##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI ApaI AscI Asp718I
AvaI AviII AvrII BalI BanI
BbeI BbrPI BcgI BclI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrDI
BssSI BstBI BstXI CelII ClaI
CpoI DrdI EagI EarI EclXI
Eco47III Eco52I EcoRI EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3093 GTATAC GT^[AC][GT]AC
AflII 2359 CTTAAG C^TTAAG
AflIII 2930 ACATGT A^C[GA][CT]GT
AhdI 4485 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1521 GTGCAC G^TGCAC
ApaLI 1521 GTGCAC G^TGCAC
AspEI 4485 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1243 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3672 GTATCC GTATCC(6/5)
BcnI 86 CCCGG CC^[GC]GG
BfrI 2359 CTTAAG C^TTAAG
BsaI 596 GAGACC GGTCTC(1/5)
BsaWI 2407 TCCGGT [AT]^CCGG[AT]
BspHI 3882 TCATGA T^CATGA
BspLU11I 2930 ACATGT A^CATGT
BsrFI 4262 GCCGGT [GA]^CCGG[CT]
Bst1107I 3093 GTATAC GTA^TAC
BstEII 1859 GGTGACC G^GT[ACGT]ACC
BstPI 1859 GGTGACC G^GT[ACGT]ACC
BstZ17I 3093 GTATAC GTA^TAC
Cfr10I 4262 GCCGGT [GA]^CCGG[CT]
DsaI 2130 CCATGG C^C[GA][CT]GG
EaeI 88 CGGCCA [CT]^GGCC[GA]
Eam1105I 4485 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 45 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1859 GGTGACC G^GT[ACGT]ACC
MslI 1853 CATATAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 86 CCCGG CC^[GC]GG
NcoI 2130 CCATGG C^CATGG
PflFI 1243 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1047 CAGCTG CAG^CTG
RcaI 3882 TCATGA T^CATGA
SexAI 994 ACCTGGT A^CC[AT]GGT
TaqI 3177 TCGA T^CGA
Tth111I 1243 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3177 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AseI 2474 ATTAAT AT^TAAT
3197 ATTAAT
AsnI 2474 ATTAAT AT^TAAT
3197 ATTAAT
Asp700I 1715 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2822 GAAATAGTTC
BamHI 3747 GGATCC G^GATCC
4954 GGATCC
BanII 1607 GAGCCC G[GA]GC[CT]^C
2675 GAGCCC
BbsI 1249 GTCTTC GAAGAC(2/6)
3726 GTCTTC
BglII 1531 AGATCT A^GATCT
2232 AGATCT
BpuAI 1249 GTCTTC GAAGAC(2/6)
3726 GTCTTC
BsgI 29 GTGCAG GTGCAG(16/14)
794 GTGCAG
Bsp1407I 1180 TGTACA T^GTACA
4267 TGTACA
BsrGI 1180 TGTACA T^GTACA
4267 TGTACA
BssHII 3495 GCGCGC G^CGCGC
3497 GCGCGC
DraIII 1704 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3085 CACTAAGTG
EcoRV 1568 GATATC GAT^ATC
5048 GATATC
FauI 38 CCCGC CCCGC(4/6)
4229 CCCGC
HincII 2913 GTTAAC GT[CT]^[GA]AC
3853 GTTAAC
HindII 2913 GTTAAC GT[CT]^[GA]AC
3853 GTTAAC
HpaI 2913 GTTAAC GTT^AAC
3853 GTTAAC
MaeII 966 ACGT A^CGT
3128 ACGT
MspA1I 290 CCGCTG C[AC]G^C[GT]G
1047 CAGCTG
PshBI 2474 ATTAAT AT^TAAT
3197 ATTAAT
SpeI 818 ACTAGT A^CTAGT
1778 ACTAGT
SphI 1039 GCATGC GCATG^C
3491 GCATGC
SspBI 1180 TGTACA T^GTACA
4267 TGTACA
XcmI 2195 CCACCGTTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3576 CCAATATAAGACTGG
XmnI 1715 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2822 GAAATAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BbvCI BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII