##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccIII AcyI Alw44I
ApaLI AscI Asp718I AspI AviII
BbeI BbrPI BsaAI BsaHI BseAI
BsiWI BsmBI BspDI BspEI BstBI
ClaI DrdI FseI FspI HgaI
Hin1I HpaI KasI KpnI MluI
MroI NarI NheI NruI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI SacI SalI SbfI
SexAI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I Tth111I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 4296 AACGTT AA^CGTT
AhdI 5870 GACTTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3878 GGGCCC GGGCC^C
AspEI 5870 GACTTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 253 CCCGGG C^[CT]CG[GA]G
BamHI 3491 GGATCC G^GATCC
BanI 2400 GGTGCC G^G[CT][GA]CC
BbvCI 1596 CCTCAGC CCTCAGC(-5/-2)
BciVI 5009 GGATAC GTATCC(6/5)
BclI 5231 TGATCA T^GATCA
BsaBI 4391 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 253 CCCGGG C^[CT]CG[GA]G
BspHI 6031 TCATGA T^CATGA
BsrBI 573 CCGCTC CCGCTC(-3/-3)
BsrDI 4897 CATTGC GCAATG(2/0)
BssHII 115 GCGCGC G^CGCGC
BssSI 4665 CACGAG CACGAG(-5/-1)
Bst1107I 3481 GTATAC GTA^TAC
BstEII 3500 GGTAACC G^GT[ACGT]ACC
BstPI 3500 GGTAACC G^GT[ACGT]ACC
BstXI 1836 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3481 GTATAC GTA^TAC
CpoI 320 CGGACCG CG^G[AT]CCG
DraIII 1282 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 168 CGGCCG C^GGCCG
Eam1105I 5870 GACTTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 168 CGGCCG C^GGCCG
Eco52I 168 CGGCCG C^GGCCG
EcoO65I 3500 GGTAACC G^GT[ACGT]ACC
EcoRV 2352 GATATC GAT^ATC
FbaI 5231 TGATCA T^GATCA
MamI 4391 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 374 GCCGGC GCC^GGC
NgoMIV 374 GCCGGC G^CCGGC
NotI 167 GCGGCCGC GC^GGCCGC
PpuMI 596 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 3424 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4296 AACGTT AA^CGTT
PvuI 79 CGATCG CGAT^CG
PvuII 1859 CAGCTG CAG^CTG
RcaI 6031 TCATGA T^CATGA
RsrII 320 CGGACCG CG^G[AT]CCG
SapI 1578 GCTCTTC GCTCTTC(1/4)
SgrAI 208 CACCGGTG C[GA]^CCGG[CT]G
SmaI 253 CCCGGG CCC^GGG
StuI 3123 AGGCCT AGG^CCT
SwaI 3029 ATTTAAAT ATTT^AAAT
XmaCI 253 CCCGGG C^CCGGG
XmaI 253 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflII 4400 CTTAAG C^TTAAG
5575 CTTAAG
AlwNI 1273 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1949 CAGCACCTG
Aor51HI 735 AGCGCT AGC^GCT
3777 AGCGCT
AvrII 1976 CCTAGG C^CTAGG
3379 CCTAGG
BalI 541 TGGCCA TGG^CCA
1300 TGGCCA
BcgI 4667 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1805 GCAGATGTGTCG
BfrI 4400 CTTAAG C^TTAAG
5575 CTTAAG
BglI 138 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1401 GCCTAGAAGGC
BlnI 1976 CCTAGG C^CTAGG
3379 CCTAGG
BsgI 1279 CTGCAC GTGCAG(16/14)
1966 CTGCAC
BsiEI 79 CGATCG CG[GA][CT]^CG
168 CGGCCG
Bsp1407I 1695 TGTACA T^GTACA
2566 TGTACA
BspMI 800 ACCTGC ACCTGC(4/8)
232 GCAGGT
BsrGI 1695 TGTACA T^GTACA
2566 TGTACA
BstAPI 1281 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1356 GCATCAACTGC
Eco47III 735 AGCGCT AGC^GCT
3777 AGCGCT
EcoNI 2179 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2532 CCTCAAAGAGG
HaeII 735 AGCGCT [GA]GCGC^[CT]
3777 AGCGCT
HincII 2593 GTCAAC GT[CT]^[GA]AC
4857 GTCAAC
HindII 2593 GTCAAC GT[CT]^[GA]AC
4857 GTCAAC
HindIII 2477 AAGCTT A^AGCTT
5850 AAGCTT
KspI 294 CCGCGG CCGC^GG
317 CCGCGG
MaeII 3425 ACGT A^CGT
4297 ACGT
MfeI 1165 CAATTG C^AATTG
2892 CAATTG
MluNI 541 TGGCCA TGG^CCA
1300 TGGCCA
MscI 541 TGGCCA TGG^CCA
1300 TGGCCA
MunI 1165 CAATTG C^AATTG
2892 CAATTG
NcoI 2483 CCATGG C^CATGG
3874 CCATGG
PstI 856 CTGCAG CTGCA^G
1556 CTGCAG
SacII 294 CCGCGG CCGC^GG
317 CCGCGG
ScaI 1535 AGTACT AGT^ACT
3279 AGTACT
SmlI 4400 CTTAAG C^T[CT][GA]AG
5575 CTTAAG
SspBI 1695 TGTACA T^GTACA
2566 TGTACA
XbaI 2644 TCTAGA T^CTAGA
3944 TCTAGA
XcmI 1207 CCAATGTAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3495 CCATAGGTAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AgeI AluI AlwI AocI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PinAI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI