################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI BcgI BfrI BsiEI BsiWI BsmBI BspDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV FseI HindIII HpaI KspI MfeI MluI MunI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 4127 GGTACC G^GTACC AccIII 4714 TCCGGA T^CCGGA AcsI 677 GAATTT [GA]^AATT[CT] AhdI 218 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 677 GAATTT [GA]^AATT[CT] Asp700I 1306 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4127 GGTACC G^GTACC AspEI 218 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4650 CCTAGG C^CTAGG BamHI 3270 GGATCC G^GATCC BbrPI 1329 CACGTG CAC^GTG BglI 3222 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4650 CCTAGG C^CTAGG BlpI 758 GCTCAGC GC^T[ACGT]AGC Bpu1102I 758 GCTCAGC GC^T[ACGT]AGC BsaAI 1329 CACGTG [CT]AC^GT[GA] BsaBI 4115 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4714 TCCGGA [AT]^CCGG[AT] BseAI 4714 TCCGGA T^CCGGA Bsp1407I 534 TGTACA T^GTACA BspEI 4714 TCCGGA T^CCGGA BspHI 4511 TCATGA T^CATGA BspLU11I 4070 ACATGT A^CATGT BsrGI 534 TGTACA T^GTACA BssHII 1390 GCGCGC G^CGCGC BstAPI 2852 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3857 GGTTACC G^GT[ACGT]ACC BstPI 3857 GGTTACC G^GT[ACGT]ACC CelII 758 GCTCAGC GC^T[ACGT]AGC DraI 1022 TTTAAA TTT^AAA Eam1105I 218 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3857 GGTTACC G^GT[ACGT]ACC KpnI 4127 GGTACC GGTAC^C MamI 4115 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 4714 TCCGGA T^CCGGA NaeI 1881 GCCGGC GCC^GGC NgoMIV 1881 GCCGGC G^CCGGC NheI 1076 GCTAGC G^CTAGC PmaCI 1329 CACGTG CAC^GTG PmlI 1329 CACGTG CAC^GTG RcaI 4511 TCATGA T^CATGA SexAI 5006 ACCAGGT A^CC[AT]GGT SgrAI 1393 CGCCGGTG C[GA]^CCGG[CT]G SmaI 3797 CCCGGG CCC^GGG SspBI 534 TGTACA T^GTACA TaqI 2842 TCGA T^CGA TthHB8I 2842 TCGA T^CGA XmaCI 3797 CCCGGG C^CCGGG XmaI 3797 CCCGGG C^CCGGG XmnI 1306 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 4216 GTCTAC GT^[AC][GT]AC 4394 GTAGAC AflIII 1328 ACACGT A^C[GA][CT]GT 4070 ACATGT AviII 1653 TGCGCA TGC^GCA 2162 TGCGCA BclI 495 TGATCA T^GATCA 2661 TGATCA BglII 741 AGATCT A^GATCT 5100 AGATCT BsrDI 4094 CATTGC GCAATG(2/0) 3814 GCAATG DrdI 158 GACGCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3283 GACTGTACTGTC EcoT22I 2321 ATGCAT ATGCA^T 3434 ATGCAT FbaI 495 TGATCA T^GATCA 2661 TGATCA FspI 1653 TGCGCA TGC^GCA 2162 TGCGCA NsiI 2321 ATGCAT ATGCA^T 3434 ATGCAT SacI 2041 GAGCTC GAGCT^C 4515 GAGCTC ScaI 2742 AGTACT AGT^ACT 4290 AGTACT XcmI 220 CCAGAAGTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 918 CCAGTAGCCAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XhoII