##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccIII AclI AflIII AgeI
AhdI Alw44I AocI ApaLI AscI
AspEI AspI AvaI BbeI BbrPI
BclI BsaAI BseAI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DrdI Eam1105I
Eco81I EcoRI EcoRV FbaI KasI
MluI MroI NarI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 941 GACGTC GACGT^C
Acc65I 3675 GGTACC G^GTACC
AccII 49 CGCG CG^CG
AcyI 941 GACGTC G[GA]^CG[CT]C
Aor51HI 1201 AGCGCT AGC^GCT
ApaI 2045 GGGCCC GGGCC^C
AseI 4261 ATTAAT AT^TAAT
AsnI 4261 ATTAAT AT^TAAT
Asp700I 4373 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3675 GGTACC G^GTACC
BamHI 1775 GGATCC G^GATCC
BcgI 946 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 721 CCCGG CC^[GC]GG
BglI 2880 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1396 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 941 GACGTC G[GA]^CG[CT]C
BsaWI 226 TCCGGT [AT]^CCGG[AT]
BspHI 201 TCATGA T^CATGA
BstEII 276 GGTCACC G^GT[ACGT]ACC
BstPI 276 GGTCACC G^GT[ACGT]ACC
BstUI 49 CGCG CG^CG
DraIII 822 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1201 AGCGCT AGC^GCT
EcoO65I 276 GGTCACC G^GT[ACGT]ACC
EcoT22I 4389 ATGCAT ATGCA^T
FauI 1536 CCCGC CCCGC(4/6)
FseI 42 GGCCGGCC GGCCGG^CC
Hin1I 941 GACGTC G[GA]^CG[CT]C
HindIII 3156 AAGCTT A^AGCTT
KpnI 3675 GGTACC GGTAC^C
KspI 48 CCGCGG CCGC^GG
MamI 1396 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2736 CAATTG C^AATTG
MunI 2736 CAATTG C^AATTG
MvnI 49 CGCG CG^CG
NaeI 43 GCCGGC GCC^GGC
NciI 721 CCCGG CC^[GC]GG
NcoI 1410 CCATGG C^CATGG
NgoMIV 43 GCCGGC G^CCGGC
NsiI 4389 ATGCAT ATGCA^T
PshBI 4261 ATTAAT AT^TAAT
PvuII 1684 CAGCTG CAG^CTG
RcaI 201 TCATGA T^CATGA
SacI 874 GAGCTC GAGCT^C
SacII 48 CCGCGG CCGC^GG
SapI 2199 GAAGAGC GCTCTTC(1/4)
SexAI 823 ACCAGGT A^CC[AT]GGT
SphI 3585 GCATGC GCATG^C
SspI 2462 AATATT AAT^ATT
XmnI 4373 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 2946 CTTAAG C^TTAAG
4640 CTTAAG
AlwNI 1066 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3608 CAGGAACTG
AviII 178 TGCGCA TGC^GCA
3573 TGCGCA
AvrII 1375 CCTAGG C^CTAGG
2251 CCTAGG
BanI 2034 GGTGCC G^G[CT][GA]CC
3675 GGTACC
BbsI 601 GAAGAC GAAGAC(2/6)
2691 GAAGAC
BfrI 2946 CTTAAG C^TTAAG
4640 CTTAAG
BlnI 1375 CCTAGG C^CTAGG
2251 CCTAGG
BpuAI 601 GAAGAC GAAGAC(2/6)
2691 GAAGAC
Bsp1407I 1966 TGTACA T^GTACA
2155 TGTACA
BspMI 949 ACCTGC ACCTGC(4/8)
149 GCAGGT
BsrGI 1966 TGTACA T^GTACA
2155 TGTACA
BstXI 1410 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4209 CCATGTGGCTGG
DsaI 48 CCGCGG C^C[GA][CT]GG
1410 CCATGG
EarI 257 CTCTTC CTCTTC(1/4)
2199 GAAGAG
FspI 178 TGCGCA TGC^GCA
3573 TGCGCA
HgaI 2060 GACGC GACGC(5/10)
2240 GCGTC
HpaI 328 GTTAAC GTT^AAC
3837 GTTAAC
Ksp632I 257 CTCTTC CTCTTC(1/4)
2199 GAAGAG
NheI 346 GCTAGC G^CTAGC
4604 GCTAGC
PflMI 821 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3481 CCAGAGTGTGG
PpuMI 1371 AGGTCCT [GA]G^G[AT]CC[CT]
4743 AGGACCC
ScaI 1761 AGTACT AGT^ACT
3071 AGTACT
SspBI 1966 TGTACA T^GTACA
2155 TGTACA
Van91I 821 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3481 CCAGAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanII
BbvCI BbvI BciVI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EagI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII