHI BsiWI BspDI BspLU11I BstBI BstEII BstPI ClaI CpoI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI Hin1I HindIII KasI MfeI MunI NarI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 3937 TCCGGA T^CCGGA AspI 4928 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4393 TGCGCA TGC^GCA BcgI 452 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 5057 TGATCA T^GATCA BsaAI 509 CACGTA [CT]AC^GT[GA] BsaBI 1617 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1493 GGTCTC GGTCTC(1/5) BseAI 3937 TCCGGA T^CCGGA BsmBI 64 GAGACG CGTCTC(1/5) BsmI 2889 GAATGC GAATGC(1/-1) Bsp1407I 3812 TGTACA T^GTACA BspEI 3937 TCCGGA T^CCGGA BsrGI 3812 TGTACA T^GTACA BssSI 1723 CACGAG CACGAG(-5/-1) Bst1107I 4824 GTATAC GTA^TAC BstZ17I 4824 GTATAC GTA^TAC DraI 5028 TTTAAA TTT^AAA DraIII 1966 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 20 CGGCCG C^GGCCG EclXI 20 CGGCCG C^GGCCG Eco52I 20 CGGCCG C^GGCCG FbaI 5057 TGATCA T^GATCA FspI 4393 TGCGCA TGC^GCA HgaI 66 GACGC GACGC(5/10) HpaI 4761 GTTAAC GTT^AAC KspI 76 CCGCGG CCGC^GG MamI 1617 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 67 ACGCGT A^CGCGT MroI 3937 TCCGGA T^CCGGA NaeI 1675 GCCGGC GCC^GGC NgoMIV 1675 GCCGGC G^CCGGC PaeR7I 873 CTCGAG C^TCGAG PflFI 4928 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 371 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2334 GAGCTC GAGCT^C SacII 76 CCGCGG CCGC^GG ScaI 5082 AGTACT AGT^ACT SspBI 3812 TGTACA T^GTACA Tth111I 4928 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4533 TCTAGA T^CTAGA XhoI 873 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 3489 CTTAAG C^TTAAG 5399 CTTAAG AflIII 67 ACGCGT A^C[GA][CT]GT 508 ACACGT AocI 562 CCTCAGG CC^T[ACGT]AGG 2409 CCTGAGG AspHI 2334 GAGCTC G[AT]GC[AT]^C 3208 GTGCTC BfrI 3489 CTTAAG C^TTAAG 5399 CTTAAG BsiEI 20 CGGCCG CG[GA][CT]^CG 3587 CGGTCG BsiHKAI 2334 GAGCTC G[AT]GC[AT]^C 3208 GTGCTC BsrBI 2667 CCGCTC CCGCTC(-3/-3) 447 GAGCGG BsrFI 1675 GCCGGC [GA]^CCGG[CT] 3585 GCCGGT BssHII 81 GCGCGC G^CGCGC 83 GCGCGC Bsu36I 562 CCTCAGG CC^T[ACGT]AGG 2409 CCTGAGG Cfr10I 1675 GCCGGC [GA]^CCGG[CT] 3585 GCCGGT DrdI 1980 GACCGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5130 GACAGAATGGTC Eco81I 562 CCTCAGG CC^T[ACGT]AGG 2409 CCTGAGG HincII 620 GTTGAC GT[CT]^[GA]AC 4761 GTTAAC HindII 620 GTTGAC GT[CT]^[GA]AC 4761 GTTAAC NdeI 4406 CATATG CA^TATG 5253 CATATG SbfI 4421 CCTGCAGG CCTGCA^GG 4714 CCTGCAGG SexAI 2004 ACCTGGT A^CC[AT]GGT 4293 ACCTGGT SphI 1709 GCATGC GCATG^C 2724 GCATGC Sse8387I 4421 CCTGCAGG CCTGCA^GG 4714 CCTGCAGG XcmI 2507 CCAGCCCTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4162 CCAGCACACCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp718I AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI