##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BcgI BfrI BglII
BsaAI BsaHI BsiWI BsrBI BssHII
BssSI BstEII BstPI CpoI DrdI
Eam1105I EcoO65I EcoRV FspI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NotI NruI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 2418 GTATAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AccIII 1544 TCCGGA T^CCGGA
AflIII 2737 ACATGT A^C[GA][CT]GT
AgeI 1820 ACCGGT A^CCGGT
Alw44I 3538 GTGCAC G^TGCAC
AocI 2775 CCTCAGG CC^T[ACGT]AGG
ApaLI 3538 GTGCAC G^TGCAC
AvrII 3783 CCTAGG C^CTAGG
BanI 2717 GGCACC G^G[CT][GA]CC
BanII 4472 GAGCCC G[GA]GC[CT]^C
BclI 333 TGATCA T^GATCA
BlnI 3783 CCTAGG C^CTAGG
BsaBI 4627 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1544 TCCGGA T^CCGGA
BsiEI 2032 CGGCCG CG[GA][CT]^CG
BsmBI 2320 CGTCTC CGTCTC(1/5)
BspDI 4025 ATCGAT AT^CGAT
BspEI 1544 TCCGGA T^CCGGA
BspHI 3453 TCATGA T^CATGA
BspLU11I 2737 ACATGT A^CATGT
Bst1107I 2418 GTATAC GTA^TAC
BstBI 878 TTCGAA TT^CGAA
BstUI 13 CGCG CG^CG
BstXI 3419 CCAAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2418 GTATAC GTA^TAC
Bsu36I 2775 CCTCAGG CC^T[ACGT]AGG
ClaI 4025 ATCGAT AT^CGAT
DraIII 4414 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2032 CGGCCG C^GGCCG
EclXI 2032 CGGCCG C^GGCCG
Eco52I 2032 CGGCCG C^GGCCG
Eco81I 2775 CCTCAGG CC^T[ACGT]AGG
EcoNI 1221 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3140 GAATTC G^AATTC
EcoT22I 971 ATGCAT ATGCA^T
FauI 1698 GCGGG CCCGC(4/6)
FbaI 333 TGATCA T^GATCA
FseI 2033 GGCCGGCC GGCCGG^CC
HgaI 1624 GACGC GACGC(5/10)
MaeII 4409 ACGT A^CGT
MamI 4627 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1544 TCCGGA T^CCGGA
MvnI 13 CGCG CG^CG
NdeI 1887 CATATG CA^TATG
NheI 2311 GCTAGC G^CTAGC
NsiI 971 ATGCAT ATGCA^T
NspV 878 TTCGAA TT^CGAA
PinAI 1820 ACCGGT A^CCGGT
RcaI 3453 TCATGA T^CATGA
SexAI 3832 ACCAGGT A^CC[AT]GGT
SfuI 878 TTCGAA TT^CGAA
SgrAI 1384 CGCCGGCG C[GA]^CCGG[CT]G
StuI 788 AGGCCT AGG^CCT
XbaI 1023 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Aor51HI 818 AGCGCT AGC^GCT
1596 AGCGCT
AspHI 250 GTGCTC G[AT]GC[AT]^C
3538 GTGCAC
AvaI 1120 CCCGAG C^[CT]CG[GA]G
4563 CCCGAG
BalI 773 TGGCCA TGG^CCA
1535 TGGCCA
BbvCI 2753 CCTCAGC CCTCAGC(-5/-2)
2820 GCTGAGG
BciVI 2257 GGATAC GTATCC(6/5)
1982 GTATCC
BglI 1528 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3813 GCCCTCATGGC
BlpI 925 GCTGAGC GC^T[ACGT]AGC
1966 GCTCAGC
Bpu1102I 925 GCTGAGC GC^T[ACGT]AGC
1966 GCTCAGC
BsaWI 1544 TCCGGA [AT]^CCGG[AT]
1820 ACCGGT
BsiHKAI 250 GTGCTC G[AT]GC[AT]^C
3538 GTGCAC
BsoBI 1120 CCCGAG C^[CT]CG[GA]G
4563 CCCGAG
BsrDI 1632 CATTGC GCAATG(2/0)
2861 GCAATG
CelII 925 GCTGAGC GC^T[ACGT]AGC
1966 GCTCAGC
DsaI 2039 CCATGG C^C[GA][CT]GG
2423 CCATGG
Eco47III 818 AGCGCT AGC^GCT
1596 AGCGCT
MluNI 773 TGGCCA TGG^CCA
1535 TGGCCA
MscI 773 TGGCCA TGG^CCA
1535 TGGCCA
NaeI 1385 GCCGGC GCC^GGC
2034 GCCGGC
NcoI 2039 CCATGG C^CATGG
2423 CCATGG
NgoMIV 1385 GCCGGC G^CCGGC
2034 GCCGGC
SapI 3000 GAAGAGC GCTCTTC(1/4)
2781 GCTCTTC
XcmI 1460 CCAGGCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1818 CCACCGGTCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI