##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII Aor51HI AscI AseI AsnI
Asp700I AspI AviII BbeI BbrPI
BclI BfrI BsaHI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eco47III EcoRI FbaI
FseI FspI Hin1I KasI MluI
MroI NaeI NarI NdeI NgoMIV
NruI NspV PacI PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 882 GGTACC G^GTACC
AgeI 1050 ACCGGT A^CCGGT
Asp718I 882 GGTACC G^GTACC
BcgI 3202 GCAACAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 70 AGATCT A^GATCT
BsaBI 65 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 209 CGGCCG CG[GA][CT]^CG
BspLU11I 3086 ACATGT A^CATGT
BsrDI 870 GCAATG GCAATG(2/0)
BsrFI 1050 ACCGGT [GA]^CCGG[CT]
BssHII 114 GCGCGC G^CGCGC
BssSI 1200 CTCGTG CACGAG(-5/-1)
Cfr10I 1050 ACCGGT [GA]^CCGG[CT]
EagI 209 CGGCCG C^GGCCG
EclXI 209 CGGCCG C^GGCCG
Eco52I 209 CGGCCG C^GGCCG
EcoRV 5156 GATATC GAT^ATC
FauI 983 CCCGC CCCGC(4/6)
KpnI 882 GGTACC GGTAC^C
MamI 65 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 913 CAATTG C^AATTG
MunI 913 CAATTG C^AATTG
NheI 2652 GCTAGC G^CTAGC
NotI 208 GCGGCCGC GC^GGCCGC
PinAI 1050 ACCGGT A^CCGGT
SpeI 5702 ACTAGT A^CTAGT
SphI 3649 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 3977 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6004 GACCTCAGGTC
ApaI 949 GGGCCC GGGCC^C
4627 GGGCCC
AspEI 3977 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6004 GACCTCAGGTC
BamHI 243 GGATCC G^GATCC
3290 GGATCC
BbvCI 1285 CCTCAGC CCTCAGC(-5/-2)
81 GCTGAGG
BsaAI 975 TACGTG [CT]AC^GT[GA]
3824 CACGTA
BsaWI 1050 ACCGGT [AT]^CCGG[AT]
4568 TCCGGT
BspHI 3169 TCATGA T^CATGA
4422 TCATGA
BspMI 1769 ACCTGC ACCTGC(4/8)
879 GCAGGT
Eam1105I 3977 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6004 GACCTCAGGTC
EcoNI 4093 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4693 CCTAACTCAGG
HaeII 1860 GGCGCT [GA]GCGC^[CT]
3533 GGCGCT
KspI 48 CCGCGG CCGC^GG
215 CCGCGG
PflMI 3639 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5074 CCAAGTTCTGG
PshAI 1305 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5606 GACTTGGGTC
RcaI 3169 TCATGA T^CATGA
4422 TCATGA
SacII 48 CCGCGG CCGC^GG
215 CCGCGG
ScaI 4323 AGTACT AGT^ACT
4726 AGTACT
SexAI 1527 ACCTGGT A^CC[AT]GGT
3330 ACCTGGT
SspI 2720 AATATT AAT^ATT
4275 AATATT
StuI 3548 AGGCCT AGG^CCT
6073 AGGCCT
Van91I 3639 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5074 CCAAGTTCTGG
XbaI 3660 TCTAGA T^CTAGA
5414 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PaeR7I PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoI
XhoII