################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AhdI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbeI BbrPI BcgI BfrI BglII BsaAI BsaHI BsiWI BsrBI BssHII BssSI BstEII BstPI CpoI DrdI Eam1105I EcoO65I EcoRV FspI Hin1I HpaI KasI KpnI KspI MfeI MluI MunI NarI NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 2418 GTATAC GT^[AC][GT]AC AccII 13 CGCG CG^CG AccIII 1544 TCCGGA T^CCGGA AflIII 2737 ACATGT A^C[GA][CT]GT AgeI 1820 ACCGGT A^CCGGT Alw44I 3538 GTGCAC G^TGCAC AocI 2775 CCTCAGG CC^T[ACGT]AGG ApaLI 3538 GTGCAC G^TGCAC AvrII 3783 CCTAGG C^CTAGG BanI 2717 GGCACC G^G[CT][GA]CC BanII 4472 GAGCCC G[GA]GC[CT]^C BclI 333 TGATCA T^GATCA BlnI 3783 CCTAGG C^CTAGG BsaBI 4627 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1544 TCCGGA T^CCGGA BsiEI 2032 CGGCCG CG[GA][CT]^CG BsmBI 2320 CGTCTC CGTCTC(1/5) BspDI 4025 ATCGAT AT^CGAT BspEI 1544 TCCGGA T^CCGGA BspHI 3453 TCATGA T^CATGA BspLU11I 2737 ACATGT A^CATGT Bst1107I 2418 GTATAC GTA^TAC BstBI 878 TTCGAA TT^CGAA BstUI 13 CGCG CG^CG BstXI 3419 CCAAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2418 GTATAC GTA^TAC Bsu36I 2775 CCTCAGG CC^T[ACGT]AGG ClaI 4025 ATCGAT AT^CGAT DraIII 4414 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2032 CGGCCG C^GGCCG EclXI 2032 CGGCCG C^GGCCG Eco52I 2032 CGGCCG C^GGCCG Eco81I 2775 CCTCAGG CC^T[ACGT]AGG EcoNI 1221 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3140 GAATTC G^AATTC EcoT22I 971 ATGCAT ATGCA^T FauI 1698 GCGGG CCCGC(4/6) FbaI 333 TGATCA T^GATCA FseI 2033 GGCCGGCC GGCCGG^CC HgaI 1624 GACGC GACGC(5/10) MaeII 4409 ACGT A^CGT MamI 4627 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1544 TCCGGA T^CCGGA MvnI 13 CGCG CG^CG NdeI 1887 CATATG CA^TATG NheI 2311 GCTAGC G^CTAGC NsiI 971 ATGCAT ATGCA^T NspV 878 TTCGAA TT^CGAA PinAI 1820 ACCGGT A^CCGGT RcaI 3453 TCATGA T^CATGA SexAI 3832 ACCAGGT A^CC[AT]GGT SfuI 878 TTCGAA TT^CGAA SgrAI 1384 CGCCGGCG C[GA]^CCGG[CT]G StuI 788 AGGCCT AGG^CCT XbaI 1023 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Aor51HI 818 AGCGCT AGC^GCT 1596 AGCGCT AspHI 250 GTGCTC G[AT]GC[AT]^C 3538 GTGCAC AvaI 1120 CCCGAG C^[CT]CG[GA]G 4563 CCCGAG BalI 773 TGGCCA TGG^CCA 1535 TGGCCA BbvCI 2753 CCTCAGC CCTCAGC(-5/-2) 2820 GCTGAGG BciVI 2257 GGATAC GTATCC(6/5) 1982 GTATCC BglI 1528 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3813 GCCCTCATGGC BlpI 925 GCTGAGC GC^T[ACGT]AGC 1966 GCTCAGC Bpu1102I 925 GCTGAGC GC^T[ACGT]AGC 1966 GCTCAGC BsaWI 1544 TCCGGA [AT]^CCGG[AT] 1820 ACCGGT BsiHKAI 250 GTGCTC G[AT]GC[AT]^C 3538 GTGCAC BsoBI 1120 CCCGAG C^[CT]CG[GA]G 4563 CCCGAG BsrDI 1632 CATTGC GCAATG(2/0) 2861 GCAATG CelII 925 GCTGAGC GC^T[ACGT]AGC 1966 GCTCAGC DsaI 2039 CCATGG C^C[GA][CT]GG 2423 CCATGG Eco47III 818 AGCGCT AGC^GCT 1596 AGCGCT MluNI 773 TGGCCA TGG^CCA 1535 TGGCCA MscI 773 TGGCCA TGG^CCA 1535 TGGCCA NaeI 1385 GCCGGC GCC^GGC 2034 GCCGGC NcoI 2039 CCATGG C^CATGG 2423 CCATGG NgoMIV 1385 GCCGGC G^CCGGC 2034 GCCGGC SapI 3000 GAAGAGC GCTCTTC(1/4) 2781 GCTCTTC XcmI 1460 CCAGGCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1818 CCACCGGTCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI