##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI BamHI BbeI
BbrPI BclI BfrI BsaBI BsaHI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrDI BssHII Bst1107I BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRV FbaI FseI
HaeII Hin1I HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SgrAI
SnaBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3233 GGTACC G^GTACC
AccII 601 CGCG CG^CG
AflIII 4410 ACGTGT A^C[GA][CT]GT
AocI 3755 CCTGAGG CC^T[ACGT]AGG
Asp718I 3233 GGTACC G^GTACC
BciVI 1612 GTATCC GTATCC(6/5)
BglII 1427 AGATCT A^GATCT
BlpI 1461 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1461 GCTCAGC GC^T[ACGT]AGC
BsaWI 309 ACCGGA [AT]^CCGG[AT]
BsiEI 635 CGGTCG CG[GA][CT]^CG
BsmI 268 GAATGC GAATGC(1/-1)
Bsp1407I 5066 TGTACA T^GTACA
BsrBI 856 CCGCTC CCGCTC(-3/-3)
BsrFI 1382 ACCGGC [GA]^CCGG[CT]
BsrGI 5066 TGTACA T^GTACA
BssSI 2231 CACGAG CACGAG(-5/-1)
BstAPI 263 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4081 TTCGAA TT^CGAA
BstUI 601 CGCG CG^CG
Bsu36I 3755 CCTGAGG CC^T[ACGT]AGG
CelII 1461 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1382 ACCGGC [GA]^CCGG[CT]
DraI 4256 TTTAAA TTT^AAA
Eco81I 3755 CCTGAGG CC^T[ACGT]AGG
EcoRI 227 GAATTC G^AATTC
EcoT22I 4054 ATGCAT ATGCA^T
HindIII 3947 AAGCTT A^AGCTT
KpnI 3233 GGTACC GGTAC^C
MvnI 601 CGCG CG^CG
NdeI 4441 CATATG CA^TATG
NheI 1121 GCTAGC G^CTAGC
NsiI 4054 ATGCAT ATGCA^T
NspV 4081 TTCGAA TT^CGAA
PshAI 1769 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2982 GCTCTTC GCTCTTC(1/4)
SbfI 3060 CCTGCAGG CCTGCA^GG
ScaI 2117 AGTACT AGT^ACT
SexAI 4465 ACCAGGT A^CC[AT]GGT
SfuI 4081 TTCGAA TT^CGAA
SpeI 2726 ACTAGT A^CTAGT
SphI 1757 GCATGC GCATG^C
Sse8387I 3060 CCTGCAGG CCTGCA^GG
SspBI 5066 TGTACA T^GTACA
XbaI 1743 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 106 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1443 GACTCTGAGTC
AspEI 106 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1443 GACTCTGAGTC
AviII 39 TGCGCA TGC^GCA
456 TGCGCA
AvrII 1664 CCTAGG C^CTAGG
3694 CCTAGG
BalI 1267 TGGCCA TGG^CCA
1312 TGGCCA
BcgI 1643 CGACACATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3424 CGATACTTCTGC
BlnI 1664 CCTAGG C^CTAGG
3694 CCTAGG
BsaAI 2716 TACGTG [CT]AC^GT[GA]
4409 TACGTG
BspHI 340 TCATGA T^CATGA
1038 TCATGA
BstEII 559 GGTCACC G^GT[ACGT]ACC
4291 GGTGACC
BstPI 559 GGTCACC G^GT[ACGT]ACC
4291 GGTGACC
Eam1105I 106 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1443 GACTCTGAGTC
EcoO65I 559 GGTCACC G^GT[ACGT]ACC
4291 GGTGACC
FspI 39 TGCGCA TGC^GCA
456 TGCGCA
HgaI 1718 GACGC GACGC(5/10)
4869 GCGTC
HincII 1395 GTCAAC GT[CT]^[GA]AC
2758 GTCAAC
HindII 1395 GTCAAC GT[CT]^[GA]AC
2758 GTCAAC
MluNI 1267 TGGCCA TGG^CCA
1312 TGGCCA
MscI 1267 TGGCCA TGG^CCA
1312 TGGCCA
NspI 1415 ACATGC [GA]CATG^[CT]
1757 GCATGC
PvuII 1287 CAGCTG CAG^CTG
4394 CAGCTG
RcaI 340 TCATGA T^CATGA
1038 TCATGA
SmaI 315 CCCGGG CCC^GGG
3966 CCCGGG
XmaCI 315 CCCGGG C^CCGGG
3966 CCCGGG
XmaI 315 CCCGGG C^CCGGG
3966 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI