##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflIII AocI AscI
Asp718I AviII BalI BamHI BbeI
BbrPI BglI BlpI Bpu1102I BsiEI
BsiWI BspDI BspLU11I BssHII BstBI
Bsu36I CelII ClaI CpoI DraIII
DrdI EaeI EagI EclXI Eco52I
Eco81I EcoRV FseI FspI HpaI
KasI KpnI KspI MluI MluNI
MscI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1908 GACGTC GACGT^C
AccI 3665 GTATAC GT^[AC][GT]AC
AccII 27 CGCG CG^CG
AccIII 1034 TCCGGA T^CCGGA
AgeI 538 ACCGGT A^CCGGT
AhdI 2192 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1362 GGGCCC GGGCC^C
AseI 4701 ATTAAT AT^TAAT
AsnI 4701 ATTAAT AT^TAAT
Asp700I 3651 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2192 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 79 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1537 GCTGAGG CCTCAGC(-5/-2)
BcgI 3556 GCACTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1773 GGATAC GTATCC(6/5)
BseAI 1034 TCCGGA T^CCGGA
BsmBI 310 GAGACG CGTCTC(1/5)
BspEI 1034 TCCGGA T^CCGGA
BsrBI 1587 CCGCTC CCGCTC(-3/-3)
BsrFI 538 ACCGGT [GA]^CCGG[CT]
BssSI 231 CACGAG CACGAG(-5/-1)
Bst1107I 3665 GTATAC GTA^TAC
BstAPI 4025 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 27 CGCG CG^CG
BstXI 4433 CCATGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3665 GTATAC GTA^TAC
Cfr10I 538 ACCGGT [GA]^CCGG[CT]
DsaI 4829 CCATGG C^C[GA][CT]GG
Eam1105I 2192 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1333 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1288 GAATTC G^AATTC
FauI 1874 CCCGC CCCGC(4/6)
MroI 1034 TCCGGA T^CCGGA
MvnI 27 CGCG CG^CG
NcoI 4829 CCATGG C^CATGG
NdeI 5503 CATATG CA^TATG
PflFI 79 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4161 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 538 ACCGGT A^CCGGT
PshAI 5244 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4701 ATTAAT AT^TAAT
ScaI 5210 AGTACT AGT^ACT
SpeI 3677 ACTAGT A^CTAGT
SphI 4582 GCATGC GCATG^C
StuI 4132 AGGCCT AGG^CCT
Tth111I 79 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4161 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2111 CCATTTAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3651 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1271 GACGCC G[GA]^CG[CT]C
1908 GACGTC
AflII 4148 CTTAAG C^TTAAG
5537 CTTAAG
Alw44I 3395 GTGCAC G^TGCAC
5055 GTGCAC
Aor51HI 3432 AGCGCT AGC^GCT
4544 AGCGCT
ApaLI 3395 GTGCAC G^TGCAC
5055 GTGCAC
AvrII 2795 CCTAGG C^CTAGG
4135 CCTAGG
BanI 2733 GGCACC G^G[CT][GA]CC
5415 GGCACC
BclI 2904 TGATCA T^GATCA
5205 TGATCA
BcnI 2249 CCCGG CC^[GC]GG
4088 CCCGG
BfrI 4148 CTTAAG C^TTAAG
5537 CTTAAG
BlnI 2795 CCTAGG C^CTAGG
4135 CCTAGG
BsaAI 2900 TACGTG [CT]AC^GT[GA]
4677 CACGTA
BsaHI 1271 GACGCC G[GA]^CG[CT]C
1908 GACGTC
BsaI 177 GGTCTC GGTCTC(1/5)
2252 GGTCTC
Bsp1407I 3880 TGTACA T^GTACA
4797 TGTACA
BsrGI 3880 TGTACA T^GTACA
4797 TGTACA
BstEII 1990 GGTAACC G^GT[ACGT]ACC
3018 GGTTACC
BstPI 1990 GGTAACC G^GT[ACGT]ACC
3018 GGTTACC
Eco47III 3432 AGCGCT AGC^GCT
4544 AGCGCT
EcoO65I 1990 GGTAACC G^GT[ACGT]ACC
3018 GGTTACC
EcoT22I 1218 ATGCAT ATGCA^T
4023 ATGCAT
FbaI 2904 TGATCA T^GATCA
5205 TGATCA
HaeII 3432 AGCGCT [GA]GCGC^[CT]
4544 AGCGCT
HgaI 102 GACGC GACGC(5/10)
1271 GACGC
Hin1I 1271 GACGCC G[GA]^CG[CT]C
1908 GACGTC
HindIII 523 AAGCTT A^AGCTT
5534 AAGCTT
NciI 2249 CCCGG CC^[GC]GG
4088 CCCGG
NsiI 1218 ATGCAT ATGCA^T
4023 ATGCAT
NspI 1984 GCATGT [GA]CATG^[CT]
4582 GCATGC
PaeR7I 252 CTCGAG C^TCGAG
3400 CTCGAG
SacI 255 GAGCTC GAGCT^C
1254 GAGCTC
SspBI 3880 TGTACA T^GTACA
4797 TGTACA
XhoI 252 CTCGAG C^TCGAG
3400 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII