##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI BbrPI
BciVI BfrI BglII BseAI BsiEI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SbfI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2553 GACGTC GACGT^C
AflIII 669 ACATGT A^C[GA][CT]GT
Alw44I 3334 GTGCAC G^TGCAC
ApaI 4828 GGGCCC GGGCC^C
ApaLI 3334 GTGCAC G^TGCAC
AviII 6381 TGCGCA TGC^GCA
AvrII 4823 CCTAGG C^CTAGG
BbeI 2605 GGCGCC GGCGC^C
BlnI 4823 CCTAGG C^CTAGG
BsaAI 2523 TACGTG [CT]AC^GT[GA]
BsaBI 2574 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3473 TGTACA T^GTACA
BspHI 6422 TCATGA T^CATGA
BspLU11I 669 ACATGT A^CATGT
BsrDI 6468 GCAATG GCAATG(2/0)
BsrGI 3473 TGTACA T^GTACA
BssSI 1871 CACGAG CACGAG(-5/-1)
CpoI 2232 CGGACCG CG^G[AT]CCG
DrdI 2333 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 6381 TGCGCA TGC^GCA
HpaI 3563 GTTAAC GTT^AAC
KasI 2605 GGCGCC G^GCGCC
KspI 26 CCGCGG CCGC^GG
MamI 2574 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5898 GCCGGC GCC^GGC
NarI 2605 GGCGCC GG^CGCC
NdeI 835 CATATG CA^TATG
NgoMIV 5898 GCCGGC G^CCGGC
NheI 2645 GCTAGC G^CTAGC
PshAI 1116 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 6422 TCATGA T^CATGA
RsrII 2232 CGGACCG CG^G[AT]CCG
SacII 26 CCGCGG CCGC^GG
SalI 1670 GTCGAC G^TCGAC
SapI 233 GCTCTTC GCTCTTC(1/4)
ScaI 2361 AGTACT AGT^ACT
SexAI 3338 ACCAGGT A^CC[AT]GGT
SfoI 2605 GGCGCC GGC^GCC
SpeI 4455 ACTAGT A^CTAGT
SphI 3143 GCATGC GCATG^C
SspBI 3473 TGTACA T^GTACA
SspI 938 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 4947 GGTACC G^GTACC
6174 GGTACC
AccI 1670 GTCGAC GT^[AC][GT]AC
2933 GTCTAC
AccII 27 CGCG CG^CG
3011 CGCG
Aor51HI 193 AGCGCT AGC^GCT
2759 AGCGCT
Asp700I 1507 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4731 GAACCTGTTC
Asp718I 4947 GGTACC G^GTACC
6174 GGTACC
BamHI 2474 GGATCC G^GATCC
6018 GGATCC
BclI 1082 TGATCA T^GATCA
1291 TGATCA
BglI 3405 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6073 GCCCTCTGGGC
BsaWI 1241 TCCGGT [AT]^CCGG[AT]
5589 TCCGGT
BsrBI 2012 CCGCTC CCGCTC(-3/-3)
114 GAGCGG
BsrFI 5784 GCCGGT [GA]^CCGG[CT]
5898 GCCGGC
BstUI 27 CGCG CG^CG
3011 CGCG
Cfr10I 5784 GCCGGT [GA]^CCGG[CT]
5898 GCCGGC
Eco47III 193 AGCGCT AGC^GCT
2759 AGCGCT
FbaI 1082 TGATCA T^GATCA
1291 TGATCA
KpnI 4947 GGTACC GGTAC^C
6174 GGTACC
MvnI 27 CGCG CG^CG
3011 CGCG
NspI 669 ACATGT [GA]CATG^[CT]
3143 GCATGC
PaeR7I 197 CTCGAG C^TCGAG
5396 CTCGAG
SacI 3248 GAGCTC GAGCT^C
6372 GAGCTC
StuI 2142 AGGCCT AGG^CCT
4171 AGGCCT
XhoI 197 CTCGAG C^TCGAG
5396 CTCGAG
XmnI 1507 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4731 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII