################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AccIII AclI AhdI AscI AseI AsnI AspEI AspI AvrII BbrPI BlnI BsaAI BseAI BsiWI BspDI BspEI BstBI ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV FseI HindIII HpaI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SalI SbfI SfiI SfuI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 1858 GAATTC [GA]^AATT[CT] AgeI 4595 ACCGGT A^CCGGT ApoI 1858 GAATTC [GA]^AATT[CT] AviII 5684 TGCGCA TGC^GCA BbeI 4139 GGCGCC GGCGC^C BciVI 235 GTATCC GTATCC(6/5) BglII 4356 AGATCT A^GATCT BsaBI 5065 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4595 ACCGGT [AT]^CCGG[AT] BsiEI 5793 CGATCG CG[GA][CT]^CG Bsp1407I 4051 TGTACA T^GTACA BspHI 4764 TCATGA T^CATGA BsrGI 4051 TGTACA T^GTACA BssHII 154 GCGCGC G^CGCGC Bst1107I 781 GTATAC GTA^TAC BstXI 5898 CCAGGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 781 GTATAC GTA^TAC EcoRI 1858 GAATTC G^AATTC FspI 5684 TGCGCA TGC^GCA KasI 4139 GGCGCC G^GCGCC KspI 130 CCGCGG CCGC^GG MamI 5065 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 6369 CAATTG C^AATTG MunI 6369 CAATTG C^AATTG NarI 4139 GGCGCC GG^CGCC NdeI 1958 CATATG CA^TATG PinAI 4595 ACCGGT A^CCGGT PvuI 5793 CGATCG CGAT^CG RcaI 4764 TCATGA T^CATGA SacII 130 CCGCGG CCGC^GG SfoI 4139 GGCGCC GGC^GCC SgrAI 157 CGCCGGTG C[GA]^CCGG[CT]G SspBI 4051 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 781 GTATAC GT^[AC][GT]AC 983 GTAGAC AflII 3496 CTTAAG C^TTAAG 4345 CTTAAG Aor51HI 794 AGCGCT AGC^GCT 1583 AGCGCT Asp700I 1668 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3940 GAACCTCTTC BamHI 3258 GGATCC G^GATCC 5444 GGATCC BbsI 2995 GAAGAC GAAGAC(2/6) 5172 GTCTTC BfrI 3496 CTTAAG C^TTAAG 4345 CTTAAG BglI 4793 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6286 GCCGTGAGGGC BlpI 1510 GCTCAGC GC^T[ACGT]AGC 2574 GCTGAGC Bpu1102I 1510 GCTCAGC GC^T[ACGT]AGC 2574 GCTGAGC BpuAI 2995 GAAGAC GAAGAC(2/6) 5172 GTCTTC BsmBI 199 CGTCTC CGTCTC(1/5) 3334 GAGACG BsrBI 5981 CCGCTC CCGCTC(-3/-3) 33 GAGCGG BstEII 2170 GGTCACC G^GT[ACGT]ACC 4923 GGTCACC BstPI 2170 GGTCACC G^GT[ACGT]ACC 4923 GGTCACC CelII 1510 GCTCAGC GC^T[ACGT]AGC 2574 GCTGAGC DrdI 191 GACTCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 330 GACTTGGCTGTC Eco47III 794 AGCGCT AGC^GCT 1583 AGCGCT EcoO65I 2170 GGTCACC G^GT[ACGT]ACC 4923 GGTCACC EcoT22I 272 ATGCAT ATGCA^T 2257 ATGCAT MaeII 1259 ACGT A^CGT 2246 ACGT NsiI 272 ATGCAT ATGCA^T 2257 ATGCAT ScaI 5570 AGTACT AGT^ACT 6221 AGTACT SexAI 2882 ACCAGGT A^CC[AT]GGT 3288 ACCAGGT XmnI 1668 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3940 GAACCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcgI BclI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI