##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AgeI ApaI AscI AseI AsnI
Asp700I AviII BciVI BclI BsiWI
BspLU11I BssHII BssSI BstBI BstEII
BstPI CpoI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII KspI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SpeI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 4261 GACGTC GACGT^C
Acc65I 5240 GGTACC G^GTACC
AccII 2890 CGCG CG^CG
AccIII 2903 TCCGGA T^CCGGA
AclI 3200 AACGTT AA^CGTT
AocI 2080 CCTAAGG CC^T[ACGT]AGG
Asp718I 5240 GGTACC G^GTACC
AspI 237 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4216 CCCGAG C^[CT]CG[GA]G
AvrII 245 CCTAGG C^CTAGG
BamHI 2407 GGATCC G^GATCC
BbeI 2067 GGCGCC GGCGC^C
BbrPI 4777 CACGTG CAC^GTG
BcgI 2195 CGATTTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 245 CCTAGG C^CTAGG
BsaBI 4372 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2903 TCCGGA [AT]^CCGG[AT]
BseAI 2903 TCCGGA T^CCGGA
BsoBI 4216 CCCGAG C^[CT]CG[GA]G
BspDI 3311 ATCGAT AT^CGAT
BspEI 2903 TCCGGA T^CCGGA
BsrBI 3138 CCGCTC CCGCTC(-3/-3)
Bst1107I 614 GTATAC GTA^TAC
BstUI 2890 CGCG CG^CG
BstZ17I 614 GTATAC GTA^TAC
Bsu36I 2080 CCTAAGG CC^T[ACGT]AGG
ClaI 3311 ATCGAT AT^CGAT
Eco81I 2080 CCTAAGG CC^T[ACGT]AGG
HgaI 2888 GACGC GACGC(5/10)
HpaI 4386 GTTAAC GTT^AAC
KasI 2067 GGCGCC G^GCGCC
KpnI 5240 GGTACC GGTAC^C
MamI 4372 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2903 TCCGGA T^CCGGA
MvnI 2890 CGCG CG^CG
NarI 2067 GGCGCC GG^CGCC
NheI 1217 GCTAGC G^CTAGC
PflFI 237 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4777 CACGTG CAC^GTG
PmlI 4777 CACGTG CAC^GTG
Psp1406I 3200 AACGTT AA^CGTT
SfoI 2067 GGCGCC GGC^GCC
SnaBI 611 TACGTA TAC^GTA
SphI 1770 GCATGC GCATG^C
Tth111I 237 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 614 GTATAC GT^[AC][GT]AC
2643 GTAGAC
AcyI 2067 GGCGCC G[GA]^CG[CT]C
4261 GACGTC
AflII 1183 CTTAAG C^TTAAG
2487 CTTAAG
AflIII 1426 ACACGT A^C[GA][CT]GT
4778 ACGTGT
AhdI 1457 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2372 GACTGCAGGTC
Alw44I 1832 GTGCAC G^TGCAC
4774 GTGCAC
AlwNI 2532 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
4675 CAGCAGCTG
ApaLI 1832 GTGCAC G^TGCAC
4774 GTGCAC
AspEI 1457 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2372 GACTGCAGGTC
BalI 398 TGGCCA TGG^CCA
4237 TGGCCA
BbvCI 3185 CCTCAGC CCTCAGC(-5/-2)
4253 CCTCAGC
BfrI 1183 CTTAAG C^TTAAG
2487 CTTAAG
BglI 325 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4571 GCCGGGCAGGC
BglII 1050 AGATCT A^GATCT
1171 AGATCT
BlpI 743 GCTGAGC GC^T[ACGT]AGC
939 GCTGAGC
Bpu1102I 743 GCTGAGC GC^T[ACGT]AGC
939 GCTGAGC
BsaHI 2067 GGCGCC G[GA]^CG[CT]C
4261 GACGTC
BsiEI 2774 CGACCG CG[GA][CT]^CG
4141 CGACCG
BsmBI 3550 GAGACG CGTCTC(1/5)
4608 GAGACG
BsmI 1409 GAATGC GAATGC(1/-1)
1579 GAATGC
Bsp1407I 1085 TGTACA T^GTACA
5490 TGTACA
BspHI 3019 TCATGA T^CATGA
4722 TCATGA
BsrGI 1085 TGTACA T^GTACA
5490 TGTACA
CelII 743 GCTGAGC GC^T[ACGT]AGC
939 GCTGAGC
DraIII 681 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4768 CACGCCGTG
DrdI 3391 GACGGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4261 GACGTCATTGTC
Eam1105I 1457 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2372 GACTGCAGGTC
EcoNI 2459 CCTAAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2503 CCTGACACAGG
Hin1I 2067 GGCGCC G[GA]^CG[CT]C
4261 GACGTC
MluNI 398 TGGCCA TGG^CCA
4237 TGGCCA
MscI 398 TGGCCA TGG^CCA
4237 TGGCCA
NspI 1770 GCATGC [GA]CATG^[CT]
5348 GCATGT
PshAI 2360 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3982 GACAGCAGTC
RcaI 3019 TCATGA T^CATGA
4722 TCATGA
SacI 2957 GAGCTC GAGCT^C
4036 GAGCTC
SspBI 1085 TGTACA T^GTACA
5490 TGTACA
StuI 323 AGGCCT AGG^CCT
2511 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
Aor51HI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII