##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccII AccIII AgeI AocI
Aor51HI ApaI AscI Asp718I AviII
AvrII BamHI BbeI BbrPI BlnI
BsaBI BseAI BsiWI BspEI BsrBI
BssHII BstBI BstUI Bsu36I CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FauI FseI FspI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 806 GACGTC GACGT^C
AclI 2885 AACGTT AA^CGTT
AcyI 806 GACGTC G[GA]^CG[CT]C
Alw44I 1714 GTGCAC G^TGCAC
ApaLI 1714 GTGCAC G^TGCAC
Asp700I 23 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3733 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3466 GCTGAGG CCTCAGC(-5/-2)
BcgI 2833 GCAACTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1215 CCCGG CC^[GC]GG
BsaAI 1781 TACGTG [CT]AC^GT[GA]
BsaHI 806 GACGTC G[GA]^CG[CT]C
BsiEI 2410 CGACCG CG[GA][CT]^CG
BsmBI 906 CGTCTC CGTCTC(1/5)
BspDI 2322 ATCGAT AT^CGAT
Bst1107I 1819 GTATAC GTA^TAC
BstEII 2183 GGTGACC G^GT[ACGT]ACC
BstPI 2183 GGTGACC G^GT[ACGT]ACC
BstZ17I 1819 GTATAC GTA^TAC
CfoI 462 GCGC GCG^C
ClaI 2322 ATCGAT AT^CGAT
DrdI 806 GACGTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2183 GGTGACC G^GT[ACGT]ACC
EcoRI 5201 GAATTC G^AATTC
EcoT22I 2101 ATGCAT ATGCA^T
HaeII 461 AGCGCC [GA]GCGC^[CT]
HhaI 462 GCGC GCG^C
Hin1I 806 GACGTC G[GA]^CG[CT]C
HinP1I 462 GCGC G^CGC
HindIII 3582 AAGCTT A^AGCTT
NciI 1215 CCCGG CC^[GC]GG
NsiI 2101 ATGCAT ATGCA^T
PaeR7I 3018 CTCGAG C^TCGAG
PflFI 3733 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2885 AACGTT AA^CGTT
ScaI 1589 AGTACT AGT^ACT
Tth111I 3733 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3018 CTCGAG C^TCGAG
XmnI 23 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1819 GTATAC GT^[AC][GT]AC
3231 GTCTAC
AflII 530 CTTAAG C^TTAAG
1397 CTTAAG
AflIII 105 ACATGT A^C[GA][CT]GT
2472 ACATGT
AhdI 683 GACCGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
896 GACACACAGTC
AspEI 683 GACCGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
896 GACACACAGTC
AvaI 1593 CTCGGG C^[CT]CG[GA]G
3018 CTCGAG
BclI 1063 TGATCA T^GATCA
3310 TGATCA
BfrI 530 CTTAAG C^TTAAG
1397 CTTAAG
BglII 1104 AGATCT A^GATCT
4042 AGATCT
BlpI 4458 GCTCAGC GC^T[ACGT]AGC
5471 GCTCAGC
Bpu1102I 4458 GCTCAGC GC^T[ACGT]AGC
5471 GCTCAGC
BsaWI 882 TCCGGT [AT]^CCGG[AT]
3138 ACCGGA
BsoBI 1593 CTCGGG C^[CT]CG[GA]G
3018 CTCGAG
BspHI 718 TCATGA T^CATGA
5553 TCATGA
BspLU11I 105 ACATGT A^CATGT
2472 ACATGT
BsrFI 474 ACCGGC [GA]^CCGG[CT]
2255 ACCGGC
BssSI 5527 CACGAG CACGAG(-5/-1)
1282 CTCGTG
BstXI 2955 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCAGTGCCCTGG
CelII 4458 GCTCAGC GC^T[ACGT]AGC
5471 GCTCAGC
Cfr10I 474 ACCGGC [GA]^CCGG[CT]
2255 ACCGGC
DraI 4141 TTTAAA TTT^AAA
4849 TTTAAA
DraIII 1683 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4019 CACTAAGTG
Eam1105I 683 GACCGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
896 GACACACAGTC
FbaI 1063 TGATCA T^GATCA
3310 TGATCA
HgaI 1638 GCGTC GACGC(5/10)
3292 GCGTC
HpaI 190 GTTAAC GTT^AAC
4811 GTTAAC
MfeI 3923 CAATTG C^AATTG
4050 CAATTG
MunI 3923 CAATTG C^AATTG
4050 CAATTG
NcoI 1454 CCATGG C^CATGG
5290 CCATGG
RcaI 718 TCATGA T^CATGA
5553 TCATGA
XbaI 2032 TCTAGA T^CTAGA
5028 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII