################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbeI BcgI BciVI BclI BfrI BglII BsaBI BsaI BsaWI BseAI BsgI BsiWI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 2249 GACGTC GACGT^C AspI 2249 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 499 CCTAGG C^CTAGG BbrPI 1490 CACGTG CAC^GTG BbsI 1130 GAAGAC GAAGAC(2/6) BglI 2426 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 499 CCTAGG C^CTAGG BpuAI 1130 GAAGAC GAAGAC(2/6) BsaAI 1490 CACGTG [CT]AC^GT[GA] BsiEI 1510 CGGCCG CG[GA][CT]^CG BsmBI 474 CGTCTC CGTCTC(1/5) Bsp1407I 2566 TGTACA T^GTACA BspMI 1366 ACCTGC ACCTGC(4/8) BsrDI 2227 CATTGC GCAATG(2/0) BsrGI 2566 TGTACA T^GTACA EagI 1510 CGGCCG C^GGCCG EclXI 1510 CGGCCG C^GGCCG Eco52I 1510 CGGCCG C^GGCCG HaeII 2645 AGCGCC [GA]GCGC^[CT] HincII 559 GTCAAC GT[CT]^[GA]AC HindII 559 GTCAAC GT[CT]^[GA]AC NaeI 1508 GCCGGC GCC^GGC NgoMIV 1508 GCCGGC G^CCGGC PflFI 2249 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1490 CACGTG CAC^GTG PmlI 1490 CACGTG CAC^GTG PpuMI 1974 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 2289 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1841 GAGCTC GAGCT^C SapI 1387 GAAGAGC GCTCTTC(1/4) ScaI 251 AGTACT AGT^ACT SmaI 1095 CCCGGG CCC^GGG SspBI 2566 TGTACA T^GTACA TfiI 1908 GATTC G^A[AT]TC Tsp509I 96 AATT ^AATT Tth111I 2249 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2178 CCATTGGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1095 CCCGGG C^CCGGG XmaI 1095 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 79 GACGCC G[GA]^CG[CT]C 2249 GACGTC Alw44I 1493 GTGCAC G^TGCAC 1613 GTGCAC ApaLI 1493 GTGCAC G^TGCAC 1613 GTGCAC AvaI 841 CCCGAG C^[CT]CG[GA]G 1095 CCCGGG BamHI 1572 GGATCC G^GATCC 2170 GGATCC BbvCI 1315 CCTCAGC CCTCAGC(-5/-2) 1886 GCTGAGG BsaHI 79 GACGCC G[GA]^CG[CT]C 2249 GACGTC BsmI 1182 GCATTC GAATGC(1/-1) 2024 GCATTC BsoBI 841 CCCGAG C^[CT]CG[GA]G 1095 CCCGGG BspHI 630 TCATGA T^CATGA 2355 TCATGA BstAPI 718 GCACGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 958 GCAGCAGATGC CfoI 320 GCGC GCG^C 2646 GCGC DraIII 1753 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2301 CACTGGGTG FauI 877 CCCGC CCCGC(4/6) 2602 GCGGG HhaI 320 GCGC GCG^C 2646 GCGC Hin1I 79 GACGCC G[GA]^CG[CT]C 2249 GACGTC HinP1I 320 GCGC G^CGC 2646 GCGC PvuII 908 CAGCTG CAG^CTG 968 CAGCTG RcaI 630 TCATGA T^CATGA 2355 TCATGA StuI 1062 AGGCCT AGG^CCT 2433 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XhoII