##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI AscI AseI
AsnI Asp718I AspEI BamHI BbeI
BbrPI BsaAI BsaWI BseAI BsiWI
BsmI BspEI BssHII Bst1107I BstUI
BstZ17I CpoI DrdI Eam1105I HindIII
KasI KpnI KspI MluI MroI
MvnI NarI NdeI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfoI
SgrAI SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 496 CTTAAG C^TTAAG
Aor51HI 785 AGCGCT AGC^GCT
AspI 2471 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 97 TGCGCA TGC^GCA
AvrII 3703 CCTAGG C^CTAGG
BbvCI 3944 GCTGAGG CCTCAGC(-5/-2)
BclI 912 TGATCA T^GATCA
BfrI 496 CTTAAG C^TTAAG
BlnI 3703 CCTAGG C^CTAGG
BlpI 1836 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1836 GCTGAGC GC^T[ACGT]AGC
BsaBI 4295 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 2257 ATCGAT AT^CGAT
BspLU11I 737 ACATGT A^CATGT
BssSI 766 CACGAG CACGAG(-5/-1)
BstBI 5129 TTCGAA TT^CGAA
BstEII 1115 GGTTACC G^GT[ACGT]ACC
BstPI 1115 GGTTACC G^GT[ACGT]ACC
CelII 1836 GCTGAGC GC^T[ACGT]AGC
ClaI 2257 ATCGAT AT^CGAT
DraI 3374 TTTAAA TTT^AAA
Eco47III 785 AGCGCT AGC^GCT
EcoO65I 1115 GGTTACC G^GT[ACGT]ACC
EcoRI 3261 GAATTC G^AATTC
FbaI 912 TGATCA T^GATCA
FspI 97 TGCGCA TGC^GCA
HpaI 2432 GTTAAC GTT^AAC
MamI 4295 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2427 CAATTG C^AATTG
MunI 2427 CAATTG C^AATTG
NheI 2507 GCTAGC G^CTAGC
NspV 5129 TTCGAA TT^CGAA
PflFI 2471 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4131 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 318 CCTGCAGG CCTGCA^GG
SfiI 270 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5129 TTCGAA TT^CGAA
Sse8387I 318 CCTGCAGG CCTGCA^GG
Tth111I 2471 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3674 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 3261 GAATTC [GA]^AATT[CT]
4335 AAATTT
AflIII 737 ACATGT A^C[GA][CT]GT
4880 ACGTGT
Alw44I 157 GTGCAC G^TGCAC
1463 GTGCAC
ApaI 1314 GGGCCC GGGCC^C
3028 GGGCCC
ApaLI 157 GTGCAC G^TGCAC
1463 GTGCAC
ApoI 3261 GAATTC [GA]^AATT[CT]
4335 AAATTT
BcgI 1352 CGACTCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3385 CGAGCGCACTGC
BciVI 642 GTATCC GTATCC(6/5)
950 GTATCC
BglI 271 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4019 GCCTCTTGGGC
BglII 800 AGATCT A^GATCT
4725 AGATCT
BspHI 746 TCATGA T^CATGA
2309 TCATGA
BsrBI 2122 GAGCGG CCGCTC(-3/-3)
4739 GAGCGG
DraIII 329 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1544 CACCCCGTG
EagI 1526 CGGCCG C^GGCCG
5062 CGGCCG
EclXI 1526 CGGCCG C^GGCCG
5062 CGGCCG
Eco52I 1526 CGGCCG C^GGCCG
5062 CGGCCG
EcoRV 550 GATATC GAT^ATC
2152 GATATC
EcoT22I 3323 ATGCAT ATGCA^T
4000 ATGCAT
FseI 541 GGCCGGCC GGCCGG^CC
1527 GGCCGGCC
HincII 2432 GTTAAC GT[CT]^[GA]AC
2830 GTTGAC
HindII 2432 GTTAAC GT[CT]^[GA]AC
2830 GTTGAC
MaeII 2956 ACGT A^CGT
4880 ACGT
MslI 2787 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3035 CATACCCGTG
NsiI 3323 ATGCAT ATGCA^T
4000 ATGCAT
RcaI 746 TCATGA T^CATGA
2309 TCATGA
SapI 1609 GAAGAGC GCTCTTC(1/4)
621 GCTCTTC
SspI 4321 AATATT AAT^ATT
5177 AATATT
StuI 2650 AGGCCT AGG^CCT
4173 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI