################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI Aor51HI ApaI AscI AvrII BbeI BbrPI BcgI BciVI BfrI BlnI BlpI Bpu1102I BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI EagI EclXI Eco47III Eco52I FseI HaeII HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 223 GTCTAC GT^[AC][GT]AC Alw44I 2542 GTGCAC G^TGCAC AocI 2307 CCTGAGG CC^T[ACGT]AGG ApaLI 2542 GTGCAC G^TGCAC AseI 299 ATTAAT AT^TAAT AsnI 299 ATTAAT AT^TAAT Asp700I 5014 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1503 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5040 TGCGCA TGC^GCA BamHI 53 GGATCC G^GATCC BbvCI 4181 GCTGAGG CCTCAGC(-5/-2) BclI 2405 TGATCA T^GATCA BglI 1212 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 5087 CACGTA [CT]AC^GT[GA] BsaWI 106 ACCGGA [AT]^CCGG[AT] BsiEI 1630 CGATCG CG[GA][CT]^CG BsrDI 3694 CATTGC GCAATG(2/0) BstEII 870 GGTGACC G^GT[ACGT]ACC BstPI 870 GGTGACC G^GT[ACGT]ACC BstXI 1364 CCAGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2307 CCTGAGG CC^T[ACGT]AGG DrdI 2873 GACTGTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2307 CCTGAGG CC^T[ACGT]AGG EcoO65I 870 GGTGACC G^GT[ACGT]ACC EcoT22I 3490 ATGCAT ATGCA^T FbaI 2405 TGATCA T^GATCA FspI 5040 TGCGCA TGC^GCA NaeI 1577 GCCGGC GCC^GGC NgoMIV 1577 GCCGGC G^CCGGC NsiI 3490 ATGCAT ATGCA^T PflFI 1503 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 216 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 299 ATTAAT AT^TAAT PvuI 1630 CGATCG CGAT^CG PvuII 3742 CAGCTG CAG^CTG SmaI 460 CCCGGG CCC^GGG StuI 3333 AGGCCT AGG^CCT Tth111I 1503 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1140 TCTAGA T^CTAGA XcmI 592 CCAATGAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 460 CCCGGG C^CCGGG XmaI 460 CCCGGG C^CCGGG XmnI 5014 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 536 CGCG CG^CG 1615 CGCG AhdI 1995 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4685 GACAGTGTGTC AspEI 1995 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4685 GACAGTGTGTC BsaBI 2661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2933 GATGATGATC BsgI 495 GTGCAG GTGCAG(16/14) 1837 GTGCAG BsmI 5014 GAATGC GAATGC(1/-1) 3755 GCATTC BspHI 272 TCATGA T^CATGA 4702 TCATGA BsrBI 126 GAGCGG CCGCTC(-3/-3) 597 GAGCGG BstUI 536 CGCG CG^CG 1615 CGCG DraIII 4982 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 5087 CACGTAGTG Eam1105I 1995 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4685 GACAGTGTGTC EcoRI 243 GAATTC G^AATTC 566 GAATTC EcoRV 987 GATATC GAT^ATC 4811 GATATC MamI 2661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2933 GATGATGATC MspA1I 999 CAGCGG C[AC]G^C[GT]G 3742 CAGCTG MvnI 536 CGCG CG^CG 1615 CGCG NspI 5037 GCATGC [GA]CATG^[CT] 5046 GCATGC PflMI 2900 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4003 CCACATCCTGG RcaI 272 TCATGA T^CATGA 4702 TCATGA SacI 1359 GAGCTC GAGCT^C 1394 GAGCTC SmlI 423 CTCAAG C^T[CT][GA]AG 3600 CTCAAG SphI 5037 GCATGC GCATG^C 5046 GCATGC SspI 1962 AATATT AAT^ATT 4805 AATATT TaqI 3498 TCGA T^CGA 4150 TCGA TthHB8I 3498 TCGA T^CGA 4150 TCGA Van91I 2900 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4003 CCACATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII