##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BbeI
BcgI BciVI BclI BfrI BglII
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2249 GACGTC GACGT^C
AspI 2249 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 499 CCTAGG C^CTAGG
BbrPI 1490 CACGTG CAC^GTG
BbsI 1130 GAAGAC GAAGAC(2/6)
BglI 2426 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 499 CCTAGG C^CTAGG
BpuAI 1130 GAAGAC GAAGAC(2/6)
BsaAI 1490 CACGTG [CT]AC^GT[GA]
BsiEI 1510 CGGCCG CG[GA][CT]^CG
BsmBI 474 CGTCTC CGTCTC(1/5)
Bsp1407I 2566 TGTACA T^GTACA
BspMI 1366 ACCTGC ACCTGC(4/8)
BsrDI 2227 CATTGC GCAATG(2/0)
BsrGI 2566 TGTACA T^GTACA
EagI 1510 CGGCCG C^GGCCG
EclXI 1510 CGGCCG C^GGCCG
Eco52I 1510 CGGCCG C^GGCCG
HaeII 2645 AGCGCC [GA]GCGC^[CT]
HincII 559 GTCAAC GT[CT]^[GA]AC
HindII 559 GTCAAC GT[CT]^[GA]AC
NaeI 1508 GCCGGC GCC^GGC
NgoMIV 1508 GCCGGC G^CCGGC
PflFI 2249 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1490 CACGTG CAC^GTG
PmlI 1490 CACGTG CAC^GTG
PpuMI 1974 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2289 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1841 GAGCTC GAGCT^C
SapI 1387 GAAGAGC GCTCTTC(1/4)
ScaI 251 AGTACT AGT^ACT
SmaI 1095 CCCGGG CCC^GGG
SspBI 2566 TGTACA T^GTACA
TfiI 1908 GATTC G^A[AT]TC
Tsp509I 96 AATT ^AATT
Tth111I 2249 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2178 CCATTGGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1095 CCCGGG C^CCGGG
XmaI 1095 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 79 GACGCC G[GA]^CG[CT]C
2249 GACGTC
Alw44I 1493 GTGCAC G^TGCAC
1613 GTGCAC
ApaLI 1493 GTGCAC G^TGCAC
1613 GTGCAC
AvaI 841 CCCGAG C^[CT]CG[GA]G
1095 CCCGGG
BamHI 1572 GGATCC G^GATCC
2170 GGATCC
BbvCI 1315 CCTCAGC CCTCAGC(-5/-2)
1886 GCTGAGG
BsaHI 79 GACGCC G[GA]^CG[CT]C
2249 GACGTC
BsmI 1182 GCATTC GAATGC(1/-1)
2024 GCATTC
BsoBI 841 CCCGAG C^[CT]CG[GA]G
1095 CCCGGG
BspHI 630 TCATGA T^CATGA
2355 TCATGA
BstAPI 718 GCACGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
958 GCAGCAGATGC
CfoI 320 GCGC GCG^C
2646 GCGC
DraIII 1753 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2301 CACTGGGTG
FauI 877 CCCGC CCCGC(4/6)
2602 GCGGG
HhaI 320 GCGC GCG^C
2646 GCGC
Hin1I 79 GACGCC G[GA]^CG[CT]C
2249 GACGTC
HinP1I 320 GCGC G^CGC
2646 GCGC
PvuII 908 CAGCTG CAG^CTG
968 CAGCTG
RcaI 630 TCATGA T^CATGA
2355 TCATGA
StuI 1062 AGGCCT AGG^CCT
2433 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XhoII