##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AclI AgeI AhdI AscI AseI
AsnI AspEI AspI AviII BamHI
BbrPI BcgI BciVI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI Eam1105I
EcoO65I EcoT22I FseI FspI HpaI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI Sse8387I SspBI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1599 GACGTC GACGT^C
Acc65I 500 GGTACC G^GTACC
AflII 2020 CTTAAG C^TTAAG
Alw44I 180 GTGCAC G^TGCAC
AlwNI 170 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2714 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3079 AGCGCT AGC^GCT
ApaLI 180 GTGCAC G^TGCAC
Asp700I 2169 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 500 GGTACC G^GTACC
AvaI 1032 CCCGGG C^[CT]CG[GA]G
AvrII 1826 CCTAGG C^CTAGG
BbeI 1040 GGCGCC GGCGC^C
BbvCI 1883 CCTCAGC CCTCAGC(-5/-2)
BclI 2378 TGATCA T^GATCA
BfrI 2020 CTTAAG C^TTAAG
BglI 880 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1826 CCTAGG C^CTAGG
BsaAI 309 TACGTA [CT]AC^GT[GA]
BsiEI 1722 CGGCCG CG[GA][CT]^CG
BsoBI 1032 CCCGGG C^[CT]CG[GA]G
BspHI 585 TCATGA T^CATGA
BsrBI 3128 GAGCGG CCGCTC(-3/-3)
BsrDI 4312 GCAATG GCAATG(2/0)
BstAPI 2528 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2714 CCTTAGG CC^T[ACGT]AGG
DraIII 2776 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1722 CGGCCG C^GGCCG
EclXI 1722 CGGCCG C^GGCCG
Eco47III 3079 AGCGCT AGC^GCT
Eco52I 1722 CGGCCG C^GGCCG
Eco81I 2714 CCTTAGG CC^T[ACGT]AGG
EcoNI 1337 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4324 GAATTC G^AATTC
FbaI 2378 TGATCA T^GATCA
HincII 2310 GTCAAC GT[CT]^[GA]AC
HindII 2310 GTCAAC GT[CT]^[GA]AC
HindIII 336 AAGCTT A^AGCTT
KasI 1040 GGCGCC G^GCGCC
KpnI 500 GGTACC GGTAC^C
NaeI 615 GCCGGC GCC^GGC
NarI 1040 GGCGCC GG^CGCC
NcoI 2829 CCATGG C^CATGG
NgoMIV 615 GCCGGC G^CCGGC
RcaI 585 TCATGA T^CATGA
SapI 3990 GAAGAGC GCTCTTC(1/4)
ScaI 3970 AGTACT AGT^ACT
SfoI 1040 GGCGCC GGC^GCC
SmaI 1032 CCCGGG CCC^GGG
SmlI 2020 CTTAAG C^T[CT][GA]AG
SnaBI 309 TACGTA TAC^GTA
SpeI 2314 ACTAGT A^CTAGT
SphI 1990 GCATGC GCATG^C
XcmI 3004 CCATCTTCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1032 CCCGGG C^CCGGG
XmaI 1032 CCCGGG C^CCGGG
XmnI 2169 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2074 GTCTAC GT^[AC][GT]AC
2922 GTCTAC
AccII 2940 CGCG CG^CG
3233 CGCG
AccIII 3124 TCCGGA T^CCGGA
3655 TCCGGA
AcsI 2172 GAATTT [GA]^AATT[CT]
4324 GAATTC
ApaI 1582 GGGCCC GGGCC^C
4027 GGGCCC
ApoI 2172 GAATTT [GA]^AATT[CT]
4324 GAATTC
BalI 826 TGGCCA TGG^CCA
1806 TGGCCA
BbsI 640 GAAGAC GAAGAC(2/6)
1387 GTCTTC
BpuAI 640 GAAGAC GAAGAC(2/6)
1387 GTCTTC
BseAI 3124 TCCGGA T^CCGGA
3655 TCCGGA
BsgI 1075 GTGCAG GTGCAG(16/14)
1647 GTGCAG
BsmBI 4032 CGTCTC CGTCTC(1/5)
1060 GAGACG
BspEI 3124 TCCGGA T^CCGGA
3655 TCCGGA
BssSI 927 CACGAG CACGAG(-5/-1)
1775 CACGAG
BstUI 2940 CGCG CG^CG
3233 CGCG
BstXI 2672 CCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3336 CCAGGGGACTGG
DraI 1126 TTTAAA TTT^AAA
3600 TTTAAA
EcoRV 82 GATATC GAT^ATC
2219 GATATC
HgaI 2273 GCGTC GACGC(5/10)
3234 GCGTC
MluNI 826 TGGCCA TGG^CCA
1806 TGGCCA
MroI 3124 TCCGGA T^CCGGA
3655 TCCGGA
MscI 826 TGGCCA TGG^CCA
1806 TGGCCA
MvnI 2940 CGCG CG^CG
3233 CGCG
PflMI 357 CCAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
799 CCAAGCGGTGG
SspI 4058 AATATT AAT^ATT
4165 AATATT
Van91I 357 CCAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
799 CCAAGCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII