##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI AlwNI AocI
ApaI AscI Asp718I AspEI AspI
AvaI AviII BamHI BanI BbeI
BbrPI BcgI BcnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I FseI FspI HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 1218 AACGTT AA^CGTT
AflII 558 CTTAAG C^TTAAG
Alw44I 4470 GTGCAC G^TGCAC
Aor51HI 659 AGCGCT AGC^GCT
ApaLI 4470 GTGCAC G^TGCAC
AseI 5579 ATTAAT AT^TAAT
AsnI 5579 ATTAAT AT^TAAT
Asp700I 3829 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3985 CCTAGG C^CTAGG
BalI 2614 TGGCCA TGG^CCA
BciVI 3293 GTATCC GTATCC(6/5)
BfrI 558 CTTAAG C^TTAAG
BglI 3661 GCCACATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3985 CCTAGG C^CTAGG
BsmI 3082 GAATGC GAATGC(1/-1)
BspDI 3399 ATCGAT AT^CGAT
BspHI 2235 TCATGA T^CATGA
BstAPI 457 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 660 GCGC GCG^C
ClaI 3399 ATCGAT AT^CGAT
DraIII 1244 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4291 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 659 AGCGCT AGC^GCT
EcoRI 3262 GAATTC G^AATTC
EcoT22I 5288 ATGCAT ATGCA^T
FauI 23 GCGGG CCCGC(4/6)
HaeII 659 AGCGCT [GA]GCGC^[CT]
HhaI 660 GCGC GCG^C
HinP1I 660 GCGC G^CGC
MluNI 2614 TGGCCA TGG^CCA
MscI 2614 TGGCCA TGG^CCA
NcoI 2159 CCATGG C^CATGG
NsiI 5288 ATGCAT ATGCA^T
PflMI 1334 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5183 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5579 ATTAAT AT^TAAT
Psp1406I 1218 AACGTT AA^CGTT
RcaI 2235 TCATGA T^CATGA
ScaI 3422 AGTACT AGT^ACT
SpeI 137 ACTAGT A^CTAGT
SphI 453 GCATGC GCATG^C
TaqI 3400 TCGA T^CGA
TthHB8I 3400 TCGA T^CGA
Van91I 1334 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3829 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 210 GAGCTC G[GA]GC[CT]^C
4061 GAGCTC
BbvCI 798 CCTCAGC CCTCAGC(-5/-2)
569 GCTGAGG
BclI 822 TGATCA T^GATCA
1263 TGATCA
BsaBI 4793 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5673 GATTGGTATC
BsgI 1424 CTGCAC GTGCAG(16/14)
2564 GTGCAG
Bsp1407I 2075 TGTACA T^GTACA
5620 TGTACA
BspLU11I 1141 ACATGT A^CATGT
5554 ACATGT
BsrGI 2075 TGTACA T^GTACA
5620 TGTACA
DsaI 1248 CCGTGG C^C[GA][CT]GG
2159 CCATGG
EaeI 505 TGGCCG [CT]^GGCC[GA]
2614 TGGCCA
FbaI 822 TGATCA T^GATCA
1263 TGATCA
HincII 2466 GTTGAC GT[CT]^[GA]AC
5456 GTTGAC
HindII 2466 GTTGAC GT[CT]^[GA]AC
5456 GTTGAC
MamI 4793 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5673 GATTGGTATC
NheI 3188 GCTAGC G^CTAGC
4099 GCTAGC
PpuMI 46 AGGACCC [GA]G^G[AT]CC[CT]
4311 AGGACCT
SacI 210 GAGCTC GAGCT^C
4061 GAGCTC
SexAI 962 ACCTGGT A^CC[AT]GGT
3757 ACCTGGT
SspBI 2075 TGTACA T^GTACA
5620 TGTACA
SspI 1940 AATATT AAT^ATT
5125 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XcmI
XhoII