##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI Asp718I
AviII AvrII BamHI BbvCI BcnI
BfrI BglI BlnI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BstAPI BstEII
BstPI Cfr10I CpoI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI FspI HpaI KpnI KspI
MamI MluI MroI NaeI NciI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 4173 GTATAC GT^[AC][GT]AC
AccII 1459 CGCG CG^CG
Alw44I 3626 GTGCAC G^TGCAC
AocI 3243 CCTAAGG CC^T[ACGT]AGG
ApaLI 3626 GTGCAC G^TGCAC
AspI 2551 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3115 CCCGAG C^[CT]CG[GA]G
BalI 3636 TGGCCA TGG^CCA
BbeI 3363 GGCGCC GGCGC^C
BbrPI 3672 CACGTG CAC^GTG
BcgI 2772 CGATGGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2565 TGATCA T^GATCA
BlpI 2893 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2893 GCTAAGC GC^T[ACGT]AGC
BsaWI 1718 ACCGGA [AT]^CCGG[AT]
BseRI 2684 CTCCTC GAGGAG(10/8)
BsoBI 3115 CCCGAG C^[CT]CG[GA]G
BspDI 1790 ATCGAT AT^CGAT
BspMI 4270 ACCTGC ACCTGC(4/8)
BssSI 2497 CTCGTG CACGAG(-5/-1)
Bst1107I 4173 GTATAC GTA^TAC
BstUI 1459 CGCG CG^CG
BstZ17I 4173 GTATAC GTA^TAC
Bsu36I 3243 CCTAAGG CC^T[ACGT]AGG
CelII 2893 GCTAAGC GC^T[ACGT]AGC
ClaI 1790 ATCGAT AT^CGAT
DrdI 4431 GACTGTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3636 TGGCCA [CT]^GGCC[GA]
Eco81I 3243 CCTAAGG CC^T[ACGT]AGG
EcoRI 4321 GAATTC G^AATTC
FauI 4345 CCCGC CCCGC(4/6)
FbaI 2565 TGATCA T^GATCA
HincII 2913 GTCAAC GT[CT]^[GA]AC
HindII 2913 GTCAAC GT[CT]^[GA]AC
KasI 3363 GGCGCC G^GCGCC
MfeI 3755 CAATTG C^AATTG
MluNI 3636 TGGCCA TGG^CCA
MscI 3636 TGGCCA TGG^CCA
MunI 3755 CAATTG C^AATTG
MvnI 1459 CGCG CG^CG
NarI 3363 GGCGCC GG^CGCC
NcoI 2783 CCATGG C^CATGG
NheI 4130 GCTAGC G^CTAGC
PflFI 2551 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3672 CACGTG CAC^GTG
PmlI 3672 CACGTG CAC^GTG
PstI 3131 CTGCAG CTGCA^G
SfoI 3363 GGCGCC GGC^GCC
SphI 4569 GCATGC GCATG^C
StuI 416 AGGCCT AGG^CCT
Tth111I 2551 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 753 TCTAGA T^CTAGA
XcmI 4867 CCAGCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 3363 GGCGCC G[GA]^CG[CT]C
4910 GACGCC
AflIII 1013 ACATGT A^C[GA][CT]GT
1526 ACATGT
AhdI 1967 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2966 GACTGGAGGTC
Asp700I 1357 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1722 GAACCCCTTC
AspEI 1967 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2966 GACTGGAGGTC
BanI 669 GGTGCC G^G[CT][GA]CC
3363 GGCGCC
BglII 2419 AGATCT A^GATCT
4904 AGATCT
BsaAI 2154 CACGTA [CT]AC^GT[GA]
3672 CACGTG
BsaHI 3363 GGCGCC G[GA]^CG[CT]C
4910 GACGCC
BsmI 503 GCATTC GAATGC(1/-1)
1566 GCATTC
Bsp1407I 134 TGTACA T^GTACA
287 TGTACA
BspLU11I 1013 ACATGT A^CATGT
1526 ACATGT
BsrGI 134 TGTACA T^GTACA
287 TGTACA
BstBI 2111 TTCGAA TT^CGAA
2636 TTCGAA
DraII 153 AGGACCC [GA]G^G[ACGT]CC[CT]
3812 GGGACCC
DsaI 2783 CCATGG C^C[GA][CT]GG
3140 CCGTGG
Eam1105I 1967 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2966 GACTGGAGGTC
EcoO109I 153 AGGACCC [GA]G^G[ACGT]CC[CT]
3812 GGGACCC
EcoT22I 968 ATGCAT ATGCA^T
1564 ATGCAT
HaeII 1752 GGCGCT [GA]GCGC^[CT]
3363 GGCGCC
HapII 1719 CCGG C^CGG
2464 CCGG
HgaI 4910 GACGC GACGC(5/10)
1227 GCGTC
Hin1I 3363 GGCGCC G[GA]^CG[CT]C
4910 GACGCC
HindIII 535 AAGCTT A^AGCTT
2806 AAGCTT
HpaII 1719 CCGG C^CGG
2464 CCGG
MspI 1719 CCGG C^CGG
2464 CCGG
NdeI 1093 CATATG CA^TATG
3508 CATATG
NsiI 968 ATGCAT ATGCA^T
1564 ATGCAT
NspV 2111 TTCGAA TT^CGAA
2636 TTCGAA
PpuMI 153 AGGACCC [GA]G^G[AT]CC[CT]
3812 GGGACCC
SacI 858 GAGCTC GAGCT^C
2494 GAGCTC
SfuI 2111 TTCGAA TT^CGAA
2636 TTCGAA
SpeI 2917 ACTAGT A^CTAGT
4037 ACTAGT
SspBI 134 TGTACA T^GTACA
287 TGTACA
XmnI 1357 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1722 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanII BbsI BbvI BciVI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraIII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII