##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AocI Aor51HI ApaI AscI
AvrII BbeI BbrPI BbvCI BclI
BcnI BlnI BsaAI BsaHI BsaWI
BseAI BsiEI BsmBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV FbaI FseI HaeII HgaI
Hin1I KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NgoMIV NotI NruI NspV
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 4312 GGTACC G^GTACC
AflII 142 CTTAAG C^TTAAG
AflIII 3126 ACATGT A^C[GA][CT]GT
Alw44I 3578 GTGCAC G^TGCAC
ApaLI 3578 GTGCAC G^TGCAC
Asp718I 4312 GGTACC G^GTACC
AspI 809 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 5215 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 142 CTTAAG C^TTAAG
BglI 2495 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3984 AGATCT A^GATCT
BlpI 3103 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3103 GCTGAGC GC^T[ACGT]AGC
BsaBI 6374 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 3557 CGTACG C^GTACG
BspLU11I 3126 ACATGT A^CATGT
BsrDI 5992 CATTGC GCAATG(2/0)
BssSI 2563 CACGAG CACGAG(-5/-1)
BstEII 3368 GGTGACC G^GT[ACGT]ACC
BstPI 3368 GGTGACC G^GT[ACGT]ACC
CelII 3103 GCTGAGC GC^T[ACGT]AGC
EcoO65I 3368 GGTGACC G^GT[ACGT]ACC
FauI 4627 GCGGG CCCGC(4/6)
HpaI 760 GTTAAC GTT^AAC
KpnI 4312 GGTACC GGTAC^C
MamI 6374 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2856 CCATGG C^CATGG
NheI 568 GCTAGC G^CTAGC
PacI 441 TTAATTAA TTAAT^TAA
PaeR7I 6325 CTCGAG C^TCGAG
PflFI 809 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4321 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 5147 GAGCTC GAGCT^C
SexAI 2931 ACCTGGT A^CC[AT]GGT
StuI 861 AGGCCT AGG^CCT
SwaI 1536 ATTTAAAT ATTT^AAAT
Tth111I 809 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5942 TCTAGA T^CTAGA
XhoI 6325 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 806 GTAGAC GT^[AC][GT]AC
2594 GTCTAC
AciI 2162 CCGC CCGC(-3/-1)
4627 GCGG
AhdI 2357 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5051 GACTTTCAGTC
AspEI 2357 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5051 GACTTTCAGTC
AviII 795 TGCGCA TGC^GCA
4352 TGCGCA
BalI 2853 TGGCCA TGG^CCA
3361 TGGCCA
BciVI 940 GGATAC GTATCC(6/5)
5747 GTATCC
BstAPI 4618 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5679 GCAGGGACTGC
DraIII 3540 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5798 CACCATGTG
DrdI 955 GACTCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3824 GACAAATTTGTC
DsaI 2856 CCATGG C^C[GA][CT]GG
6369 CCACGG
EaeI 2853 TGGCCA [CT]^GGCC[GA]
3361 TGGCCA
Eam1105I 2357 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5051 GACTTTCAGTC
EcoT22I 1530 ATGCAT ATGCA^T
3566 ATGCAT
FspI 795 TGCGCA TGC^GCA
4352 TGCGCA
HapII 395 CCGG C^CGG
5144 CCGG
HpaII 395 CCGG C^CGG
5144 CCGG
MluNI 2853 TGGCCA TGG^CCA
3361 TGGCCA
MscI 2853 TGGCCA TGG^CCA
3361 TGGCCA
MspI 395 CCGG C^CGG
5144 CCGG
NsiI 1530 ATGCAT ATGCA^T
3566 ATGCAT
SapI 5352 GAAGAGC GCTCTTC(1/4)
1315 GCTCTTC
SphI 3564 GCATGC GCATG^C
6318 GCATGC
SspI 11 AATATT AAT^ATT
1522 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI