################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI BbrPI BciVI BsaAI BsaWI BseAI BspDI BspEI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI HpaI KpnI KspI MroI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspI SwaI XbaI XcmI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1893 GTCTAC GT^[AC][GT]AC AseI 4331 ATTAAT AT^TAAT AsnI 4331 ATTAAT AT^TAAT AvrII 4786 CCTAGG C^CTAGG BamHI 2990 GGATCC G^GATCC BbeI 272 GGCGCC GGCGC^C BbvCI 4638 CCTCAGC CCTCAGC(-5/-2) BclI 2079 TGATCA T^GATCA BglI 3796 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4786 CCTAGG C^CTAGG BlpI 246 GCTGAGC GC^T[ACGT]AGC Bpu1102I 246 GCTGAGC GC^T[ACGT]AGC BsiEI 639 CGACCG CG[GA][CT]^CG BsiWI 1343 CGTACG C^GTACG BsmBI 1117 GAGACG CGTCTC(1/5) BsmI 3501 GAATGC GAATGC(1/-1) BsrDI 2486 CATTGC GCAATG(2/0) CelII 246 GCTGAGC GC^T[ACGT]AGC DraIII 1490 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 676 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 2079 TGATCA T^GATCA HindIII 3955 AAGCTT A^AGCTT KasI 272 GGCGCC G^GCGCC MfeI 3331 CAATTG C^AATTG MluI 1716 ACGCGT A^CGCGT MunI 3331 CAATTG C^AATTG NarI 272 GGCGCC GG^CGCC NcoI 2877 CCATGG C^CATGG PaeR7I 3822 CTCGAG C^TCGAG PshBI 4331 ATTAAT AT^TAAT SapI 700 GAAGAGC GCTCTTC(1/4) ScaI 861 AGTACT AGT^ACT SfoI 272 GGCGCC GGC^GCC SmaI 4644 CCCGGG CCC^GGG SpeI 1257 ACTAGT A^CTAGT XhoI 3822 CTCGAG C^TCGAG XmaCI 4644 CCCGGG C^CCGGG XmaI 4644 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 272 GGCGCC G[GA]^CG[CT]C 478 GGCGTC AflII 1578 CTTAAG C^TTAAG 2308 CTTAAG Aor51HI 250 AGCGCT AGC^GCT 2291 AGCGCT Asp700I 1677 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3165 GAAGCCGTTC AviII 789 TGCGCA TGC^GCA 2414 TGCGCA BbsI 1521 GAAGAC GAAGAC(2/6) 1645 GTCTTC BfrI 1578 CTTAAG C^TTAAG 2308 CTTAAG BpuAI 1521 GAAGAC GAAGAC(2/6) 1645 GTCTTC BsaBI 889 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1404 GATCTTTATC BsaHI 272 GGCGCC G[GA]^CG[CT]C 478 GGCGTC Bsp1407I 2535 TGTACA T^GTACA 3927 TGTACA BspHI 2666 TCATGA T^CATGA 2759 TCATGA BspLU11I 3134 ACATGT A^CATGT 4223 ACATGT BspMI 1108 ACCTGC ACCTGC(4/8) 3221 ACCTGC BsrGI 2535 TGTACA T^GTACA 3927 TGTACA BstEII 947 GGTCACC G^GT[ACGT]ACC 4616 GGTTACC BstPI 947 GGTCACC G^GT[ACGT]ACC 4616 GGTTACC DrdI 757 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4046 GACAAGTGGGTC Eco47III 250 AGCGCT AGC^GCT 2291 AGCGCT EcoO65I 947 GGTCACC G^GT[ACGT]ACC 4616 GGTTACC EcoRI 2457 GAATTC G^AATTC 4844 GAATTC FspI 789 TGCGCA TGC^GCA 2414 TGCGCA HgaI 2341 GACGC GACGC(5/10) 479 GCGTC Hin1I 272 GGCGCC G[GA]^CG[CT]C 478 GGCGTC HincII 873 GTCAAC GT[CT]^[GA]AC 1966 GTTGAC HindII 873 GTCAAC GT[CT]^[GA]AC 1966 GTTGAC MaeII 2441 ACGT A^CGT 4079 ACGT MamI 889 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1404 GATCTTTATC PmeI 795 GTTTAAAC GTTT^AAAC 3459 GTTTAAAC PshAI 2965 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3944 GACAACAGTC RcaI 2666 TCATGA T^CATGA 2759 TCATGA SacI 2068 GAGCTC GAGCT^C 2203 GAGCTC SphI 2136 GCATGC GCATG^C 3683 GCATGC SspBI 2535 TGTACA T^GTACA 3927 TGTACA XmnI 1677 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3165 GAAGCCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII