################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AclI AflIII AocI AscI Asp718I AviII BalI BamHI BbeI BbrPI BglI BlpI Bpu1102I BsiEI BsiWI BspDI BspLU11I BssHII BstBI Bsu36I CelII ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco52I Eco81I EcoRV FseI FspI HpaI KasI KpnI KspI MluI MluNI MscI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1908 GACGTC GACGT^C AccI 3665 GTATAC GT^[AC][GT]AC AccII 27 CGCG CG^CG AccIII 1034 TCCGGA T^CCGGA AgeI 538 ACCGGT A^CCGGT AhdI 2192 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1362 GGGCCC GGGCC^C AseI 4701 ATTAAT AT^TAAT AsnI 4701 ATTAAT AT^TAAT Asp700I 3651 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2192 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 79 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1537 GCTGAGG CCTCAGC(-5/-2) BcgI 3556 GCACTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1773 GGATAC GTATCC(6/5) BseAI 1034 TCCGGA T^CCGGA BsmBI 310 GAGACG CGTCTC(1/5) BspEI 1034 TCCGGA T^CCGGA BsrBI 1587 CCGCTC CCGCTC(-3/-3) BsrFI 538 ACCGGT [GA]^CCGG[CT] BssSI 231 CACGAG CACGAG(-5/-1) Bst1107I 3665 GTATAC GTA^TAC BstAPI 4025 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 27 CGCG CG^CG BstXI 4433 CCATGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3665 GTATAC GTA^TAC Cfr10I 538 ACCGGT [GA]^CCGG[CT] DsaI 4829 CCATGG C^C[GA][CT]GG Eam1105I 2192 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1333 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1288 GAATTC G^AATTC FauI 1874 CCCGC CCCGC(4/6) MroI 1034 TCCGGA T^CCGGA MvnI 27 CGCG CG^CG NcoI 4829 CCATGG C^CATGG NdeI 5503 CATATG CA^TATG PflFI 79 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4161 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 538 ACCGGT A^CCGGT PshAI 5244 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4701 ATTAAT AT^TAAT ScaI 5210 AGTACT AGT^ACT SpeI 3677 ACTAGT A^CTAGT SphI 4582 GCATGC GCATG^C StuI 4132 AGGCCT AGG^CCT Tth111I 79 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4161 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2111 CCATTTAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 3651 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 1271 GACGCC G[GA]^CG[CT]C 1908 GACGTC AflII 4148 CTTAAG C^TTAAG 5537 CTTAAG Alw44I 3395 GTGCAC G^TGCAC 5055 GTGCAC Aor51HI 3432 AGCGCT AGC^GCT 4544 AGCGCT ApaLI 3395 GTGCAC G^TGCAC 5055 GTGCAC AvrII 2795 CCTAGG C^CTAGG 4135 CCTAGG BanI 2733 GGCACC G^G[CT][GA]CC 5415 GGCACC BclI 2904 TGATCA T^GATCA 5205 TGATCA BcnI 2249 CCCGG CC^[GC]GG 4088 CCCGG BfrI 4148 CTTAAG C^TTAAG 5537 CTTAAG BlnI 2795 CCTAGG C^CTAGG 4135 CCTAGG BsaAI 2900 TACGTG [CT]AC^GT[GA] 4677 CACGTA BsaHI 1271 GACGCC G[GA]^CG[CT]C 1908 GACGTC BsaI 177 GGTCTC GGTCTC(1/5) 2252 GGTCTC Bsp1407I 3880 TGTACA T^GTACA 4797 TGTACA BsrGI 3880 TGTACA T^GTACA 4797 TGTACA BstEII 1990 GGTAACC G^GT[ACGT]ACC 3018 GGTTACC BstPI 1990 GGTAACC G^GT[ACGT]ACC 3018 GGTTACC Eco47III 3432 AGCGCT AGC^GCT 4544 AGCGCT EcoO65I 1990 GGTAACC G^GT[ACGT]ACC 3018 GGTTACC EcoT22I 1218 ATGCAT ATGCA^T 4023 ATGCAT FbaI 2904 TGATCA T^GATCA 5205 TGATCA HaeII 3432 AGCGCT [GA]GCGC^[CT] 4544 AGCGCT HgaI 102 GACGC GACGC(5/10) 1271 GACGC Hin1I 1271 GACGCC G[GA]^CG[CT]C 1908 GACGTC HindIII 523 AAGCTT A^AGCTT 5534 AAGCTT NciI 2249 CCCGG CC^[GC]GG 4088 CCCGG NsiI 1218 ATGCAT ATGCA^T 4023 ATGCAT NspI 1984 GCATGT [GA]CATG^[CT] 4582 GCATGC PaeR7I 252 CTCGAG C^TCGAG 3400 CTCGAG SacI 255 GAGCTC GAGCT^C 1254 GAGCTC SspBI 3880 TGTACA T^GTACA 4797 TGTACA XhoI 252 CTCGAG C^TCGAG 3400 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII