##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaLI AscI AviII AvrII BalI
BbeI BcgI BfrI BlnI BsaWI
BseAI BsiWI BsmI Bsp1407I BspEI
BsrGI BssHII CpoI DraIII EcoRV
FseI FspI KasI KspI MluI
MluNI MroI MscI NarI NheI
NotI NruI PacI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI Sse8387I SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 3354 GACGTC GACGT^C
Acc65I 1624 GGTACC G^GTACC
AhdI 1251 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4914 CCTCAGG CC^T[ACGT]AGG
Aor51HI 5024 AGCGCT AGC^GCT
ApaI 1612 GGGCCC GGGCC^C
AseI 5337 ATTAAT AT^TAAT
AsnI 5337 ATTAAT AT^TAAT
Asp718I 1624 GGTACC G^GTACC
AspEI 1251 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2309 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3293 GGATAC GTATCC(6/5)
BclI 1109 TGATCA T^GATCA
BglI 109 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4974 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 5238 GTGCAG GTGCAG(16/14)
BsmBI 3686 GAGACG CGTCTC(1/5)
BspHI 4594 TCATGA T^CATGA
BspLU11I 1774 ACATGT A^CATGT
BsrFI 4791 GCCGGC [GA]^CCGG[CT]
Bst1107I 5697 GTATAC GTA^TAC
BstAPI 4956 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 166 GGTAACC G^GT[ACGT]ACC
BstPI 166 GGTAACC G^GT[ACGT]ACC
BstZ17I 5697 GTATAC GTA^TAC
Bsu36I 4914 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4791 GCCGGC [GA]^CCGG[CT]
DrdI 2991 GACCGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4635 CGGCCG C^GGCCG
Eam1105I 1251 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4635 CGGCCG C^GGCCG
Eco47III 5024 AGCGCT AGC^GCT
Eco52I 4635 CGGCCG C^GGCCG
Eco81I 4914 CCTCAGG CC^T[ACGT]AGG
EcoO65I 166 GGTAACC G^GT[ACGT]ACC
EcoT22I 5767 ATGCAT ATGCA^T
FbaI 1109 TGATCA T^GATCA
HincII 2720 GTTAAC GT[CT]^[GA]AC
HindII 2720 GTTAAC GT[CT]^[GA]AC
HpaI 2720 GTTAAC GTT^AAC
KpnI 1624 GGTACC GGTAC^C
MamI 4974 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5556 CAATTG C^AATTG
MunI 5556 CAATTG C^AATTG
NaeI 4791 GCCGGC GCC^GGC
NgoMIV 4791 GCCGGC G^CCGGC
NsiI 5767 ATGCAT ATGCA^T
PaeR7I 2227 CTCGAG C^TCGAG
PflFI 2309 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1123 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 5337 ATTAAT AT^TAAT
RcaI 4594 TCATGA T^CATGA
SpeI 2322 ACTAGT A^CTAGT
SphI 1033 GCATGC GCATG^C
SspI 4399 AATATT AAT^ATT
Tth111I 2309 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1123 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2227 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1030 CGCG CG^CG
2494 CGCG
AcyI 3137 GACGCC G[GA]^CG[CT]C
3354 GACGTC
BamHI 4540 GGATCC G^GATCC
5479 GGATCC
BbrPI 3899 CACGTG CAC^GTG
5722 CACGTG
BsaHI 3137 GACGCC G[GA]^CG[CT]C
3354 GACGTC
BsiEI 2990 CGACCG CG[GA][CT]^CG
4635 CGGCCG
BspDI 690 ATCGAT AT^CGAT
3399 ATCGAT
BsrBI 3699 GAGCGG CCGCTC(-3/-3)
3943 GAGCGG
BsrDI 5130 CATTGC GCAATG(2/0)
4956 GCAATG
BssSI 864 CACGAG CACGAG(-5/-1)
3516 CACGAG
BstUI 1030 CGCG CG^CG
2494 CGCG
ClaI 690 ATCGAT AT^CGAT
3399 ATCGAT
EcoRI 3511 GAATTC G^AATTC
3806 GAATTC
Hin1I 3137 GACGCC G[GA]^CG[CT]C
3354 GACGTC
MslI 1028 CACGCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5156 CACTGTGGTG
MvnI 1030 CGCG CG^CG
2494 CGCG
NcoI 1182 CCATGG C^CATGG
5688 CCATGG
NdeI 5331 CATATG CA^TATG
5484 CATATG
PmaCI 3899 CACGTG CAC^GTG
5722 CACGTG
PmlI 3899 CACGTG CAC^GTG
5722 CACGTG
PshAI 3993 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4707 GACTTCAGTC
SacI 1100 GAGCTC GAGCT^C
5727 GAGCTC
XcmI 104 CCACCGCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2932 CCACAGAAGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstBI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI NspV PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI
XmnI