##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI BamHI BbeI
BbrPI BsaHI BsiWI BspDI BspLU11I
BstBI BstEII BstPI ClaI CpoI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HindIII KasI
MfeI MunI NarI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 3937 TCCGGA T^CCGGA
AspI 4928 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4393 TGCGCA TGC^GCA
BcgI 452 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5057 TGATCA T^GATCA
BsaAI 509 CACGTA [CT]AC^GT[GA]
BsaBI 1617 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1493 GGTCTC GGTCTC(1/5)
BseAI 3937 TCCGGA T^CCGGA
BsmBI 64 GAGACG CGTCTC(1/5)
BsmI 2889 GAATGC GAATGC(1/-1)
Bsp1407I 3812 TGTACA T^GTACA
BspEI 3937 TCCGGA T^CCGGA
BsrGI 3812 TGTACA T^GTACA
BssSI 1723 CACGAG CACGAG(-5/-1)
Bst1107I 4824 GTATAC GTA^TAC
BstZ17I 4824 GTATAC GTA^TAC
DraI 5028 TTTAAA TTT^AAA
DraIII 1966 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
FbaI 5057 TGATCA T^GATCA
FspI 4393 TGCGCA TGC^GCA
HgaI 66 GACGC GACGC(5/10)
HpaI 4761 GTTAAC GTT^AAC
KspI 76 CCGCGG CCGC^GG
MamI 1617 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 67 ACGCGT A^CGCGT
MroI 3937 TCCGGA T^CCGGA
NaeI 1675 GCCGGC GCC^GGC
NgoMIV 1675 GCCGGC G^CCGGC
PaeR7I 873 CTCGAG C^TCGAG
PflFI 4928 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 371 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2334 GAGCTC GAGCT^C
SacII 76 CCGCGG CCGC^GG
ScaI 5082 AGTACT AGT^ACT
SspBI 3812 TGTACA T^GTACA
Tth111I 4928 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4533 TCTAGA T^CTAGA
XhoI 873 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 3489 CTTAAG C^TTAAG
5399 CTTAAG
AflIII 67 ACGCGT A^C[GA][CT]GT
508 ACACGT
AocI 562 CCTCAGG CC^T[ACGT]AGG
2409 CCTGAGG
AspHI 2334 GAGCTC G[AT]GC[AT]^C
3208 GTGCTC
BfrI 3489 CTTAAG C^TTAAG
5399 CTTAAG
BsiEI 20 CGGCCG CG[GA][CT]^CG
3587 CGGTCG
BsiHKAI 2334 GAGCTC G[AT]GC[AT]^C
3208 GTGCTC
BsrBI 2667 CCGCTC CCGCTC(-3/-3)
447 GAGCGG
BsrFI 1675 GCCGGC [GA]^CCGG[CT]
3585 GCCGGT
BssHII 81 GCGCGC G^CGCGC
83 GCGCGC
Bsu36I 562 CCTCAGG CC^T[ACGT]AGG
2409 CCTGAGG
Cfr10I 1675 GCCGGC [GA]^CCGG[CT]
3585 GCCGGT
DrdI 1980 GACCGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5130 GACAGAATGGTC
Eco81I 562 CCTCAGG CC^T[ACGT]AGG
2409 CCTGAGG
HincII 620 GTTGAC GT[CT]^[GA]AC
4761 GTTAAC
HindII 620 GTTGAC GT[CT]^[GA]AC
4761 GTTAAC
NdeI 4406 CATATG CA^TATG
5253 CATATG
SbfI 4421 CCTGCAGG CCTGCA^GG
4714 CCTGCAGG
SexAI 2004 ACCTGGT A^CC[AT]GGT
4293 ACCTGGT
SphI 1709 GCATGC GCATG^C
2724 GCATGC
Sse8387I 4421 CCTGCAGG CCTGCA^GG
4714 CCTGCAGG
XcmI 2507 CCAGCCCTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4162 CCAGCACACCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AluI AlwI AlwNI ApaI
ApoI Asp718I AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI