##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AscI AseI AsnI
AviII AvrII BlnI BsaBI BsiEI
BsiWI BspDI BspHI BsrDI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoT22I FseI FspI HpaI MamI
MluI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SmaI SpeI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AgeI 4413 ACCGGT A^CCGGT
Alw44I 1654 GTGCAC G^TGCAC
Aor51HI 601 AGCGCT AGC^GCT
ApaI 712 GGGCCC GGGCC^C
ApaLI 1654 GTGCAC G^TGCAC
AspI 3501 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1701 GGATCC G^GATCC
BbeI 86 GGCGCC GGCGC^C
BbvCI 4048 GCTGAGG CCTCAGC(-5/-2)
BglI 4277 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 555 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 555 GCTTAGC GC^T[ACGT]AGC
BsmI 3685 GAATGC GAATGC(1/-1)
Bsp1407I 1874 TGTACA T^GTACA
BsrGI 1874 TGTACA T^GTACA
BstAPI 2019 GCAGTCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 555 GCTTAGC GC^T[ACGT]AGC
DraIII 253 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4907 GACCACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2369 CCGCGG C^C[GA][CT]GG
Eco47III 601 AGCGCT AGC^GCT
EcoNI 633 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 436 GAATTC G^AATTC
EcoRV 4078 GATATC GAT^ATC
HindIII 4178 AAGCTT A^AGCTT
KasI 86 GGCGCC G^GCGCC
KspI 2369 CCGCGG CCGC^GG
NaeI 2777 GCCGGC GCC^GGC
NarI 86 GGCGCC GG^CGCC
NgoMIV 2777 GCCGGC G^CCGGC
PflFI 3501 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4413 ACCGGT A^CCGGT
PshAI 5 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2369 CCGCGG CCGC^GG
SapI 1608 GCTCTTC GCTCTTC(1/4)
SfoI 86 GGCGCC GGC^GCC
SgrAI 2776 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 2122 TACGTA TAC^GTA
SspBI 1874 TGTACA T^GTACA
SspI 5242 AATATT AAT^ATT
Tth111I 3501 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3081 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1931 GGTACC G^GTACC
3640 GGTACC
AccIII 31 TCCGGA T^CCGGA
401 TCCGGA
AflII 1615 CTTAAG C^TTAAG
4039 CTTAAG
Asp718I 1931 GGTACC G^GTACC
3640 GGTACC
AvaI 2980 CCCGAG C^[CT]CG[GA]G
3265 CCCGAG
BalI 1170 TGGCCA TGG^CCA
1424 TGGCCA
BbrPI 793 CACGTG CAC^GTG
3544 CACGTG
BcgI 2061 CGACTTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2279 CGAAGGAGCTGC
BciVI 2877 GGATAC GTATCC(6/5)
3780 GGATAC
BfrI 1615 CTTAAG C^TTAAG
4039 CTTAAG
BglII 237 AGATCT A^GATCT
5199 AGATCT
BsaI 3058 GAGACC GGTCTC(1/5)
4628 GAGACC
BseAI 31 TCCGGA T^CCGGA
401 TCCGGA
BsoBI 2980 CCCGAG C^[CT]CG[GA]G
3265 CCCGAG
BspEI 31 TCCGGA T^CCGGA
401 TCCGGA
BspLU11I 4004 ACATGT A^CATGT
4316 ACATGT
BsrBI 2178 CCGCTC CCGCTC(-3/-3)
4393 CCGCTC
BstEII 1267 GGTGACC G^GT[ACGT]ACC
1279 GGTGACC
BstPI 1267 GGTGACC G^GT[ACGT]ACC
1279 GGTGACC
DraI 3649 TTTAAA TTT^AAA
5247 TTTAAA
EcoO65I 1267 GGTGACC G^GT[ACGT]ACC
1279 GGTGACC
HincII 456 GTTGAC GT[CT]^[GA]AC
4885 GTTGAC
HindII 456 GTTGAC GT[CT]^[GA]AC
4885 GTTGAC
KpnI 1931 GGTACC GGTAC^C
3640 GGTACC
MfeI 1055 CAATTG C^AATTG
4949 CAATTG
MluNI 1170 TGGCCA TGG^CCA
1424 TGGCCA
MroI 31 TCCGGA T^CCGGA
401 TCCGGA
MscI 1170 TGGCCA TGG^CCA
1424 TGGCCA
MunI 1055 CAATTG C^AATTG
4949 CAATTG
PflMI 1430 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3447 CCAGCCAATGG
PmaCI 793 CACGTG CAC^GTG
3544 CACGTG
PmlI 793 CACGTG CAC^GTG
3544 CACGTG
SphI 2012 GCATGC GCATG^C
5147 GCATGC
Van91I 1430 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3447 CCAGCCAATGG
XcmI 1685 CCAAACACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3692 CCACTGTCTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApoI Asp700I AspEI
AspHI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI Eam1105I EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI