##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AflII AgeI Aor51HI
AscI Asp700I AspI AviII AvrII
BcgI BfrI BlnI BsaI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI Eco47III
EcoO65I EcoRV FseI FspI HpaI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PvuI RcaI
RsrII SalI SbfI ScaI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 126 AACGTT AA^CGTT
ApaI 4485 GGGCCC GGGCC^C
AseI 136 ATTAAT AT^TAAT
AsnI 136 ATTAAT AT^TAAT
BbrPI 1657 CACGTG CAC^GTG
BbvCI 2671 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1657 CACGTG [CT]AC^GT[GA]
BsiEI 716 CGGCCG CG[GA][CT]^CG
BsmBI 251 GAGACG CGTCTC(1/5)
BsrDI 1097 CATTGC GCAATG(2/0)
BsrFI 714 GCCGGC [GA]^CCGG[CT]
BssSI 3669 CACGAG CACGAG(-5/-1)
BstAPI 1538 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1701 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 714 GCCGGC [GA]^CCGG[CT]
DraI 4798 TTTAAA TTT^AAA
DraIII 1404 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 716 CGGCCG C^GGCCG
EclXI 716 CGGCCG C^GGCCG
Eco52I 716 CGGCCG C^GGCCG
EcoRI 3343 GAATTC G^AATTC
HgaI 2108 GACGC GACGC(5/10)
HincII 197 GTCAAC GT[CT]^[GA]AC
HindII 197 GTCAAC GT[CT]^[GA]AC
HindIII 426 AAGCTT A^AGCTT
KspI 668 CCGCGG CCGC^GG
NaeI 714 GCCGGC GCC^GGC
NcoI 4297 CCATGG C^CATGG
NgoMIV 714 GCCGGC G^CCGGC
NheI 1389 GCTAGC G^CTAGC
PflMI 326 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1657 CACGTG CAC^GTG
PmlI 1657 CACGTG CAC^GTG
PshBI 136 ATTAAT AT^TAAT
Psp1406I 126 AACGTT AA^CGTT
PstI 3730 CTGCAG CTGCA^G
SacII 668 CCGCGG CCGC^GG
SexAI 4595 ACCTGGT A^CC[AT]GGT
SfiI 717 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 90 ACTAGT A^CTAGT
Van91I 326 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 963 CCAGAGAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1398 GTCTAC GT^[AC][GT]AC
1892 GTAGAC
AccII 669 CGCG CG^CG
3546 CGCG
AhdI 551 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
999 GACCTTGTGTC
Alw44I 1654 GTGCAC G^TGCAC
4018 GTGCAC
AocI 680 CCTCAGG CC^T[ACGT]AGG
3437 CCTGAGG
ApaLI 1654 GTGCAC G^TGCAC
4018 GTGCAC
AspEI 551 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
999 GACCTTGTGTC
BbeI 751 GGCGCC GGCGC^C
4260 GGCGCC
BclI 1480 TGATCA T^GATCA
5127 TGATCA
BlpI 3675 GCTAAGC GC^T[ACGT]AGC
4122 GCTCAGC
Bpu1102I 3675 GCTAAGC GC^T[ACGT]AGC
4122 GCTCAGC
BsaBI 348 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
804 GATTTTTATC
BspLU11I 1306 ACATGT A^CATGT
2990 ACATGT
BstUI 669 CGCG CG^CG
3546 CGCG
Bsu36I 680 CCTCAGG CC^T[ACGT]AGG
3437 CCTGAGG
CelII 3675 GCTAAGC GC^T[ACGT]AGC
4122 GCTCAGC
Eam1105I 551 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
999 GACCTTGTGTC
Eco81I 680 CCTCAGG CC^T[ACGT]AGG
3437 CCTGAGG
EcoNI 1689 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4342 CCTCCCACAGG
EcoT22I 2414 ATGCAT ATGCA^T
3488 ATGCAT
FbaI 1480 TGATCA T^GATCA
5127 TGATCA
HaeII 751 GGCGCC [GA]GCGC^[CT]
4260 GGCGCC
KasI 751 GGCGCC G^GCGCC
4260 GGCGCC
MamI 348 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
804 GATTTTTATC
MvnI 669 CGCG CG^CG
3546 CGCG
NarI 751 GGCGCC GG^CGCC
4260 GGCGCC
NsiI 2414 ATGCAT ATGCA^T
3488 ATGCAT
PpuMI 2031 AGGTCCT [GA]G^G[AT]CC[CT]
3568 GGGTCCT
PshAI 190 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
947 GACTGGAGTC
SacI 4120 GAGCTC GAGCT^C
4945 GAGCTC
SapI 1964 GAAGAGC GCTCTTC(1/4)
3120 GAAGAGC
SfoI 751 GGCGCC GGC^GCC
4260 GGCGCC
SmaI 3691 CCCGGG CCC^GGG
4995 CCCGGG
SmlI 69 CTCAAG C^T[CT][GA]AG
3996 CTTGAG
SspI 4774 AATATT AAT^ATT
5072 AATATT
StuI 1334 AGGCCT AGG^CCT
2668 AGGCCT
XbaI 318 TCTAGA T^CTAGA
4941 TCTAGA
XmaCI 3691 CCCGGG C^CCGGG
4995 CCCGGG
XmaI 3691 CCCGGG C^CCGGG
4995 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BciVI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII