################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AocI Aor51HI ApaI AscI AvrII BbeI BbrPI BbvCI BclI BcnI BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV FbaI FseI HaeII HgaI Hin1I KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NotI NruI NspV PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 4312 GGTACC G^GTACC AflII 142 CTTAAG C^TTAAG AflIII 3126 ACATGT A^C[GA][CT]GT Alw44I 3578 GTGCAC G^TGCAC ApaLI 3578 GTGCAC G^TGCAC Asp718I 4312 GGTACC G^GTACC AspI 809 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 5215 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 142 CTTAAG C^TTAAG BglI 2495 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3984 AGATCT A^GATCT BlpI 3103 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3103 GCTGAGC GC^T[ACGT]AGC BsaBI 6374 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 3557 CGTACG C^GTACG BspLU11I 3126 ACATGT A^CATGT BsrDI 5992 CATTGC GCAATG(2/0) BssSI 2563 CACGAG CACGAG(-5/-1) BstEII 3368 GGTGACC G^GT[ACGT]ACC BstPI 3368 GGTGACC G^GT[ACGT]ACC CelII 3103 GCTGAGC GC^T[ACGT]AGC EcoO65I 3368 GGTGACC G^GT[ACGT]ACC FauI 4627 GCGGG CCCGC(4/6) HpaI 760 GTTAAC GTT^AAC KpnI 4312 GGTACC GGTAC^C MamI 6374 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 2856 CCATGG C^CATGG NheI 568 GCTAGC G^CTAGC PacI 441 TTAATTAA TTAAT^TAA PaeR7I 6325 CTCGAG C^TCGAG PflFI 809 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4321 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 5147 GAGCTC GAGCT^C SexAI 2931 ACCTGGT A^CC[AT]GGT StuI 861 AGGCCT AGG^CCT SwaI 1536 ATTTAAAT ATTT^AAAT Tth111I 809 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5942 TCTAGA T^CTAGA XhoI 6325 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 806 GTAGAC GT^[AC][GT]AC 2594 GTCTAC AciI 2162 CCGC CCGC(-3/-1) 4627 GCGG AhdI 2357 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5051 GACTTTCAGTC AspEI 2357 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5051 GACTTTCAGTC AviII 795 TGCGCA TGC^GCA 4352 TGCGCA BalI 2853 TGGCCA TGG^CCA 3361 TGGCCA BciVI 940 GGATAC GTATCC(6/5) 5747 GTATCC BstAPI 4618 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5679 GCAGGGACTGC DraIII 3540 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 5798 CACCATGTG DrdI 955 GACTCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3824 GACAAATTTGTC DsaI 2856 CCATGG C^C[GA][CT]GG 6369 CCACGG EaeI 2853 TGGCCA [CT]^GGCC[GA] 3361 TGGCCA Eam1105I 2357 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5051 GACTTTCAGTC EcoT22I 1530 ATGCAT ATGCA^T 3566 ATGCAT FspI 795 TGCGCA TGC^GCA 4352 TGCGCA HapII 395 CCGG C^CGG 5144 CCGG HpaII 395 CCGG C^CGG 5144 CCGG MluNI 2853 TGGCCA TGG^CCA 3361 TGGCCA MscI 2853 TGGCCA TGG^CCA 3361 TGGCCA MspI 395 CCGG C^CGG 5144 CCGG NsiI 1530 ATGCAT ATGCA^T 3566 ATGCAT SapI 5352 GAAGAGC GCTCTTC(1/4) 1315 GCTCTTC SphI 3564 GCATGC GCATG^C 6318 GCATGC SspI 11 AATATT AAT^ATT 1522 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI