##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
ApaI AscI Asp718I AspEI AviII
BamHI BbeI BcgI BciVI BclI
BglI BsaWI BseAI BsiEI BsmBI
BspDI BspEI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
FbaI FseI FspI HpaI KasI
KpnI KspI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SphI Sse8387I SspI StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 835 GACGTC GACGT^C
AccI 2450 GTAGAC GT^[AC][GT]AC
AccII 4487 CGCG CG^CG
AcyI 835 GACGTC G[GA]^CG[CT]C
Aor51HI 1605 AGCGCT AGC^GCT
AseI 1949 ATTAAT AT^TAAT
AsnI 1949 ATTAAT AT^TAAT
Asp700I 4886 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5341 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1346 CCCGAG C^[CT]CG[GA]G
BalI 189 TGGCCA TGG^CCA
BanI 310 GGTGCC G^G[CT][GA]CC
BbrPI 1571 CACGTG CAC^GTG
BsaBI 4116 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 835 GACGTC G[GA]^CG[CT]C
BsiWI 2703 CGTACG C^GTACG
BsoBI 1346 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4791 TGTACA T^GTACA
BsrGI 4791 TGTACA T^GTACA
BssSI 889 CTCGTG CACGAG(-5/-1)
BstAPI 5165 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4487 CGCG CG^CG
Eco47III 1605 AGCGCT AGC^GCT
EcoRV 4766 GATATC GAT^ATC
EcoT22I 2129 ATGCAT ATGCA^T
HgaI 4485 GACGC GACGC(5/10)
Hin1I 835 GACGTC G[GA]^CG[CT]C
MamI 4116 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4486 ACGCGT A^CGCGT
MluNI 189 TGGCCA TGG^CCA
MscI 189 TGGCCA TGG^CCA
MvnI 4487 CGCG CG^CG
NdeI 2326 CATATG CA^TATG
NheI 3797 GCTAGC G^CTAGC
NsiI 2129 ATGCAT ATGCA^T
PflFI 5341 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4199 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1571 CACGTG CAC^GTG
PmlI 1571 CACGTG CAC^GTG
PshAI 4022 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1949 ATTAAT AT^TAAT
PvuII 1739 CAGCTG CAG^CTG
SapI 508 GCTCTTC GCTCTTC(1/4)
SnaBI 2154 TACGTA TAC^GTA
SpeI 2637 ACTAGT A^CTAGT
SspBI 4791 TGTACA T^GTACA
Tth111I 5341 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4199 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 4886 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 657 CCTGAGG CC^T[ACGT]AGG
2074 CCTTAGG
AvrII 2888 CCTAGG C^CTAGG
4955 CCTAGG
BcnI 1391 CCCGG CC^[GC]GG
5516 CCGGG
BglII 690 AGATCT A^GATCT
950 AGATCT
BlnI 2888 CCTAGG C^CTAGG
4955 CCTAGG
BpmI 3623 CTCCAG CTGGAG(16/14)
1226 CTGGAG
BsmFI 4997 GGGAC GGGAC(10/14)
5256 GGGAC
BsmI 2669 GAATGC GAATGC(1/-1)
3084 GAATGC
BspLU11I 3913 ACATGT A^CATGT
5568 ACATGT
BstXI 438 CCATGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCAACGCTCTGG
Bsu36I 657 CCTGAGG CC^T[ACGT]AGG
2074 CCTTAGG
EaeI 189 TGGCCA [CT]^GGCC[GA]
215 TGGCCG
Eco81I 657 CCTGAGG CC^T[ACGT]AGG
2074 CCTTAGG
EcoNI 1215 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2152 CCTACGTAAGG
FauI 2715 CCCGC CCCGC(4/6)
4543 GCGGG
HaeII 1105 GGCGCT [GA]GCGC^[CT]
1605 AGCGCT
HapII 1392 CCGG C^CGG
5516 CCGG
HincII 1015 GTCAAC GT[CT]^[GA]AC
4916 GTTGAC
HindII 1015 GTCAAC GT[CT]^[GA]AC
4916 GTTGAC
HindIII 5271 AAGCTT A^AGCTT
5491 AAGCTT
HpaII 1392 CCGG C^CGG
5516 CCGG
MfeI 4570 CAATTG C^AATTG
5596 CAATTG
MspI 1392 CCGG C^CGG
5516 CCGG
MunI 4570 CAATTG C^AATTG
5596 CAATTG
NciI 1391 CCCGG CC^[GC]GG
5516 CCGGG
NcoI 192 CCATGG C^CATGG
848 CCATGG
PpuMI 5256 GGGACCT [GA]G^G[AT]CC[CT]
5339 AGGACCC
TaqI 620 TCGA T^CGA
1021 TCGA
TthHB8I 620 TCGA T^CGA
1021 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaII BanII BbsI
BbvCI BbvI BfaI BfrI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII