################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI BamHI BbeI BbrPI BclI BfrI BsaBI BsaHI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI HaeII Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SgrAI SnaBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 3233 GGTACC G^GTACC AccII 601 CGCG CG^CG AflIII 4410 ACGTGT A^C[GA][CT]GT AocI 3755 CCTGAGG CC^T[ACGT]AGG Asp718I 3233 GGTACC G^GTACC BciVI 1612 GTATCC GTATCC(6/5) BglII 1427 AGATCT A^GATCT BlpI 1461 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1461 GCTCAGC GC^T[ACGT]AGC BsaWI 309 ACCGGA [AT]^CCGG[AT] BsiEI 635 CGGTCG CG[GA][CT]^CG BsmI 268 GAATGC GAATGC(1/-1) Bsp1407I 5066 TGTACA T^GTACA BsrBI 856 CCGCTC CCGCTC(-3/-3) BsrFI 1382 ACCGGC [GA]^CCGG[CT] BsrGI 5066 TGTACA T^GTACA BssSI 2231 CACGAG CACGAG(-5/-1) BstAPI 263 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4081 TTCGAA TT^CGAA BstUI 601 CGCG CG^CG Bsu36I 3755 CCTGAGG CC^T[ACGT]AGG CelII 1461 GCTCAGC GC^T[ACGT]AGC Cfr10I 1382 ACCGGC [GA]^CCGG[CT] DraI 4256 TTTAAA TTT^AAA Eco81I 3755 CCTGAGG CC^T[ACGT]AGG EcoRI 227 GAATTC G^AATTC EcoT22I 4054 ATGCAT ATGCA^T HindIII 3947 AAGCTT A^AGCTT KpnI 3233 GGTACC GGTAC^C MvnI 601 CGCG CG^CG NdeI 4441 CATATG CA^TATG NheI 1121 GCTAGC G^CTAGC NsiI 4054 ATGCAT ATGCA^T NspV 4081 TTCGAA TT^CGAA PshAI 1769 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2982 GCTCTTC GCTCTTC(1/4) SbfI 3060 CCTGCAGG CCTGCA^GG ScaI 2117 AGTACT AGT^ACT SexAI 4465 ACCAGGT A^CC[AT]GGT SfuI 4081 TTCGAA TT^CGAA SpeI 2726 ACTAGT A^CTAGT SphI 1757 GCATGC GCATG^C Sse8387I 3060 CCTGCAGG CCTGCA^GG SspBI 5066 TGTACA T^GTACA XbaI 1743 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AhdI 106 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1443 GACTCTGAGTC AspEI 106 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1443 GACTCTGAGTC AviII 39 TGCGCA TGC^GCA 456 TGCGCA AvrII 1664 CCTAGG C^CTAGG 3694 CCTAGG BalI 1267 TGGCCA TGG^CCA 1312 TGGCCA BcgI 1643 CGACACATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3424 CGATACTTCTGC BlnI 1664 CCTAGG C^CTAGG 3694 CCTAGG BsaAI 2716 TACGTG [CT]AC^GT[GA] 4409 TACGTG BspHI 340 TCATGA T^CATGA 1038 TCATGA BstEII 559 GGTCACC G^GT[ACGT]ACC 4291 GGTGACC BstPI 559 GGTCACC G^GT[ACGT]ACC 4291 GGTGACC Eam1105I 106 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1443 GACTCTGAGTC EcoO65I 559 GGTCACC G^GT[ACGT]ACC 4291 GGTGACC FspI 39 TGCGCA TGC^GCA 456 TGCGCA HgaI 1718 GACGC GACGC(5/10) 4869 GCGTC HincII 1395 GTCAAC GT[CT]^[GA]AC 2758 GTCAAC HindII 1395 GTCAAC GT[CT]^[GA]AC 2758 GTCAAC MluNI 1267 TGGCCA TGG^CCA 1312 TGGCCA MscI 1267 TGGCCA TGG^CCA 1312 TGGCCA NspI 1415 ACATGC [GA]CATG^[CT] 1757 GCATGC PvuII 1287 CAGCTG CAG^CTG 4394 CAGCTG RcaI 340 TCATGA T^CATGA 1038 TCATGA SmaI 315 CCCGGG CCC^GGG 3966 CCCGGG XmaCI 315 CCCGGG C^CCGGG 3966 CCCGGG XmaI 315 CCCGGG C^CCGGG 3966 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI