##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI BcgI BfrI BsiEI BsiWI
BsmBI BspDI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HindIII HpaI KspI MfeI MluI
MunI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 4127 GGTACC G^GTACC
AccIII 4714 TCCGGA T^CCGGA
AcsI 677 GAATTT [GA]^AATT[CT]
AhdI 218 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 677 GAATTT [GA]^AATT[CT]
Asp700I 1306 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4127 GGTACC G^GTACC
AspEI 218 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4650 CCTAGG C^CTAGG
BamHI 3270 GGATCC G^GATCC
BbrPI 1329 CACGTG CAC^GTG
BglI 3222 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4650 CCTAGG C^CTAGG
BlpI 758 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 758 GCTCAGC GC^T[ACGT]AGC
BsaAI 1329 CACGTG [CT]AC^GT[GA]
BsaBI 4115 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4714 TCCGGA [AT]^CCGG[AT]
BseAI 4714 TCCGGA T^CCGGA
Bsp1407I 534 TGTACA T^GTACA
BspEI 4714 TCCGGA T^CCGGA
BspHI 4511 TCATGA T^CATGA
BspLU11I 4070 ACATGT A^CATGT
BsrGI 534 TGTACA T^GTACA
BssHII 1390 GCGCGC G^CGCGC
BstAPI 2852 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3857 GGTTACC G^GT[ACGT]ACC
BstPI 3857 GGTTACC G^GT[ACGT]ACC
CelII 758 GCTCAGC GC^T[ACGT]AGC
DraI 1022 TTTAAA TTT^AAA
Eam1105I 218 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3857 GGTTACC G^GT[ACGT]ACC
KpnI 4127 GGTACC GGTAC^C
MamI 4115 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4714 TCCGGA T^CCGGA
NaeI 1881 GCCGGC GCC^GGC
NgoMIV 1881 GCCGGC G^CCGGC
NheI 1076 GCTAGC G^CTAGC
PmaCI 1329 CACGTG CAC^GTG
PmlI 1329 CACGTG CAC^GTG
RcaI 4511 TCATGA T^CATGA
SexAI 5006 ACCAGGT A^CC[AT]GGT
SgrAI 1393 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 3797 CCCGGG CCC^GGG
SspBI 534 TGTACA T^GTACA
TaqI 2842 TCGA T^CGA
TthHB8I 2842 TCGA T^CGA
XmaCI 3797 CCCGGG C^CCGGG
XmaI 3797 CCCGGG C^CCGGG
XmnI 1306 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 4216 GTCTAC GT^[AC][GT]AC
4394 GTAGAC
AflIII 1328 ACACGT A^C[GA][CT]GT
4070 ACATGT
AviII 1653 TGCGCA TGC^GCA
2162 TGCGCA
BclI 495 TGATCA T^GATCA
2661 TGATCA
BglII 741 AGATCT A^GATCT
5100 AGATCT
BsrDI 4094 CATTGC GCAATG(2/0)
3814 GCAATG
DrdI 158 GACGCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3283 GACTGTACTGTC
EcoT22I 2321 ATGCAT ATGCA^T
3434 ATGCAT
FbaI 495 TGATCA T^GATCA
2661 TGATCA
FspI 1653 TGCGCA TGC^GCA
2162 TGCGCA
NsiI 2321 ATGCAT ATGCA^T
3434 ATGCAT
SacI 2041 GAGCTC GAGCT^C
4515 GAGCTC
ScaI 2742 AGTACT AGT^ACT
4290 AGTACT
XcmI 220 CCAGAAGTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
918 CCAGTAGCCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XhoII