##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI BbrPI BciVI BsaAI
BsaWI BseAI BspDI BspEI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI HpaI
KpnI KspI MroI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SspI SwaI XbaI
XcmI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1893 GTCTAC GT^[AC][GT]AC
AseI 4331 ATTAAT AT^TAAT
AsnI 4331 ATTAAT AT^TAAT
AvrII 4786 CCTAGG C^CTAGG
BamHI 2990 GGATCC G^GATCC
BbeI 272 GGCGCC GGCGC^C
BbvCI 4638 CCTCAGC CCTCAGC(-5/-2)
BclI 2079 TGATCA T^GATCA
BglI 3796 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4786 CCTAGG C^CTAGG
BlpI 246 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 246 GCTGAGC GC^T[ACGT]AGC
BsiEI 639 CGACCG CG[GA][CT]^CG
BsiWI 1343 CGTACG C^GTACG
BsmBI 1117 GAGACG CGTCTC(1/5)
BsmI 3501 GAATGC GAATGC(1/-1)
BsrDI 2486 CATTGC GCAATG(2/0)
CelII 246 GCTGAGC GC^T[ACGT]AGC
DraIII 1490 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 676 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2079 TGATCA T^GATCA
HindIII 3955 AAGCTT A^AGCTT
KasI 272 GGCGCC G^GCGCC
MfeI 3331 CAATTG C^AATTG
MluI 1716 ACGCGT A^CGCGT
MunI 3331 CAATTG C^AATTG
NarI 272 GGCGCC GG^CGCC
NcoI 2877 CCATGG C^CATGG
PaeR7I 3822 CTCGAG C^TCGAG
PshBI 4331 ATTAAT AT^TAAT
SapI 700 GAAGAGC GCTCTTC(1/4)
ScaI 861 AGTACT AGT^ACT
SfoI 272 GGCGCC GGC^GCC
SmaI 4644 CCCGGG CCC^GGG
SpeI 1257 ACTAGT A^CTAGT
XhoI 3822 CTCGAG C^TCGAG
XmaCI 4644 CCCGGG C^CCGGG
XmaI 4644 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 272 GGCGCC G[GA]^CG[CT]C
478 GGCGTC
AflII 1578 CTTAAG C^TTAAG
2308 CTTAAG
Aor51HI 250 AGCGCT AGC^GCT
2291 AGCGCT
Asp700I 1677 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3165 GAAGCCGTTC
AviII 789 TGCGCA TGC^GCA
2414 TGCGCA
BbsI 1521 GAAGAC GAAGAC(2/6)
1645 GTCTTC
BfrI 1578 CTTAAG C^TTAAG
2308 CTTAAG
BpuAI 1521 GAAGAC GAAGAC(2/6)
1645 GTCTTC
BsaBI 889 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1404 GATCTTTATC
BsaHI 272 GGCGCC G[GA]^CG[CT]C
478 GGCGTC
Bsp1407I 2535 TGTACA T^GTACA
3927 TGTACA
BspHI 2666 TCATGA T^CATGA
2759 TCATGA
BspLU11I 3134 ACATGT A^CATGT
4223 ACATGT
BspMI 1108 ACCTGC ACCTGC(4/8)
3221 ACCTGC
BsrGI 2535 TGTACA T^GTACA
3927 TGTACA
BstEII 947 GGTCACC G^GT[ACGT]ACC
4616 GGTTACC
BstPI 947 GGTCACC G^GT[ACGT]ACC
4616 GGTTACC
DrdI 757 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4046 GACAAGTGGGTC
Eco47III 250 AGCGCT AGC^GCT
2291 AGCGCT
EcoO65I 947 GGTCACC G^GT[ACGT]ACC
4616 GGTTACC
EcoRI 2457 GAATTC G^AATTC
4844 GAATTC
FspI 789 TGCGCA TGC^GCA
2414 TGCGCA
HgaI 2341 GACGC GACGC(5/10)
479 GCGTC
Hin1I 272 GGCGCC G[GA]^CG[CT]C
478 GGCGTC
HincII 873 GTCAAC GT[CT]^[GA]AC
1966 GTTGAC
HindII 873 GTCAAC GT[CT]^[GA]AC
1966 GTTGAC
MaeII 2441 ACGT A^CGT
4079 ACGT
MamI 889 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1404 GATCTTTATC
PmeI 795 GTTTAAAC GTTT^AAAC
3459 GTTTAAAC
PshAI 2965 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3944 GACAACAGTC
RcaI 2666 TCATGA T^CATGA
2759 TCATGA
SacI 2068 GAGCTC GAGCT^C
2203 GAGCTC
SphI 2136 GCATGC GCATG^C
3683 GCATGC
SspBI 2535 TGTACA T^GTACA
3927 TGTACA
XmnI 1677 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3165 GAAGCCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvI BcgI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII