##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI BbeI BbrPI BclI
BfrI BsaAI BsaBI BsaHI BsiWI
BspDI BssHII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI Hin1I
HpaI KasI KspI MamI MluI
NarI NdeI NotI NruI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI RsrII SacII SalI SbfI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 2207 GGTACC G^GTACC
AccIII 407 TCCGGA T^CCGGA
AclI 2408 AACGTT AA^CGTT
AocI 1559 CCTGAGG CC^T[ACGT]AGG
ApaI 2627 GGGCCC GGGCC^C
Asp718I 2207 GGTACC G^GTACC
AspHI 1200 GAGCTC G[AT]GC[AT]^C
AviII 173 TGCGCA TGC^GCA
AvrII 3632 CCTAGG C^CTAGG
BalI 1752 TGGCCA TGG^CCA
BamHI 2048 GGATCC G^GATCC
BbvCI 3105 CCTCAGC CCTCAGC(-5/-2)
BcgI 3875 GCATCTGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1439 GTATCC GTATCC(6/5)
BglI 2586 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3126 AGATCT A^GATCT
BlnI 3632 CCTAGG C^CTAGG
BseAI 407 TCCGGA T^CCGGA
BsiEI 151 CGATCG CG[GA][CT]^CG
BsiHKAI 1200 GAGCTC G[AT]GC[AT]^C
BsmBI 2172 GAGACG CGTCTC(1/5)
BsmI 2599 GAATGC GAATGC(1/-1)
BspEI 407 TCCGGA T^CCGGA
BspHI 4220 TCATGA T^CATGA
BsrBI 4468 CCGCTC CCGCTC(-3/-3)
BsrDI 4520 CATTGC GCAATG(2/0)
BsrFI 2955 GCCGGC [GA]^CCGG[CT]
Bst1107I 2939 GTATAC GTA^TAC
BstBI 1828 TTCGAA TT^CGAA
BstZ17I 2939 GTATAC GTA^TAC
Bsu36I 1559 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2955 GCCGGC [GA]^CCGG[CT]
DraIII 1293 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1559 CCTGAGG CC^T[ACGT]AGG
EcoT22I 4281 ATGCAT ATGCA^T
FspI 173 TGCGCA TGC^GCA
KpnI 2207 GGTACC GGTAC^C
MfeI 3468 CAATTG C^AATTG
MluNI 1752 TGGCCA TGG^CCA
MroI 407 TCCGGA T^CCGGA
MscI 1752 TGGCCA TGG^CCA
MunI 3468 CAATTG C^AATTG
NaeI 2955 GCCGGC GCC^GGC
NgoMIV 2955 GCCGGC G^CCGGC
NheI 236 GCTAGC G^CTAGC
NsiI 4281 ATGCAT ATGCA^T
NspV 1828 TTCGAA TT^CGAA
PaeR7I 4087 CTCGAG C^TCGAG
PpuMI 2245 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2408 AACGTT AA^CGTT
PvuI 151 CGATCG CGAT^CG
RcaI 4220 TCATGA T^CATGA
SacI 1200 GAGCTC GAGCT^C
SfiI 2585 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1828 TTCGAA TT^CGAA
SphI 1957 GCATGC GCATG^C
SspI 4768 AATATT AAT^ATT
XhoI 4087 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 636 GTAGAC GT^[AC][GT]AC
2939 GTATAC
AccII 218 CGCG CG^CG
3683 CGCG
AspI 1620 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3315 GACTTGGTC
BanI 71 GGCACC G^G[CT][GA]CC
2207 GGTACC
BlpI 4470 GCTCAGC GC^T[ACGT]AGC
4533 GCTGAGC
Bpu1102I 4470 GCTCAGC GC^T[ACGT]AGC
4533 GCTGAGC
BsaWI 407 TCCGGA [AT]^CCGG[AT]
2210 ACCGGA
Bsp1407I 3808 TGTACA T^GTACA
4267 TGTACA
BsrGI 3808 TGTACA T^GTACA
4267 TGTACA
BssSI 582 CACGAG CACGAG(-5/-1)
956 CACGAG
BstUI 218 CGCG CG^CG
3683 CGCG
CelII 4470 GCTCAGC GC^T[ACGT]AGC
4533 GCTGAGC
DraI 2330 TTTAAA TTT^AAA
3117 TTTAAA
DraII 2245 GGGACCC [GA]G^G[ACGT]CC[CT]
2522 AGGCCCT
EaeI 1043 CGGCCA [CT]^GGCC[GA]
1752 TGGCCA
EcoO109I 2245 GGGACCC [GA]G^G[ACGT]CC[CT]
2522 AGGCCCT
HincII 1589 GTCAAC GT[CT]^[GA]AC
3951 GTTGAC
HindII 1589 GTCAAC GT[CT]^[GA]AC
3951 GTTGAC
HindIII 105 AAGCTT A^AGCTT
3596 AAGCTT
MvnI 218 CGCG CG^CG
3683 CGCG
PflFI 1620 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3315 GACTTGGTC
PvuII 2742 CAGCTG CAG^CTG
4531 CAGCTG
SapI 2811 GAAGAGC GCTCTTC(1/4)
3800 GCTCTTC
ScaI 1486 AGTACT AGT^ACT
4716 AGTACT
SexAI 1294 ACCAGGT A^CC[AT]GGT
2633 ACCTGGT
SspBI 3808 TGTACA T^GTACA
4267 TGTACA
StuI 424 AGGCCT AGG^CCT
802 AGGCCT
Tth111I 1620 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3315 GACTTGGTC
XbaI 938 TCTAGA T^CTAGA
1370 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoNI EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI