##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Aor51HI ApaI AscI AvrII
BbeI BbrPI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I FseI
HaeII HincII HindII HpaI KasI
KspI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 223 GTCTAC GT^[AC][GT]AC
Alw44I 2542 GTGCAC G^TGCAC
AocI 2307 CCTGAGG CC^T[ACGT]AGG
ApaLI 2542 GTGCAC G^TGCAC
AseI 299 ATTAAT AT^TAAT
AsnI 299 ATTAAT AT^TAAT
Asp700I 5014 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1503 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5040 TGCGCA TGC^GCA
BamHI 53 GGATCC G^GATCC
BbvCI 4181 GCTGAGG CCTCAGC(-5/-2)
BclI 2405 TGATCA T^GATCA
BglI 1212 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 5087 CACGTA [CT]AC^GT[GA]
BsaWI 106 ACCGGA [AT]^CCGG[AT]
BsiEI 1630 CGATCG CG[GA][CT]^CG
BsrDI 3694 CATTGC GCAATG(2/0)
BstEII 870 GGTGACC G^GT[ACGT]ACC
BstPI 870 GGTGACC G^GT[ACGT]ACC
BstXI 1364 CCAGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2307 CCTGAGG CC^T[ACGT]AGG
DrdI 2873 GACTGTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2307 CCTGAGG CC^T[ACGT]AGG
EcoO65I 870 GGTGACC G^GT[ACGT]ACC
EcoT22I 3490 ATGCAT ATGCA^T
FbaI 2405 TGATCA T^GATCA
FspI 5040 TGCGCA TGC^GCA
NaeI 1577 GCCGGC GCC^GGC
NgoMIV 1577 GCCGGC G^CCGGC
NsiI 3490 ATGCAT ATGCA^T
PflFI 1503 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 216 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 299 ATTAAT AT^TAAT
PvuI 1630 CGATCG CGAT^CG
PvuII 3742 CAGCTG CAG^CTG
SmaI 460 CCCGGG CCC^GGG
StuI 3333 AGGCCT AGG^CCT
Tth111I 1503 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1140 TCTAGA T^CTAGA
XcmI 592 CCAATGAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 460 CCCGGG C^CCGGG
XmaI 460 CCCGGG C^CCGGG
XmnI 5014 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 536 CGCG CG^CG
1615 CGCG
AhdI 1995 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4685 GACAGTGTGTC
AspEI 1995 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4685 GACAGTGTGTC
BsaBI 2661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2933 GATGATGATC
BsgI 495 GTGCAG GTGCAG(16/14)
1837 GTGCAG
BsmI 5014 GAATGC GAATGC(1/-1)
3755 GCATTC
BspHI 272 TCATGA T^CATGA
4702 TCATGA
BsrBI 126 GAGCGG CCGCTC(-3/-3)
597 GAGCGG
BstUI 536 CGCG CG^CG
1615 CGCG
DraIII 4982 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5087 CACGTAGTG
Eam1105I 1995 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4685 GACAGTGTGTC
EcoRI 243 GAATTC G^AATTC
566 GAATTC
EcoRV 987 GATATC GAT^ATC
4811 GATATC
MamI 2661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2933 GATGATGATC
MspA1I 999 CAGCGG C[AC]G^C[GT]G
3742 CAGCTG
MvnI 536 CGCG CG^CG
1615 CGCG
NspI 5037 GCATGC [GA]CATG^[CT]
5046 GCATGC
PflMI 2900 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4003 CCACATCCTGG
RcaI 272 TCATGA T^CATGA
4702 TCATGA
SacI 1359 GAGCTC GAGCT^C
1394 GAGCTC
SmlI 423 CTCAAG C^T[CT][GA]AG
3600 CTCAAG
SphI 5037 GCATGC GCATG^C
5046 GCATGC
SspI 1962 AATATT AAT^ATT
4805 AATATT
TaqI 3498 TCGA T^CGA
4150 TCGA
TthHB8I 3498 TCGA T^CGA
4150 TCGA
Van91I 2900 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4003 CCACATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII