##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccI AccII AccIII AflII AocI
ApaI AscI AseI AsnI AviII
AvrII BbeI BcgI BciVI BfrI
BlnI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstUI BstZ17I Bsu36I
ClaI CpoI DrdI EagI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
FseI FspI KasI KspI MluI
MroI MvnI NarI NheI NotI
NruI PacI PaeR7I PmeI PshBI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1792 GACGTC GACGT^C
Acc65I 2633 GGTACC G^GTACC
AclI 2323 AACGTT AA^CGTT
AgeI 2724 ACCGGT A^CCGGT
AhdI 1128 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3635 GTGCAC G^TGCAC
Aor51HI 471 AGCGCT AGC^GCT
ApaLI 3635 GTGCAC G^TGCAC
Asp718I 2633 GGTACC G^GTACC
AspEI 1128 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2508 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1179 GGATCC G^GATCC
BbrPI 3577 CACGTG CAC^GTG
BbvCI 1097 CCTCAGC CCTCAGC(-5/-2)
BlpI 1728 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1728 GCTGAGC GC^T[ACGT]AGC
BsaAI 3577 CACGTG [CT]AC^GT[GA]
BsaBI 2550 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1462 GAGACC GGTCTC(1/5)
BsaWI 2724 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3885 TGTACA T^GTACA
BsrGI 3885 TGTACA T^GTACA
BstBI 1307 TTCGAA TT^CGAA
BstEII 4416 GGTAACC G^GT[ACGT]ACC
BstPI 4416 GGTAACC G^GT[ACGT]ACC
CelII 1728 GCTGAGC GC^T[ACGT]AGC
DraIII 771 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1128 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 471 AGCGCT AGC^GCT
EcoO65I 4416 GGTAACC G^GT[ACGT]ACC
EcoT22I 2118 ATGCAT ATGCA^T
HaeII 471 AGCGCT [GA]GCGC^[CT]
HindIII 1249 AAGCTT A^AGCTT
KpnI 2633 GGTACC GGTAC^C
MamI 2550 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2904 CAATTG C^AATTG
MunI 2904 CAATTG C^AATTG
NcoI 4129 CCATGG C^CATGG
NsiI 2118 ATGCAT ATGCA^T
NspV 1307 TTCGAA TT^CGAA
PflFI 2508 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1011 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2724 ACCGGT A^CCGGT
PmaCI 3577 CACGTG CAC^GTG
PmlI 3577 CACGTG CAC^GTG
PshAI 2438 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2323 AACGTT AA^CGTT
SacI 2362 GAGCTC GAGCT^C
SfuI 1307 TTCGAA TT^CGAA
SmaI 401 CCCGGG CCC^GGG
SspBI 3885 TGTACA T^GTACA
SspI 3286 AATATT AAT^ATT
SwaI 2810 ATTTAAAT ATTT^AAAT
Tth111I 2508 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1011 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 401 CCCGGG C^CCGGG
XmaI 401 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 1792 GACGTC G[GA]^CG[CT]C
4016 GGCGTC
BclI 689 TGATCA T^GATCA
2732 TGATCA
BsaHI 1792 GACGTC G[GA]^CG[CT]C
4016 GGCGTC
BsrDI 2367 CATTGC GCAATG(2/0)
215 GCAATG
DraII 1023 AGGTCCT [GA]G^G[ACGT]CC[CT]
2310 AGGTCCC
EcoO109I 1023 AGGTCCT [GA]G^G[ACGT]CC[CT]
2310 AGGTCCC
FauI 2080 CCCGC CCCGC(4/6)
3995 CCCGC
FbaI 689 TGATCA T^GATCA
2732 TGATCA
Hin1I 1792 GACGTC G[GA]^CG[CT]C
4016 GGCGTC
HpaI 2326 GTTAAC GTT^AAC
2984 GTTAAC
NaeI 278 GCCGGC GCC^GGC
566 GCCGGC
NdeI 55 CATATG CA^TATG
3047 CATATG
NgoMIV 278 GCCGGC G^CCGGC
566 GCCGGC
PpuMI 1023 AGGTCCT [GA]G^G[AT]CC[CT]
2310 AGGTCCC
PvuII 1234 CAGCTG CAG^CTG
2215 CAGCTG
ScaI 1260 AGTACT AGT^ACT
2034 AGTACT
StuI 889 AGGCCT AGG^CCT
1352 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI