##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AhdI AscI
AseI AsnI AspEI AspI AvrII
BbrPI BlnI BsaAI BseAI BsiWI
BspDI BspEI BstBI ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRV FseI HindIII HpaI MluI
MroI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SalI SbfI SfiI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 1858 GAATTC [GA]^AATT[CT]
AgeI 4595 ACCGGT A^CCGGT
ApoI 1858 GAATTC [GA]^AATT[CT]
AviII 5684 TGCGCA TGC^GCA
BbeI 4139 GGCGCC GGCGC^C
BciVI 235 GTATCC GTATCC(6/5)
BglII 4356 AGATCT A^GATCT
BsaBI 5065 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4595 ACCGGT [AT]^CCGG[AT]
BsiEI 5793 CGATCG CG[GA][CT]^CG
Bsp1407I 4051 TGTACA T^GTACA
BspHI 4764 TCATGA T^CATGA
BsrGI 4051 TGTACA T^GTACA
BssHII 154 GCGCGC G^CGCGC
Bst1107I 781 GTATAC GTA^TAC
BstXI 5898 CCAGGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 781 GTATAC GTA^TAC
EcoRI 1858 GAATTC G^AATTC
FspI 5684 TGCGCA TGC^GCA
KasI 4139 GGCGCC G^GCGCC
KspI 130 CCGCGG CCGC^GG
MamI 5065 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6369 CAATTG C^AATTG
MunI 6369 CAATTG C^AATTG
NarI 4139 GGCGCC GG^CGCC
NdeI 1958 CATATG CA^TATG
PinAI 4595 ACCGGT A^CCGGT
PvuI 5793 CGATCG CGAT^CG
RcaI 4764 TCATGA T^CATGA
SacII 130 CCGCGG CCGC^GG
SfoI 4139 GGCGCC GGC^GCC
SgrAI 157 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 4051 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 781 GTATAC GT^[AC][GT]AC
983 GTAGAC
AflII 3496 CTTAAG C^TTAAG
4345 CTTAAG
Aor51HI 794 AGCGCT AGC^GCT
1583 AGCGCT
Asp700I 1668 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3940 GAACCTCTTC
BamHI 3258 GGATCC G^GATCC
5444 GGATCC
BbsI 2995 GAAGAC GAAGAC(2/6)
5172 GTCTTC
BfrI 3496 CTTAAG C^TTAAG
4345 CTTAAG
BglI 4793 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6286 GCCGTGAGGGC
BlpI 1510 GCTCAGC GC^T[ACGT]AGC
2574 GCTGAGC
Bpu1102I 1510 GCTCAGC GC^T[ACGT]AGC
2574 GCTGAGC
BpuAI 2995 GAAGAC GAAGAC(2/6)
5172 GTCTTC
BsmBI 199 CGTCTC CGTCTC(1/5)
3334 GAGACG
BsrBI 5981 CCGCTC CCGCTC(-3/-3)
33 GAGCGG
BstEII 2170 GGTCACC G^GT[ACGT]ACC
4923 GGTCACC
BstPI 2170 GGTCACC G^GT[ACGT]ACC
4923 GGTCACC
CelII 1510 GCTCAGC GC^T[ACGT]AGC
2574 GCTGAGC
DrdI 191 GACTCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
330 GACTTGGCTGTC
Eco47III 794 AGCGCT AGC^GCT
1583 AGCGCT
EcoO65I 2170 GGTCACC G^GT[ACGT]ACC
4923 GGTCACC
EcoT22I 272 ATGCAT ATGCA^T
2257 ATGCAT
MaeII 1259 ACGT A^CGT
2246 ACGT
NsiI 272 ATGCAT ATGCA^T
2257 ATGCAT
ScaI 5570 AGTACT AGT^ACT
6221 AGTACT
SexAI 2882 ACCAGGT A^CC[AT]GGT
3288 ACCAGGT
XmnI 1668 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3940 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BclI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI