################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI BbeI BbrPI BclI BfrI BsaAI BsaBI BsaHI BsiWI BspDI BssHII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI Hin1I HpaI KasI KspI MamI MluI NarI NdeI NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI PshBI RsrII SacII SalI SbfI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 2207 GGTACC G^GTACC AccIII 407 TCCGGA T^CCGGA AclI 2408 AACGTT AA^CGTT AocI 1559 CCTGAGG CC^T[ACGT]AGG ApaI 2627 GGGCCC GGGCC^C Asp718I 2207 GGTACC G^GTACC AspHI 1200 GAGCTC G[AT]GC[AT]^C AviII 173 TGCGCA TGC^GCA AvrII 3632 CCTAGG C^CTAGG BalI 1752 TGGCCA TGG^CCA BamHI 2048 GGATCC G^GATCC BbvCI 3105 CCTCAGC CCTCAGC(-5/-2) BcgI 3875 GCATCTGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1439 GTATCC GTATCC(6/5) BglI 2586 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3126 AGATCT A^GATCT BlnI 3632 CCTAGG C^CTAGG BseAI 407 TCCGGA T^CCGGA BsiEI 151 CGATCG CG[GA][CT]^CG BsiHKAI 1200 GAGCTC G[AT]GC[AT]^C BsmBI 2172 GAGACG CGTCTC(1/5) BsmI 2599 GAATGC GAATGC(1/-1) BspEI 407 TCCGGA T^CCGGA BspHI 4220 TCATGA T^CATGA BsrBI 4468 CCGCTC CCGCTC(-3/-3) BsrDI 4520 CATTGC GCAATG(2/0) BsrFI 2955 GCCGGC [GA]^CCGG[CT] Bst1107I 2939 GTATAC GTA^TAC BstBI 1828 TTCGAA TT^CGAA BstZ17I 2939 GTATAC GTA^TAC Bsu36I 1559 CCTGAGG CC^T[ACGT]AGG Cfr10I 2955 GCCGGC [GA]^CCGG[CT] DraIII 1293 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1559 CCTGAGG CC^T[ACGT]AGG EcoT22I 4281 ATGCAT ATGCA^T FspI 173 TGCGCA TGC^GCA KpnI 2207 GGTACC GGTAC^C MfeI 3468 CAATTG C^AATTG MluNI 1752 TGGCCA TGG^CCA MroI 407 TCCGGA T^CCGGA MscI 1752 TGGCCA TGG^CCA MunI 3468 CAATTG C^AATTG NaeI 2955 GCCGGC GCC^GGC NgoMIV 2955 GCCGGC G^CCGGC NheI 236 GCTAGC G^CTAGC NsiI 4281 ATGCAT ATGCA^T NspV 1828 TTCGAA TT^CGAA PaeR7I 4087 CTCGAG C^TCGAG PpuMI 2245 GGGACCC [GA]G^G[AT]CC[CT] Psp1406I 2408 AACGTT AA^CGTT PvuI 151 CGATCG CGAT^CG RcaI 4220 TCATGA T^CATGA SacI 1200 GAGCTC GAGCT^C SfiI 2585 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1828 TTCGAA TT^CGAA SphI 1957 GCATGC GCATG^C SspI 4768 AATATT AAT^ATT XhoI 4087 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 636 GTAGAC GT^[AC][GT]AC 2939 GTATAC AccII 218 CGCG CG^CG 3683 CGCG AspI 1620 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3315 GACTTGGTC BanI 71 GGCACC G^G[CT][GA]CC 2207 GGTACC BlpI 4470 GCTCAGC GC^T[ACGT]AGC 4533 GCTGAGC Bpu1102I 4470 GCTCAGC GC^T[ACGT]AGC 4533 GCTGAGC BsaWI 407 TCCGGA [AT]^CCGG[AT] 2210 ACCGGA Bsp1407I 3808 TGTACA T^GTACA 4267 TGTACA BsrGI 3808 TGTACA T^GTACA 4267 TGTACA BssSI 582 CACGAG CACGAG(-5/-1) 956 CACGAG BstUI 218 CGCG CG^CG 3683 CGCG CelII 4470 GCTCAGC GC^T[ACGT]AGC 4533 GCTGAGC DraI 2330 TTTAAA TTT^AAA 3117 TTTAAA DraII 2245 GGGACCC [GA]G^G[ACGT]CC[CT] 2522 AGGCCCT EaeI 1043 CGGCCA [CT]^GGCC[GA] 1752 TGGCCA EcoO109I 2245 GGGACCC [GA]G^G[ACGT]CC[CT] 2522 AGGCCCT HincII 1589 GTCAAC GT[CT]^[GA]AC 3951 GTTGAC HindII 1589 GTCAAC GT[CT]^[GA]AC 3951 GTTGAC HindIII 105 AAGCTT A^AGCTT 3596 AAGCTT MvnI 218 CGCG CG^CG 3683 CGCG PflFI 1620 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3315 GACTTGGTC PvuII 2742 CAGCTG CAG^CTG 4531 CAGCTG SapI 2811 GAAGAGC GCTCTTC(1/4) 3800 GCTCTTC ScaI 1486 AGTACT AGT^ACT 4716 AGTACT SexAI 1294 ACCAGGT A^CC[AT]GGT 2633 ACCTGGT SspBI 3808 TGTACA T^GTACA 4267 TGTACA StuI 424 AGGCCT AGG^CCT 802 AGGCCT Tth111I 1620 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3315 GACTTGGTC XbaI 938 TCTAGA T^CTAGA 1370 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoNI EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI