################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AccIII AclI AflII AgeI Alw44I ApaLI AscI AviII AvrII BalI BbeI BcgI BfrI BlnI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BsrGI BssHII CpoI DraIII EcoRV FseI FspI KasI KspI MluI MluNI MroI MscI NarI NheI NotI NruI PacI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI Sse8387I SspBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 3354 GACGTC GACGT^C Acc65I 1624 GGTACC G^GTACC AhdI 1251 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 4914 CCTCAGG CC^T[ACGT]AGG Aor51HI 5024 AGCGCT AGC^GCT ApaI 1612 GGGCCC GGGCC^C AseI 5337 ATTAAT AT^TAAT AsnI 5337 ATTAAT AT^TAAT Asp718I 1624 GGTACC G^GTACC AspEI 1251 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2309 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 3293 GGATAC GTATCC(6/5) BclI 1109 TGATCA T^GATCA BglI 109 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4974 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 5238 GTGCAG GTGCAG(16/14) BsmBI 3686 GAGACG CGTCTC(1/5) BspHI 4594 TCATGA T^CATGA BspLU11I 1774 ACATGT A^CATGT BsrFI 4791 GCCGGC [GA]^CCGG[CT] Bst1107I 5697 GTATAC GTA^TAC BstAPI 4956 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 166 GGTAACC G^GT[ACGT]ACC BstPI 166 GGTAACC G^GT[ACGT]ACC BstZ17I 5697 GTATAC GTA^TAC Bsu36I 4914 CCTCAGG CC^T[ACGT]AGG Cfr10I 4791 GCCGGC [GA]^CCGG[CT] DrdI 2991 GACCGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4635 CGGCCG C^GGCCG Eam1105I 1251 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4635 CGGCCG C^GGCCG Eco47III 5024 AGCGCT AGC^GCT Eco52I 4635 CGGCCG C^GGCCG Eco81I 4914 CCTCAGG CC^T[ACGT]AGG EcoO65I 166 GGTAACC G^GT[ACGT]ACC EcoT22I 5767 ATGCAT ATGCA^T FbaI 1109 TGATCA T^GATCA HincII 2720 GTTAAC GT[CT]^[GA]AC HindII 2720 GTTAAC GT[CT]^[GA]AC HpaI 2720 GTTAAC GTT^AAC KpnI 1624 GGTACC GGTAC^C MamI 4974 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5556 CAATTG C^AATTG MunI 5556 CAATTG C^AATTG NaeI 4791 GCCGGC GCC^GGC NgoMIV 4791 GCCGGC G^CCGGC NsiI 5767 ATGCAT ATGCA^T PaeR7I 2227 CTCGAG C^TCGAG PflFI 2309 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1123 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 5337 ATTAAT AT^TAAT RcaI 4594 TCATGA T^CATGA SpeI 2322 ACTAGT A^CTAGT SphI 1033 GCATGC GCATG^C SspI 4399 AATATT AAT^ATT Tth111I 2309 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1123 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2227 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 1030 CGCG CG^CG 2494 CGCG AcyI 3137 GACGCC G[GA]^CG[CT]C 3354 GACGTC BamHI 4540 GGATCC G^GATCC 5479 GGATCC BbrPI 3899 CACGTG CAC^GTG 5722 CACGTG BsaHI 3137 GACGCC G[GA]^CG[CT]C 3354 GACGTC BsiEI 2990 CGACCG CG[GA][CT]^CG 4635 CGGCCG BspDI 690 ATCGAT AT^CGAT 3399 ATCGAT BsrBI 3699 GAGCGG CCGCTC(-3/-3) 3943 GAGCGG BsrDI 5130 CATTGC GCAATG(2/0) 4956 GCAATG BssSI 864 CACGAG CACGAG(-5/-1) 3516 CACGAG BstUI 1030 CGCG CG^CG 2494 CGCG ClaI 690 ATCGAT AT^CGAT 3399 ATCGAT EcoRI 3511 GAATTC G^AATTC 3806 GAATTC Hin1I 3137 GACGCC G[GA]^CG[CT]C 3354 GACGTC MslI 1028 CACGCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 5156 CACTGTGGTG MvnI 1030 CGCG CG^CG 2494 CGCG NcoI 1182 CCATGG C^CATGG 5688 CCATGG NdeI 5331 CATATG CA^TATG 5484 CATATG PmaCI 3899 CACGTG CAC^GTG 5722 CACGTG PmlI 3899 CACGTG CAC^GTG 5722 CACGTG PshAI 3993 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4707 GACTTCAGTC SacI 1100 GAGCTC GAGCT^C 5727 GAGCTC XcmI 104 CCACCGCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2932 CCACAGAAGATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstBI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI NspV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI