################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AclI AgeI AhdI AscI AseI AsnI AspEI AspI AviII BamHI BbrPI BcgI BciVI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI Eam1105I EcoO65I EcoT22I FseI FspI HpaI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1599 GACGTC GACGT^C Acc65I 500 GGTACC G^GTACC AflII 2020 CTTAAG C^TTAAG Alw44I 180 GTGCAC G^TGCAC AlwNI 170 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2714 CCTTAGG CC^T[ACGT]AGG Aor51HI 3079 AGCGCT AGC^GCT ApaLI 180 GTGCAC G^TGCAC Asp700I 2169 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 500 GGTACC G^GTACC AvaI 1032 CCCGGG C^[CT]CG[GA]G AvrII 1826 CCTAGG C^CTAGG BbeI 1040 GGCGCC GGCGC^C BbvCI 1883 CCTCAGC CCTCAGC(-5/-2) BclI 2378 TGATCA T^GATCA BfrI 2020 CTTAAG C^TTAAG BglI 880 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1826 CCTAGG C^CTAGG BsaAI 309 TACGTA [CT]AC^GT[GA] BsiEI 1722 CGGCCG CG[GA][CT]^CG BsoBI 1032 CCCGGG C^[CT]CG[GA]G BspHI 585 TCATGA T^CATGA BsrBI 3128 GAGCGG CCGCTC(-3/-3) BsrDI 4312 GCAATG GCAATG(2/0) BstAPI 2528 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2714 CCTTAGG CC^T[ACGT]AGG DraIII 2776 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1722 CGGCCG C^GGCCG EclXI 1722 CGGCCG C^GGCCG Eco47III 3079 AGCGCT AGC^GCT Eco52I 1722 CGGCCG C^GGCCG Eco81I 2714 CCTTAGG CC^T[ACGT]AGG EcoNI 1337 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4324 GAATTC G^AATTC FbaI 2378 TGATCA T^GATCA HincII 2310 GTCAAC GT[CT]^[GA]AC HindII 2310 GTCAAC GT[CT]^[GA]AC HindIII 336 AAGCTT A^AGCTT KasI 1040 GGCGCC G^GCGCC KpnI 500 GGTACC GGTAC^C NaeI 615 GCCGGC GCC^GGC NarI 1040 GGCGCC GG^CGCC NcoI 2829 CCATGG C^CATGG NgoMIV 615 GCCGGC G^CCGGC RcaI 585 TCATGA T^CATGA SapI 3990 GAAGAGC GCTCTTC(1/4) ScaI 3970 AGTACT AGT^ACT SfoI 1040 GGCGCC GGC^GCC SmaI 1032 CCCGGG CCC^GGG SmlI 2020 CTTAAG C^T[CT][GA]AG SnaBI 309 TACGTA TAC^GTA SpeI 2314 ACTAGT A^CTAGT SphI 1990 GCATGC GCATG^C XcmI 3004 CCATCTTCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1032 CCCGGG C^CCGGG XmaI 1032 CCCGGG C^CCGGG XmnI 2169 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2074 GTCTAC GT^[AC][GT]AC 2922 GTCTAC AccII 2940 CGCG CG^CG 3233 CGCG AccIII 3124 TCCGGA T^CCGGA 3655 TCCGGA AcsI 2172 GAATTT [GA]^AATT[CT] 4324 GAATTC ApaI 1582 GGGCCC GGGCC^C 4027 GGGCCC ApoI 2172 GAATTT [GA]^AATT[CT] 4324 GAATTC BalI 826 TGGCCA TGG^CCA 1806 TGGCCA BbsI 640 GAAGAC GAAGAC(2/6) 1387 GTCTTC BpuAI 640 GAAGAC GAAGAC(2/6) 1387 GTCTTC BseAI 3124 TCCGGA T^CCGGA 3655 TCCGGA BsgI 1075 GTGCAG GTGCAG(16/14) 1647 GTGCAG BsmBI 4032 CGTCTC CGTCTC(1/5) 1060 GAGACG BspEI 3124 TCCGGA T^CCGGA 3655 TCCGGA BssSI 927 CACGAG CACGAG(-5/-1) 1775 CACGAG BstUI 2940 CGCG CG^CG 3233 CGCG BstXI 2672 CCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3336 CCAGGGGACTGG DraI 1126 TTTAAA TTT^AAA 3600 TTTAAA EcoRV 82 GATATC GAT^ATC 2219 GATATC HgaI 2273 GCGTC GACGC(5/10) 3234 GCGTC MluNI 826 TGGCCA TGG^CCA 1806 TGGCCA MroI 3124 TCCGGA T^CCGGA 3655 TCCGGA MscI 826 TGGCCA TGG^CCA 1806 TGGCCA MvnI 2940 CGCG CG^CG 3233 CGCG PflMI 357 CCAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 799 CCAAGCGGTGG SspI 4058 AATATT AAT^ATT 4165 AATATT Van91I 357 CCAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 799 CCAAGCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII