##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AspEI AviII
BbeI BfrI BsaHI BsaWI BseAI
BsiEI BsiWI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
CpoI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HpaI KasI KspI MfeI
MluI MroI MunI NarI NotI
NruI NsiI NspV PacI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AseI 4165 ATTAAT AT^TAAT
AsnI 4165 ATTAAT AT^TAAT
BbsI 3259 GAAGAC GAAGAC(2/6)
BcgI 1674 GCAGGGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2598 TGATCA T^GATCA
BpuAI 3259 GAAGAC GAAGAC(2/6)
BsaBI 4336 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 401 CTGCAC GTGCAG(16/14)
BsmBI 665 CGTCTC CGTCTC(1/5)
BsmI 1069 GAATGC GAATGC(1/-1)
BspDI 548 ATCGAT AT^CGAT
BsrFI 263 GCCGGC [GA]^CCGG[CT]
BstEII 2933 GGTCACC G^GT[ACGT]ACC
BstPI 2933 GGTCACC G^GT[ACGT]ACC
Cfr10I 263 GCCGGC [GA]^CCGG[CT]
ClaI 548 ATCGAT AT^CGAT
DraI 2300 TTTAAA TTT^AAA
EcoO65I 2933 GGTCACC G^GT[ACGT]ACC
FbaI 2598 TGATCA T^GATCA
HgaI 664 GCGTC GACGC(5/10)
HincII 1127 GTCAAC GT[CT]^[GA]AC
HindII 1127 GTCAAC GT[CT]^[GA]AC
HindIII 1112 AAGCTT A^AGCTT
MamI 4336 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 263 GCCGGC GCC^GGC
NdeI 4588 CATATG CA^TATG
NgoMIV 263 GCCGGC G^CCGGC
NheI 3607 GCTAGC G^CTAGC
PaeR7I 3306 CTCGAG C^TCGAG
PshAI 3214 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4165 ATTAAT AT^TAAT
SapI 3507 GAAGAGC GCTCTTC(1/4)
SphI 2008 GCATGC GCATG^C
SwaI 2299 ATTTAAAT ATTT^AAAT
XbaI 2835 TCTAGA T^CTAGA
XhoI 3306 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 3022 CGCG CG^CG
3040 CGCG
Aor51HI 202 AGCGCT AGC^GCT
4253 AGCGCT
Asp700I 758 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2219 GAAGCAGTTC
AspI 1121 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2905 GACTCAGTC
BbrPI 2377 CACGTG CAC^GTG
3162 CACGTG
BciVI 19 GGATAC GTATCC(6/5)
3626 GGATAC
BcnI 840 CCCGG CC^[GC]GG
2955 CCCGG
BglII 2679 AGATCT A^GATCT
3718 AGATCT
BlpI 2349 GCTCAGC GC^T[ACGT]AGC
4044 GCTCAGC
Bpu1102I 2349 GCTCAGC GC^T[ACGT]AGC
4044 GCTCAGC
BsaI 3370 GAGACC GGTCTC(1/5)
2731 GGTCTC
BspLU11I 582 ACATGT A^CATGT
1152 ACATGT
BsrDI 652 CATTGC GCAATG(2/0)
2717 CATTGC
BstAPI 1989 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3946 GCAGTGAGTGC
BstUI 3022 CGCG CG^CG
3040 CGCG
CelII 2349 GCTCAGC GC^T[ACGT]AGC
4044 GCTCAGC
DraIII 1555 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2936 CACCCAGTG
DrdI 2200 GACAAATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACTGGGTGGTC
Eco47III 202 AGCGCT AGC^GCT
4253 AGCGCT
HaeII 202 AGCGCT [GA]GCGC^[CT]
4253 AGCGCT
MvnI 3022 CGCG CG^CG
3040 CGCG
NciI 840 CCCGG CC^[GC]GG
2955 CCCGG
PflFI 1121 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2905 GACTCAGTC
PmaCI 2377 CACGTG CAC^GTG
3162 CACGTG
PmlI 2377 CACGTG CAC^GTG
3162 CACGTG
PpuMI 396 GGGACCT [GA]G^G[AT]CC[CT]
3491 GGGACCC
SspI 1043 AATATT AAT^ATT
4162 AATATT
Tth111I 1121 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2905 GACTCAGTC
XmnI 758 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2219 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
Acc65I AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI Asp718I
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbvCI BbvI
BfaI BglI BlnI BmyI BpmI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII