##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AclI AgeI AocI AscI
Asp718I AviII BciVI BsiWI BspDI
BspHI BspLU11I Bst1107I BstZ17I Bsu36I
ClaI CpoI DraIII DrdI Eco81I
EcoRV FspI HincII HindII HindIII
HpaI KpnI MfeI MunI NdeI
NheI NotI NruI PacI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SalI SgrAI SmaI SnaBI SpeI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 5000 GACGTC GACGT^C
AccIII 4135 TCCGGA T^CCGGA
AlwNI 3445 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 5686 ATTAAT AT^TAAT
AsnI 5686 ATTAAT AT^TAAT
AvrII 3360 CCTAGG C^CTAGG
BamHI 3067 GGATCC G^GATCC
BcgI 1264 GCACCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3360 CCTAGG C^CTAGG
BsaBI 2459 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4135 TCCGGA T^CCGGA
BsmBI 4388 GAGACG CGTCTC(1/5)
BsmI 5567 GCATTC GAATGC(1/-1)
BspEI 4135 TCCGGA T^CCGGA
BssHII 4334 GCGCGC G^CGCGC
BstBI 2708 TTCGAA TT^CGAA
BstEII 1598 GGTTACC G^GT[ACGT]ACC
BstPI 1598 GGTTACC G^GT[ACGT]ACC
EcoNI 1106 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1598 GGTTACC G^GT[ACGT]ACC
EcoT22I 2067 ATGCAT ATGCA^T
FseI 4393 GGCCGGCC GGCCGG^CC
MamI 2459 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1315 ACGCGT A^CGCGT
MroI 4135 TCCGGA T^CCGGA
NsiI 2067 ATGCAT ATGCA^T
NspV 2708 TTCGAA TT^CGAA
PaeR7I 1272 CTCGAG C^TCGAG
PshBI 5686 ATTAAT AT^TAAT
SbfI 1382 CCTGCAGG CCTGCA^GG
ScaI 5904 AGTACT AGT^ACT
SexAI 565 ACCAGGT A^CC[AT]GGT
SfuI 2708 TTCGAA TT^CGAA
Sse8387I 1382 CCTGCAGG CCTGCA^GG
SwaI 5329 ATTTAAAT ATTT^AAAT
XhoI 1272 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 1644 CTTAAG C^TTAAG
5453 CTTAAG
AflIII 445 ACGTGT A^C[GA][CT]GT
1315 ACGCGT
Alw44I 54 GTGCAC G^TGCAC
4672 GTGCAC
ApaI 102 GGGCCC GGGCC^C
2046 GGGCCC
ApaLI 54 GTGCAC G^TGCAC
4672 GTGCAC
AspI 42 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3923 GACGCCGTC
BbeI 2106 GGCGCC GGCGC^C
3575 GGCGCC
BbrPI 1512 CACGTG CAC^GTG
2997 CACGTG
BbvCI 3796 CCTCAGC CCTCAGC(-5/-2)
2037 GCTGAGG
BclI 1858 TGATCA T^GATCA
4814 TGATCA
BfrI 1644 CTTAAG C^TTAAG
5453 CTTAAG
BglII 2254 AGATCT A^GATCT
3503 AGATCT
BsaAI 1512 CACGTG [CT]AC^GT[GA]
2997 CACGTG
BsiEI 2089 CGGCCG CG[GA][CT]^CG
4392 CGGCCG
BstXI 562 CCAACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2050 CCAGCACCATGG
EagI 2089 CGGCCG C^GGCCG
4392 CGGCCG
EclXI 2089 CGGCCG C^GGCCG
4392 CGGCCG
Eco52I 2089 CGGCCG C^GGCCG
4392 CGGCCG
EcoRI 4020 GAATTC G^AATTC
5821 GAATTC
FbaI 1858 TGATCA T^GATCA
4814 TGATCA
KasI 2106 GGCGCC G^GCGCC
3575 GGCGCC
NarI 2106 GGCGCC GG^CGCC
3575 GGCGCC
PflFI 42 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3923 GACGCCGTC
PmaCI 1512 CACGTG CAC^GTG
2997 CACGTG
PmlI 1512 CACGTG CAC^GTG
2997 CACGTG
PshAI 2316 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3199 GACCTCGGTC
SacI 593 GAGCTC GAGCT^C
1806 GAGCTC
SapI 909 GAAGAGC GCTCTTC(1/4)
3087 GAAGAGC
SfiI 2090 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2675 GGCCTGCTGGGCC
SfoI 2106 GGCGCC GGC^GCC
3575 GGCGCC
SphI 2116 GCATGC GCATG^C
2801 GCATGC
Tth111I 42 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3923 GACGCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI Aor51HI
ApoI Asp700I AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI