################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AgeI AhdI AlwNI AocI ApaI AscI Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbrPI BcgI BcnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AclI 1218 AACGTT AA^CGTT AflII 558 CTTAAG C^TTAAG Alw44I 4470 GTGCAC G^TGCAC Aor51HI 659 AGCGCT AGC^GCT ApaLI 4470 GTGCAC G^TGCAC AseI 5579 ATTAAT AT^TAAT AsnI 5579 ATTAAT AT^TAAT Asp700I 3829 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 3985 CCTAGG C^CTAGG BalI 2614 TGGCCA TGG^CCA BciVI 3293 GTATCC GTATCC(6/5) BfrI 558 CTTAAG C^TTAAG BglI 3661 GCCACATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3985 CCTAGG C^CTAGG BsmI 3082 GAATGC GAATGC(1/-1) BspDI 3399 ATCGAT AT^CGAT BspHI 2235 TCATGA T^CATGA BstAPI 457 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 660 GCGC GCG^C ClaI 3399 ATCGAT AT^CGAT DraIII 1244 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4291 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 659 AGCGCT AGC^GCT EcoRI 3262 GAATTC G^AATTC EcoT22I 5288 ATGCAT ATGCA^T FauI 23 GCGGG CCCGC(4/6) HaeII 659 AGCGCT [GA]GCGC^[CT] HhaI 660 GCGC GCG^C HinP1I 660 GCGC G^CGC MluNI 2614 TGGCCA TGG^CCA MscI 2614 TGGCCA TGG^CCA NcoI 2159 CCATGG C^CATGG NsiI 5288 ATGCAT ATGCA^T PflMI 1334 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 5183 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5579 ATTAAT AT^TAAT Psp1406I 1218 AACGTT AA^CGTT RcaI 2235 TCATGA T^CATGA ScaI 3422 AGTACT AGT^ACT SpeI 137 ACTAGT A^CTAGT SphI 453 GCATGC GCATG^C TaqI 3400 TCGA T^CGA TthHB8I 3400 TCGA T^CGA Van91I 1334 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3829 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BanII 210 GAGCTC G[GA]GC[CT]^C 4061 GAGCTC BbvCI 798 CCTCAGC CCTCAGC(-5/-2) 569 GCTGAGG BclI 822 TGATCA T^GATCA 1263 TGATCA BsaBI 4793 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5673 GATTGGTATC BsgI 1424 CTGCAC GTGCAG(16/14) 2564 GTGCAG Bsp1407I 2075 TGTACA T^GTACA 5620 TGTACA BspLU11I 1141 ACATGT A^CATGT 5554 ACATGT BsrGI 2075 TGTACA T^GTACA 5620 TGTACA DsaI 1248 CCGTGG C^C[GA][CT]GG 2159 CCATGG EaeI 505 TGGCCG [CT]^GGCC[GA] 2614 TGGCCA FbaI 822 TGATCA T^GATCA 1263 TGATCA HincII 2466 GTTGAC GT[CT]^[GA]AC 5456 GTTGAC HindII 2466 GTTGAC GT[CT]^[GA]AC 5456 GTTGAC MamI 4793 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5673 GATTGGTATC NheI 3188 GCTAGC G^CTAGC 4099 GCTAGC PpuMI 46 AGGACCC [GA]G^G[AT]CC[CT] 4311 AGGACCT SacI 210 GAGCTC GAGCT^C 4061 GAGCTC SexAI 962 ACCTGGT A^CC[AT]GGT 3757 ACCTGGT SspBI 2075 TGTACA T^GTACA 5620 TGTACA SspI 1940 AATATT AAT^ATT 5125 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XcmI XhoII