##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII BalI BbrPI BcnI BglI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
Bsu36I CelII Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI FauI FseI FspI
HapII HpaII KpnI KspI MluI
MluNI MroI MscI MspI NaeI
NciI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 2490 CGCG CG^CG
AcyI 323 GGCGCC G[GA]^CG[CT]C
AflII 2508 CTTAAG C^TTAAG
Asp700I 3110 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2503 GAGCTC G[AT]GC[AT]^C
AspI 1986 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2615 GGATCC G^GATCC
BanI 323 GGCGCC G^G[CT][GA]CC
BanII 2503 GAGCTC G[GA]GC[CT]^C
BbeI 323 GGCGCC GGCGC^C
BbvCI 1923 CCTCAGC CCTCAGC(-5/-2)
BcgI 288 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 505 GTATCC GTATCC(6/5)
BclI 1625 TGATCA T^GATCA
BfrI 2508 CTTAAG C^TTAAG
BmyI 2503 GAGCTC G[AGT]GC[ACT]^C
BsaBI 1626 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 323 GGCGCC G[GA]^CG[CT]C
BsiHKAI 2503 GAGCTC G[AT]GC[AT]^C
Bsp1286I 2503 GAGCTC G[AGT]GC[ACT]^C
Bst1107I 4427 GTATAC GTA^TAC
BstEII 3012 GGTCACC G^GT[ACGT]ACC
BstPI 3012 GGTCACC G^GT[ACGT]ACC
BstUI 2490 CGCG CG^CG
BstZ17I 4427 GTATAC GTA^TAC
DraIII 1168 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 845 GACTGTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3385 CCATGG C^C[GA][CT]GG
EaeI 3159 TGGCCG [CT]^GGCC[GA]
EcoO65I 3012 GGTCACC G^GT[ACGT]ACC
EcoRV 1600 GATATC GAT^ATC
EcoT22I 690 ATGCAT ATGCA^T
FbaI 1625 TGATCA T^GATCA
HgaI 337 GACGC GACGC(5/10)
Hin1I 323 GGCGCC G[GA]^CG[CT]C
HpaI 3042 GTTAAC GTT^AAC
KasI 323 GGCGCC G^GCGCC
MamI 1626 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 830 CAGCTG C[AC]G^C[GT]G
MvnI 2490 CGCG CG^CG
NarI 323 GGCGCC GG^CGCC
NcoI 3385 CCATGG C^CATGG
NsiI 690 ATGCAT ATGCA^T
PflFI 1986 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 830 CAGCTG CAG^CTG
SacI 2503 GAGCTC GAGCT^C
SfoI 323 GGCGCC GGC^GCC
SnaBI 1693 TACGTA TAC^GTA
StuI 2695 AGGCCT AGG^CCT
Tth111I 1986 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4527 TCTAGA T^CTAGA
XcmI 766 CCATTTACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3110 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1914 GTAGAC GT^[AC][GT]AC
4427 GTATAC
AlwNI 1499 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3244 CAGGCACTG
AseI 2148 ATTAAT AT^TAAT
4581 ATTAAT
AsnI 2148 ATTAAT AT^TAAT
4581 ATTAAT
AvrII 178 CCTAGG C^CTAGG
5076 CCTAGG
BlnI 178 CCTAGG C^CTAGG
5076 CCTAGG
BpmI 2249 CTCCAG CTGGAG(16/14)
2500 CTGGAG
BsaAI 1693 TACGTA [CT]AC^GT[GA]
2908 TACGTG
BseRI 3465 CTCCTC GAGGAG(10/8)
528 GAGGAG
BsmI 643 GAATGC GAATGC(1/-1)
1829 GCATTC
BspLU11I 2775 ACATGT A^CATGT
4748 ACATGT
BsrDI 5039 CATTGC GCAATG(2/0)
5043 GCAATG
CfoI 269 GCGC GCG^C
324 GCGC
EcoRI 1661 GAATTC G^AATTC
2966 GAATTC
HaeII 268 GGCGCT [GA]GCGC^[CT]
323 GGCGCC
HhaI 269 GCGC GCG^C
324 GCGC
HinP1I 269 GCGC G^CGC
324 GCGC
MaeII 1694 ACGT A^CGT
2909 ACGT
MfeI 2586 CAATTG C^AATTG
2671 CAATTG
MunI 2586 CAATTG C^AATTG
2671 CAATTG
NheI 18 GCTAGC G^CTAGC
1614 GCTAGC
PleI 368 GAGTC GAGTC(4/5)
2282 GAGTC
PshBI 2148 ATTAAT AT^TAAT
4581 ATTAAT
SexAI 3008 ACCAGGT A^CC[AT]GGT
3218 ACCTGGT
SfcI 616 CTACAG C^T[GA][CT]AG
3569 CTGTAG
SmlI 2508 CTTAAG C^T[CT][GA]AG
3468 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbsI BbvI
BfaI BglII BpuAI BsaJI BsgI
BsiYI BslI BsmAI Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII