################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccII AccIII AgeI AocI Aor51HI ApaI AscI Asp718I AviII AvrII BamHI BbeI BbrPI BlnI BsaBI BseAI BsiWI BspEI BsrBI BssHII BstBI BstUI Bsu36I CpoI EagI EclXI Eco47III Eco52I Eco81I EcoRV FauI FseI FspI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 806 GACGTC GACGT^C AclI 2885 AACGTT AA^CGTT AcyI 806 GACGTC G[GA]^CG[CT]C Alw44I 1714 GTGCAC G^TGCAC ApaLI 1714 GTGCAC G^TGCAC Asp700I 23 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3733 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 3466 GCTGAGG CCTCAGC(-5/-2) BcgI 2833 GCAACTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1215 CCCGG CC^[GC]GG BsaAI 1781 TACGTG [CT]AC^GT[GA] BsaHI 806 GACGTC G[GA]^CG[CT]C BsiEI 2410 CGACCG CG[GA][CT]^CG BsmBI 906 CGTCTC CGTCTC(1/5) BspDI 2322 ATCGAT AT^CGAT Bst1107I 1819 GTATAC GTA^TAC BstEII 2183 GGTGACC G^GT[ACGT]ACC BstPI 2183 GGTGACC G^GT[ACGT]ACC BstZ17I 1819 GTATAC GTA^TAC CfoI 462 GCGC GCG^C ClaI 2322 ATCGAT AT^CGAT DrdI 806 GACGTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2183 GGTGACC G^GT[ACGT]ACC EcoRI 5201 GAATTC G^AATTC EcoT22I 2101 ATGCAT ATGCA^T HaeII 461 AGCGCC [GA]GCGC^[CT] HhaI 462 GCGC GCG^C Hin1I 806 GACGTC G[GA]^CG[CT]C HinP1I 462 GCGC G^CGC HindIII 3582 AAGCTT A^AGCTT NciI 1215 CCCGG CC^[GC]GG NsiI 2101 ATGCAT ATGCA^T PaeR7I 3018 CTCGAG C^TCGAG PflFI 3733 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2885 AACGTT AA^CGTT ScaI 1589 AGTACT AGT^ACT Tth111I 3733 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3018 CTCGAG C^TCGAG XmnI 23 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 1819 GTATAC GT^[AC][GT]AC 3231 GTCTAC AflII 530 CTTAAG C^TTAAG 1397 CTTAAG AflIII 105 ACATGT A^C[GA][CT]GT 2472 ACATGT AhdI 683 GACCGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 896 GACACACAGTC AspEI 683 GACCGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 896 GACACACAGTC AvaI 1593 CTCGGG C^[CT]CG[GA]G 3018 CTCGAG BclI 1063 TGATCA T^GATCA 3310 TGATCA BfrI 530 CTTAAG C^TTAAG 1397 CTTAAG BglII 1104 AGATCT A^GATCT 4042 AGATCT BlpI 4458 GCTCAGC GC^T[ACGT]AGC 5471 GCTCAGC Bpu1102I 4458 GCTCAGC GC^T[ACGT]AGC 5471 GCTCAGC BsaWI 882 TCCGGT [AT]^CCGG[AT] 3138 ACCGGA BsoBI 1593 CTCGGG C^[CT]CG[GA]G 3018 CTCGAG BspHI 718 TCATGA T^CATGA 5553 TCATGA BspLU11I 105 ACATGT A^CATGT 2472 ACATGT BsrFI 474 ACCGGC [GA]^CCGG[CT] 2255 ACCGGC BssSI 5527 CACGAG CACGAG(-5/-1) 1282 CTCGTG BstXI 2955 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3069 CCAGTGCCCTGG CelII 4458 GCTCAGC GC^T[ACGT]AGC 5471 GCTCAGC Cfr10I 474 ACCGGC [GA]^CCGG[CT] 2255 ACCGGC DraI 4141 TTTAAA TTT^AAA 4849 TTTAAA DraIII 1683 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 4019 CACTAAGTG Eam1105I 683 GACCGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 896 GACACACAGTC FbaI 1063 TGATCA T^GATCA 3310 TGATCA HgaI 1638 GCGTC GACGC(5/10) 3292 GCGTC HpaI 190 GTTAAC GTT^AAC 4811 GTTAAC MfeI 3923 CAATTG C^AATTG 4050 CAATTG MunI 3923 CAATTG C^AATTG 4050 CAATTG NcoI 1454 CCATGG C^CATGG 5290 CCATGG RcaI 718 TCATGA T^CATGA 5553 TCATGA XbaI 2032 TCTAGA T^CTAGA 5028 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII